ID   PKG3_ADE12              Reviewed;         373 AA.
AC   P36715;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   02-SEP-2026, entry version 72.
DE   RecName: Full=Packaging protein 3 {ECO:0000255|HAMAP-Rule:MF_04058};
DE   AltName: Full=L1-52/55 kDa protein {ECO:0000255|HAMAP-Rule:MF_04058};
DE   AltName: Full=Packaging protein 52K {ECO:0000255|HAMAP-Rule:MF_04058};
GN   Name=L1 {ECO:0000255|HAMAP-Rule:MF_04058};
OS   Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12).
OC   Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Polisuviricotina;
OC   Pharingeaviricetes; Rowavirales; Adenoviridae; Mastadenovirus;
OC   Mastadenovirus adami; Human mastadenovirus A.
OX   NCBI_TaxID=28282;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8254750; DOI=10.1128/jvi.68.1.379-389.1994;
RA   Sprengel J., Schmitz B., Heuss-Neitzel D., Zock C., Doerfler W.;
RT   "Nucleotide sequence of human adenovirus type 12 DNA: comparative
RT   functional analysis.";
RL   J. Virol. 68:379-389(1994).
CC   -!- FUNCTION: Involved in viral genome packaging through its interaction
CC       with packaging proteins 1 and 2. After proteolytic cleavage by
CC       adenovirus protease, L1 52/55k protein is removed from the capsid
CC       during viral maturation. {ECO:0000255|HAMAP-Rule:MF_04058}.
CC   -!- SUBUNIT: Part of the genome packaging complex composed of packaging
CC       proteins 1, 2 and 3; this complex specifically binds to the packaging
CC       sequence on the left end of viral genomic DNA and performs packaging of
CC       the viral genome. Interacts with hexon-linking protein IIIa; this
CC       interaction is required to promote correct genome packaging.
CC       {ECO:0000255|HAMAP-Rule:MF_04058}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04058}.
CC       Note=Nuclear protein present in empty capsids and assembly
CC       intermediates. {ECO:0000255|HAMAP-Rule:MF_04058}.
CC   -!- INDUCTION: Expressed in the early phase and late phase of the viral
CC       replicative cycle. {ECO:0000255|HAMAP-Rule:MF_04058}.
CC   -!- PTM: Cleaved at different sites by the viral protease during virion
CC       maturation. {ECO:0000255|HAMAP-Rule:MF_04058}.
CC   -!- MISCELLANEOUS: All late proteins expressed from the major late promoter
CC       are produced by alternative splicing and alternative polyadenylation of
CC       the same gene giving rise to non-overlapping ORFs. A leader sequence is
CC       present in the N-terminus of all these mRNAs and is recognized by the
CC       viral shutoff protein to provide expression although conventional
CC       translation via ribosome scanning from the cap has been shut off in the
CC       host cell. {ECO:0000255|HAMAP-Rule:MF_04058}.
CC   -!- SIMILARITY: Belongs to the adenoviridae packaging protein 3 family.
CC       {ECO:0000255|HAMAP-Rule:MF_04058}.
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DR   EMBL; X73487; CAA51885.1; -; Genomic_DNA.
DR   PIR; S33936; S33936.
DR   RefSeq; NP_040918.1; NC_001460.1.
DR   AlphaFoldDB; P36715; -.
DR   SMR; P36715; -.
DR   GeneID; 1460848; -.
DR   Proteomes; UP000004993; Segment.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019073; P:viral DNA genome packaging; IEA:UniProtKB-UniRule.
DR   GO; GO:0019076; P:viral release from host cell; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04058; ADV_PKG3; 1.
DR   InterPro; IPR037536; ADV_PKG3.
DR   InterPro; IPR004292; L1-like.
DR   Pfam; PF03052; Adeno_52K; 1.
PE   3: Inferred from homology;
KW   Host nucleus; Late protein; Phosphoprotein; Reference proteome;
KW   Viral genome packaging; Viral release from host cell.
FT   CHAIN           1..373
FT                   /note="Packaging protein 3"
FT                   /id="PRO_0000221868"
FT   REGION          1..150
FT                   /note="Interaction with packaging protein 1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04058"
FT   REGION          1..32
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         52
FT                   /note="Phosphoserine; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04058"
FT   MOD_RES         334
FT                   /note="Phosphoserine; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04058"
SQ   SEQUENCE   373 AA;  42330 MW;  CAAEA61313A3A6E2 CRC64;
     MHPVLRQMRP QPRATTASAA VALSGSGEQE EPQCPTLELE EGEGIARLGA HSPERHPRVQ
     LARDSRVAFV PRQNMFRDNS GEEAEEMRDC RFRAGRELRR GFNRERLLRE EDFEPDEHSG
     ISSARAHVSA ANLVTAYEQT VTEERNFQKS FNNHVRTLIA REEVAIGLMH LWDFVEAYVH
     NPASKPLTAQ LFLIVQHSRD NETFRDAMLN IAEPQGRWLL DLINILQSIV VQERSLSLAD
     KVAAINYSML SLGKFYARKI YKSPYVPIDK EVKIDSFYMR MALKVLTLSD DLGVYRNDRI
     HKAVSASRRR ELSDKELMHS LQRALTGAGT EDESFFDMGA DLRWQPSARA LEAAGVASAD
     VTGDDDDEDQ YED
//