ID   CRG1_YEAST              Reviewed;         291 AA.
AC   P38892; D3DLF8; Q6Q5R3;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   04-NOV-2026, entry version 153.
DE   RecName: Full=Probable S-adenosylmethionine-dependent methyltransferase CRG1;
DE            EC=2.1.1.-;
DE   AltName: Full=Cantharidin resistance protein 1;
GN   Name=CRG1; OrderedLocusNames=YHR209W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8091229; DOI=10.1126/science.8091229;
RA   Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA   Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA   Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA   Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA   St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA   Waterston R., Wilson R., Vaudin M.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT   VIII.";
RL   Science 265:2077-2082(1994).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   FUNCTION.
RX   PubMed=9873020; DOI=10.1074/jbc.274.2.814;
RA   Niewmierzycka A., Clarke S.;
RT   "S-adenosylmethionine-dependent methylation in Saccharomyces cerevisiae.
RT   Identification of a novel protein arginine methyltransferase.";
RL   J. Biol. Chem. 274:814-824(1999).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   INDUCTION.
RX   PubMed=15116342; DOI=10.1002/yea.1109;
RA   Boorsma A., de Nobel H., ter Riet B., Bargmann B., Brul S.,
RA   Hellingwerf K.J., Klis F.M.;
RT   "Characterization of the transcriptional response to cell wall stress in
RT   Saccharomyces cerevisiae.";
RL   Yeast 21:413-427(2004).
RN   [8]
RP   FUNCTION.
RX   PubMed=18622389; DOI=10.1038/nchembio.100;
RA   Hoon S., Smith A.M., Wallace I.M., Suresh S., Miranda M., Fung E.,
RA   Proctor M., Shokat K.M., Zhang C., Davis R.W., Giaever G., St Onge R.P.,
RA   Nislow C.;
RT   "An integrated platform of genomic assays reveals small-molecule
RT   bioactivities.";
RL   Nat. Chem. Biol. 4:498-506(2008).
CC   -!- FUNCTION: Probable S-adenosylmethionine-dependent methyltransferase
CC       which mediates cantharidin resistance. {ECO:0000269|PubMed:18622389,
CC       ECO:0000269|PubMed:9873020}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC   -!- INDUCTION: By cell wall perturbation. {ECO:0000269|PubMed:15116342}.
CC   -!- MISCELLANEOUS: Present with 4380 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; U00029; AAB69732.1; -; Genomic_DNA.
DR   EMBL; AY557767; AAS56093.1; -; Genomic_DNA.
DR   EMBL; BK006934; DAA06902.1; -; Genomic_DNA.
DR   PIR; S48990; S48990.
DR   RefSeq; NP_012079.1; NM_001179340.1.
DR   AlphaFoldDB; P38892; -.
DR   SMR; P38892; -.
DR   BioGRID; 36643; 97.
DR   DIP; DIP-828N; -.
DR   FunCoup; P38892; 967.
DR   IntAct; P38892; 1.
DR   STRING; 4932.YHR209W; -.
DR   iPTMnet; P38892; -.
DR   PaxDb; 4932-YHR209W; -.
DR   PeptideAtlas; P38892; -.
DR   EnsemblFungi; YHR209W_mRNA; YHR209W; YHR209W.
DR   GeneID; 856616; -.
DR   KEGG; sce:YHR209W; -.
DR   AGR; SGD:S000001252; -.
DR   SGD; S000001252; CRG1.
DR   VEuPathDB; FungiDB:YHR209W; -.
DR   eggNOG; KOG3010; Eukaryota.
DR   HOGENOM; CLU_049344_1_2_1; -.
DR   InParanoid; P38892; -.
DR   OMA; AAGPYWE; -.
DR   OrthoDB; 10027013at2759; -.
DR   BioCyc; YEAST:G3O-31234-MONOMER; -.
DR   BioGRID-ORCS; 856616; 1 hit in 10 CRISPR screens.
DR   PRO; PR:P38892; -.
DR   Proteomes; UP000002311; Chromosome VIII.
DR   RNAct; P38892; protein.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IMP:SGD.
DR   GO; GO:0003729; F:mRNA binding; IDA:SGD.
DR   GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IDA:SGD.
DR   GO; GO:0055088; P:lipid homeostasis; IMP:SGD.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   FunFam; 3.40.50.150:FF:000482; Probable S-adenosylmethionine-dependent methyltransferase CRG1; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR051052; Diverse_substrate_MTase.
DR   InterPro; IPR013216; Methyltransf_11.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR44942; METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR44942:SF4; METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF08241; Methyltransf_11; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..291
FT                   /note="Probable S-adenosylmethionine-dependent
FT                   methyltransferase CRG1"
FT                   /id="PRO_0000202943"
FT   CONFLICT        23
FT                   /note="Y -> C (in Ref. 3; AAS56093)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   291 AA;  33785 MW;  C1DFE5F73BAAC617 CRC64;
     MPKTSYLNKN FESAHYNNVR PSYPLSLVNE IMKFHKGTRK SLVDIGCGTG KATFVVEPYF
     KEVIGIDPSS AMLSIAEKET NERRLDKKIR FINAPGEDLS SIRPESVDMV ISAEAIHWCN
     LERLFQQVSS ILRSDGTFAF WFYIQPEFVD FPEALNVYYK YGWSKDYMGK YLNDNQREIL
     LNYGGEKLRS LLSDRFGDIE VTIYSPSDPN ASTVTAENSQ FLWRAAITLN QFKEFVKSWS
     IYTSWARDNP SKPDIADIFI NELKEICHCE DLNVPLKIEW STFYYLCRKR E
//