ID   WECG_SALTY              Reviewed;         246 AA.
AC   P37457;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   04-NOV-2026, entry version 126.
DE   RecName: Full=UDP-N-acetyl-D-mannosaminuronic acid transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE            Short=UDP-ManNAcA transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE            EC=2.4.1.180 {ECO:0000255|HAMAP-Rule:MF_01001};
GN   Name=wecG {ECO:0000255|HAMAP-Rule:MF_01001};
GN   Synonyms=rffM {ECO:0000255|HAMAP-Rule:MF_01001};
GN   OrderedLocusNames=STM3929; ORFNames=STMD1.61;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8509342; DOI=10.1128/jb.175.12.3897-3899.1993;
RA   Lu C.D., Abdelal A.T.;
RT   "The Salmonella typhimurium uracil-sensitive mutation use is in argU and
RT   encodes a minor arginine tRNA.";
RL   J. Bacteriol. 175:3897-3899(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
CC   -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II),
CC       the second lipid-linked intermediate involved in enterobacterial common
CC       antigen (ECA) synthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-N-acetyl-alpha-D-mannosaminouronate + N-acetyl-alpha-D-
CC         glucosaminyl-di-trans,octa-cis-undecaprenyl diphosphate = beta-D-
CC         ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-undecaprenyl
CC         diphosphate + UDP + H(+); Xref=Rhea:RHEA:28366, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:61495, ChEBI:CHEBI:62959,
CC         ChEBI:CHEBI:70731; EC=2.4.1.180; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01001};
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC       antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 26 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01001}.
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DR   EMBL; M95047; AAA92025.1; -; Genomic_DNA.
DR   EMBL; AF233324; AAF33458.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL22778.1; -; Genomic_DNA.
DR   PIR; S27727; S27727.
DR   RefSeq; NP_462819.1; NC_003197.2.
DR   RefSeq; WP_000183621.1; NC_003197.2.
DR   AlphaFoldDB; P37457; -.
DR   SMR; P37457; -.
DR   STRING; 99287.STM3929; -.
DR   PaxDb; 99287-STM3929; -.
DR   DNASU; 1255455; -.
DR   GeneID; 1255455; -.
DR   KEGG; stm:STM3929; -.
DR   PATRIC; fig|99287.12.peg.4150; -.
DR   HOGENOM; CLU_063203_3_2_6; -.
DR   OMA; AAMDRWP; -.
DR   PhylomeDB; P37457; -.
DR   BioCyc; SENT99287:STM3929-MONOMER; -.
DR   UniPathway; UPA00566; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0016758; F:hexosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0047241; F:lipopolysaccharide N-acetylmannosaminouronosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd06533; Glyco_transf_WecG_TagA; 1.
DR   HAMAP; MF_01001; WecG_RffM; 1.
DR   InterPro; IPR023085; UDP-ManNAcA_Trfase_WecG.
DR   InterPro; IPR004629; WecG_TagA_CpsF.
DR   NCBIfam; NF002980; wecG; 1.
DR   NCBIfam; TIGR00696; wecG_tagA_cpsF; 1.
DR   PANTHER; PTHR34136; -; 1.
DR   PANTHER; PTHR34136:SF1; UDP-N-ACETYL-D-MANNOSAMINURONIC ACID TRANSFERASE; 1.
DR   Pfam; PF03808; Glyco_tran_WecG; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..246
FT                   /note="UDP-N-acetyl-D-mannosaminuronic acid transferase"
FT                   /id="PRO_0000208434"
SQ   SEQUENCE   246 AA;  27599 MW;  847009586988CF0D CRC64;
     MTNNAAAPLY SLRGLPLIGW RDMSHALNYL FADGQLKQGT LVAINAEKLL TAEDNPEVRA
     LIAAAEFKYA DGISVVRSIR KKFPQAQVSR VAGADLWEAL MARAGKEGTP VFLVGGKPEV
     LAQTEAKLRT QWNVNIVGSQ DGYFTPEQRQ ALFARIHASG AKIVTVAMGS PKQELLMRDC
     REVHPHALYM GVGGTYDVFT GHVKRAPKIW QNLGLEWLYR LLSQPRRITR QMRLLRYLRW
     HYTGDL
//