ID   SAA4_HUMAN              Reviewed;         130 AA.
AC   P35542; Q6FHJ4;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 2.
DT   02-SEP-2026, entry version 179.
DE   RecName: Full=Serum amyloid A-4 protein {ECO:0000305};
DE   AltName: Full=Constitutively expressed serum amyloid A protein;
DE            Short=C-SAA;
DE   Flags: Precursor;
GN   Name=SAA4 {ECO:0000303|PubMed:7686132, ECO:0000312|HGNC:HGNC:10516};
GN   Synonyms=CSAA;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT TYR-89.
RC   TISSUE=Liver;
RX   PubMed=1740433; DOI=10.1016/s0021-9258(19)50605-6;
RA   Whitehead A.S., Debeer M.C., Steel D.M., Rits M., Lelias J.M., Lane W.S.,
RA   Debeer F.C.;
RT   "Identification of novel members of the serum amyloid A protein superfamily
RT   as constitutive apolipoproteins of high density lipoprotein.";
RL   J. Biol. Chem. 267:3862-3867(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT TYR-89.
RX   PubMed=7686132; DOI=10.1006/geno.1993.1209;
RA   Steel D.M., Sellar G.C., Uhlar C.M., Simon S., Debeer F.C., Whitehead A.S.;
RT   "A constitutively expressed serum amyloid A protein gene (SAA4) is closely
RT   linked to, and shares structural similarities with, an acute-phase serum
RT   amyloid A protein gene (SAA2).";
RL   Genomics 16:447-454(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT TYR-89.
RX   PubMed=1439582; DOI=10.1111/j.1365-3083.1992.tb03131.x;
RA   Watson G., Coade S., Woo P.;
RT   "Analysis of the genomic and derived protein structure of a novel human
RT   serum amyloid A gene, SAA4.";
RL   Scand. J. Immunol. 36:703-712(1992).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT TYR-89.
RA   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT   "Cloning of human full open reading frames in Gateway(TM) system entry
RT   vector (pDONR201).";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16554811; DOI=10.1038/nature04632;
RA   Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA   Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA   Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA   Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA   Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA   Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT   "Human chromosome 11 DNA sequence and analysis including novel gene
RT   identification.";
RL   Nature 440:497-500(2006).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT TYR-89.
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT TYR-89.
RC   TISSUE=Liver;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [8]
RP   PARTIAL PROTEIN SEQUENCE.
RX   PubMed=8783012; DOI=10.1002/elps.1150170508;
RA   Ducret A., Bruun C.F., Bures E.J., Marhaug G., Husby G., Aebersold R.;
RT   "Characterization of human serum amyloid A protein isoforms separated by
RT   two-dimensional electrophoresis by liquid chromatography/electrospray
RT   ionization tandem mass spectrometry.";
RL   Electrophoresis 17:866-876(1996).
RN   [9]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-94.
RC   TISSUE=Plasma;
RX   PubMed=16335952; DOI=10.1021/pr0502065;
RA   Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J.,
RA   Smith R.D.;
RT   "Human plasma N-glycoproteome analysis by immunoaffinity subtraction,
RT   hydrazide chemistry, and mass spectrometry.";
RL   J. Proteome Res. 4:2070-2080(2005).
RN   [10]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-94.
RC   TISSUE=Liver;
RX   PubMed=19159218; DOI=10.1021/pr8008012;
RA   Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT   "Glycoproteomics analysis of human liver tissue by combination of multiple
RT   enzyme digestion and hydrazide chemistry.";
RL   J. Proteome Res. 8:651-661(2009).
RN   [11]
RP   VARIANT TYR-89, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=22028381; DOI=10.1093/jmcb/mjr024;
RA   Su Z.D., Sun L., Yu D.X., Li R.X., Li H.X., Yu Z.J., Sheng Q.H., Lin X.,
RA   Zeng R., Wu J.R.;
RT   "Quantitative detection of single amino acid polymorphisms by targeted
RT   proteomics.";
RL   J. Mol. Cell Biol. 3:309-315(2011).
CC   -!- FUNCTION: Major acute phase reactant. {ECO:0000250|UniProtKB:P05366}.
CC   -!- SUBUNIT: Apolipoprotein of the HDL complex.
CC       {ECO:0000250|UniProtKB:P0DJI8}.
