ID   MIX23_YEAST             Reviewed;         196 AA.
AC   P38162; D6VPP6;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   04-NOV-2026, entry version 141.
DE   RecName: Full=Mitochondrial intermembrane space cysteine motif-containing protein MIX23 {ECO:0000303|PubMed:32826315};
DE   AltName: Full=Mitochondrial intermembrane space CX(n)C motif protein of 23 kDa {ECO:0000303|PubMed:32826315};
GN   Name=MIX23 {ECO:0000312|SGD:S000000203};
GN   Synonyms=MIC23 {ECO:0000303|PubMed:22984289};
GN   OrderedLocusNames=YBL107C {ECO:0000303|PubMed:22984289};
GN   ORFNames=YBL0805;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7502586; DOI=10.1002/yea.320111112;
RA   Obermaier B., Gassenhuber J., Piravandi E., Domdey H.;
RT   "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces
RT   cerevisiae chromosome II.";
RL   Yeast 11:1103-1112(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA   Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA   Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA   Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA   Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA   Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA   Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA   Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA   Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA   Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA   Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA   Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA   Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA   Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA   Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA   Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA   Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA   Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA   Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA   Mewes H.-W., Kleine K.;
RT   "Complete DNA sequence of yeast chromosome II.";
RL   EMBO J. 13:5795-5809(1994).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION [LARGE SCALE
RP   ANALYSIS], AND DOMAIN.
RX   PubMed=22984289; DOI=10.1074/mcp.m112.021105;
RA   Voegtle F.N., Burkhart J.M., Rao S., Gerbeth C., Hinrichs J.,
RA   Martinou J.C., Chacinska A., Sickmann A., Zahedi R.P., Meisinger C.;
RT   "Intermembrane space proteome of yeast mitochondria.";
RL   Mol. Cell. Proteomics 11:1840-1852(2012).
RN   [6]
RP   GENE NAME.
RX   PubMed=24687277; DOI=10.1083/jcb.201401006;
RA   Pfanner N., van der Laan M., Amati P., Capaldi R.A., Caudy A.A.,
RA   Chacinska A., Darshi M., Deckers M., Hoppins S., Icho T., Jakobs S., Ji J.,
RA   Kozjak-Pavlovic V., Meisinger C., Odgren P.R., Park S.K., Rehling P.,
RA   Reichert A.S., Sheikh M.S., Taylor S.S., Tsuchida N., van der Bliek A.M.,
RA   van der Klei I.J., Weissman J.S., Westermann B., Zha J., Neupert W.,
RA   Nunnari J.;
RT   "Uniform nomenclature for the mitochondrial contact site and cristae
RT   organizing system.";
RL   J. Cell Biol. 204:1083-1086(2014).
RN   [7]
RP   FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY RPN4, AND MUTAGENESIS OF
RP   CYS-38; CYS-77; CYS-99; CYS-114; CYS-178 AND CYS-192.
RX   PubMed=32826315; DOI=10.1074/jbc.ra120.014247;
RA   Zoeller E., Laborenz J., Kraemer L., Boos F., Raeschle M., Alexander R.T.,
RA   Herrmann J.M.;
RT   "The intermembrane space protein Mix23 is a novel stress-induced
RT   mitochondrial import factor.";
RL   J. Biol. Chem. 295:14686-14697(2020).
CC   -!- FUNCTION: Regulator of the mitochondrial protein import machinery that
CC       is localized in the mitochondrial intermembrane space (IMS) and
CC       facilitates the transport of proteins from the cytosol into the
CC       mitochondrial matrix (PubMed:32826315). Not essential for mitochondrial
CC       protein import but induced and required when mitochondrial import is
CC       compromised (PubMed:32826315). Stimulates or stabilizes the
CC       translocation into the mitochondria of proteins such as OXA1, ATP1 and
CC       COX12 (PubMed:32826315). {ECO:0000269|PubMed:32826315}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion intermembrane space
CC       {ECO:0000269|PubMed:22984289, ECO:0000269|PubMed:32826315}.
CC       Note=Imported into the mitochondria via the mitochondrial MIA40-ERV1
CC       machinery. {ECO:0000269|PubMed:22984289, ECO:0000269|PubMed:32826315}.
CC   -!- INDUCTION: Up-regulated by RPN4 transcription factor upon mitochondrial
CC       protein-induced stress conditions such as the accumulation in the
CC       cytosol of non-imported mitochondrial precursors.
CC       {ECO:0000269|PubMed:32826315}.
CC   -!- DOMAIN: The Cx14C/Cx13C motifs are involved in the recognition by the
CC       mitochondrial MIA40-ERV1 disulfide relay system and the subsequent
CC       transfer of disulfide bonds by dithiol/disulfide exchange reactions to
CC       the newly imported protein. {ECO:0000269|PubMed:22984289}.
CC   -!- MISCELLANEOUS: Present with 768 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the MIX23 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X79489; CAA55988.1; -; Genomic_DNA.
DR   EMBL; Z35868; CAA84934.1; -; Genomic_DNA.
DR   EMBL; BK006936; DAA07016.1; -; Genomic_DNA.
DR   PIR; S45388; S45388.
DR   RefSeq; NP_009443.1; NM_001178347.1.
DR   SMR; P38162; -.
DR   BioGRID; 32596; 49.
DR   DIP; DIP-3952N; -.
DR   IntAct; P38162; 1.
DR   STRING; 4932.YBL107C; -.
DR   PaxDb; 4932-YBL107C; -.
DR   PeptideAtlas; P38162; -.
DR   EnsemblFungi; YBL107C_mRNA; YBL107C; YBL107C.
DR   GeneID; 852167; -.
DR   KEGG; sce:YBL107C; -.
DR   VEuPathDB; FungiDB:YBL107C; -.
DR   AGR; SGD:S000000203; -.
DR   SGD; S000000203; MIX23.
DR   eggNOG; ENOG502S17Q; Eukaryota.
DR   HOGENOM; CLU_118733_0_0_1; -.
DR   InParanoid; P38162; -.
DR   OMA; AACNAFK; -.
DR   OrthoDB; 5593818at2759; -.
DR   BioCyc; YEAST:G3O-28991-MONOMER; -.
DR   PRO; PR:P38162; -.
DR   InterPro; IPR019171; MIX23.
DR   InterPro; IPR016805; MIX23_fungal.
DR   PANTHER; PTHR31905; COILED-COIL DOMAIN-CONTAINING PROTEIN 58; 1.
DR   PANTHER; PTHR31905:SF2; PROTEIN MIX23; 1.
DR   Pfam; PF09774; MIX23; 1.
DR   PIRSF; PIRSF022603; UCP022603; 1.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005758; C:mitochondrial intermembrane space; IDA:FlyBase.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:1903749; P:positive regulation of protein localization to mitochondrion; IMP:UniProtKB.
DR   Proteomes; UP000002311; Chromosome II.
DR   RNAct; P38162; protein.
DR   BioGRID-ORCS; 852167; 1 hit in 10 CRISPR screens.
DR   AlphaFoldDB; P38162; -.
DR   FunCoup; P38162; 45.
PE   1: Evidence at protein level;
KW   Mitochondrion; Protein transport; Reference proteome; Transport.
FT   CHAIN           1..196
FT                   /note="Mitochondrial intermembrane space cysteine motif-
FT                   containing protein MIX23"
FT                   /id="PRO_0000202439"
FT   MOTIF           99..114
FT                   /note="Cx14C motif"
FT                   /evidence="ECO:0000303|PubMed:22984289"
FT   MOTIF           178..192
FT                   /note="Cx13C motif"
FT                   /evidence="ECO:0000303|PubMed:22984289"
FT   MUTAGEN         38
FT                   /note="C->S: Loss of MIA40-dependent import into the
FT                   mitochondrial intermembrane space; when associated with
FT                   S-77, S-99, S-114, S-178 and S-192."
FT                   /evidence="ECO:0000269|PubMed:32826315"
FT   MUTAGEN         77
FT                   /note="C->S: Loss of MIA40-dependent import into the
FT                   mitochondrial intermembrane space; when associated with
FT                   S-38, S-99, S-114, S-178 and S-192."
FT                   /evidence="ECO:0000269|PubMed:32826315"
FT   MUTAGEN         99
FT                   /note="C->S: Loss of MIA40-dependent import into the
FT                   mitochondrial intermembrane space; when associated with
FT                   S-38, S-77, S-114, S-178 and S-192."
FT                   /evidence="ECO:0000269|PubMed:32826315"
FT   MUTAGEN         114
FT                   /note="C->S: Loss of MIA40-dependent import into the
FT                   mitochondrial intermembrane space; when associated with
FT                   S-38, S-77, S-99, S-178 and S-192."
FT                   /evidence="ECO:0000269|PubMed:32826315"
FT   MUTAGEN         178
FT                   /note="C->S: Loss of MIA40-dependent import into the
FT                   mitochondrial intermembrane space; when associated with
FT                   S-38, S-77, S-99, S-114 and S-192."
FT                   /evidence="ECO:0000269|PubMed:32826315"
FT   MUTAGEN         192
FT                   /note="C->S: Loss of MIA40-dependent import into the
FT                   mitochondrial intermembrane space; when associated with
FT                   S-38, S-77, S-99, S-114 and S-178."
FT                   /evidence="ECO:0000269|PubMed:32826315"
SQ   SEQUENCE   196 AA;  22966 MW;  E54356AE336896B5 CRC64;
     MVDNRRTFTA PQSLLETNLT FPNDEPSLTT ITVTRERCVD PSLIDSFLRF LRHGSDDIIR
     QKLNNYRKGS INGKNKCKEF LKQELYPNWQ IRNNIISFCE KEAAEMKNET DQQCGNNKKT
     TAEPLIDARI DPYAARERAE KQEAQYKDWT KVTEWVANNR KIEQILTSTT EGILRQNCEQ
     NNDYLKEFTQ FCKDNS
//