CC   -!- INTERACTION:
CC       P35542; Q9UHD4: CIDEB; NbExp=3; IntAct=EBI-750242, EBI-7062247;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P0DJI8}.
CC   -!- TISSUE SPECIFICITY: Expressed by the liver; secreted in plasma.
CC   -!- INDUCTION: Constitutively expressed.
CC   -!- SIMILARITY: Belongs to the SAA family. {ECO:0000305}.
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DR   EMBL; L05920; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; M81349; AAA60298.1; -; mRNA.
DR   EMBL; S48983; AAB24060.1; -; Genomic_DNA.
DR   EMBL; S48980; AAB24060.1; JOINED; Genomic_DNA.
DR   EMBL; S48981; AAB24060.1; JOINED; Genomic_DNA.
DR   EMBL; CR541758; CAG46558.1; -; mRNA.
DR   EMBL; AC090099; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471064; EAW68415.1; -; Genomic_DNA.
DR   EMBL; BC007026; AAH07026.1; -; mRNA.
DR   CCDS; CCDS7832.1; -.
DR   PIR; A38974; A38974.
DR   RefSeq; NP_006503.2; NM_006512.4.
DR   AlphaFoldDB; P35542; -.
DR   SMR; P35542; -.
DR   BioGRID; 112199; 11.
DR   FunCoup; P35542; 45.
DR   IntAct; P35542; 4.
DR   NDEx; IQUERY-CP-SAA4; 3 NDEx IQuery Curated Pathways.
DR   STRING; 9606.ENSP00000278222; -.
DR   GlyConnect; P35542; 2 N-Linked glycans (1 site).
DR   GlyCosmos; P35542; 1 site, 3 glycans.
DR   GlyGen; P35542; 1 site, 3 N-linked glycans (1 site).
DR   iPTMnet; P35542; -.
DR   PhosphoSitePlus; P35542; -.
DR   BioMuta; SAA4; -.
DR   DMDM; 296452894; -.
DR   jPOST; P35542; -.
DR   MassIVE; P35542; -.
DR   PaxDb; 9606-ENSP00000278222; -.
DR   PeptideAtlas; P35542; -.
DR   ProteomicsDB; 55078; -.
DR   TopDownProteomics; P35542; -.
DR   Antibodypedia; 12214; 297 antibodies from 38 providers.
DR   DNASU; 6291; -.
DR   Ensembl; ENST00000278222.7; ENSP00000278222.4; ENSG00000148965.11.
DR   Ensembl; ENST00000889031.1; ENSP00000559090.1; ENSG00000148965.11.
DR   Ensembl; ENST00000889034.1; ENSP00000559093.1; ENSG00000148965.11.
DR   Ensembl; ENST00000889035.1; ENSP00000559094.1; ENSG00000148965.11.
DR   Ensembl; ENST00000889036.1; ENSP00000559095.1; ENSG00000148965.11.
DR   GeneID; 6291; -.
DR   KEGG; hsa:6291; -.
DR   MANE-Select; ENST00000278222.7; ENSP00000278222.4; NM_006512.4; NP_006503.2.
DR   UCSC; uc001mny.4; human.
DR   AGR; HGNC:10516; -.
DR   ClinPGx; PA34924; -.
DR   CTD; 6291; -.
DR   DisGeNET; 6291; -.
DR   GeneCards; SAA4; -.
DR   HGNC; HGNC:10516; SAA4.
DR   HPA; ENSG00000148965; Tissue enriched (liver).
DR   MIM; 104752; gene.
DR   OpenTargets; ENSG00000148965; -.
DR   VEuPathDB; HostDB:ENSG00000148965; -.
DR   eggNOG; ENOG502S4PB; Eukaryota.
DR   GeneTree; ENSGT00390000004737; -.
DR   InParanoid; P35542; -.
DR   OrthoDB; 6112826at2759; -.
DR   PAN-GO; P35542; 0 GO annotations based on evolutionary models.
DR   PhylomeDB; P35542; -.
DR   PathwayCommons; P35542; -.
DR   SignaLink; P35542; -.
DR   Agora; ENSG00000148965; -.
DR   BioGRID-ORCS; 6291; 9 hits in 1138 CRISPR screens.
DR   GenomeRNAi; 6291; -.
DR   Pharos; P35542; Tbio.
DR   PRO; PR:P35542; -.
DR   Proteomes; UP000005640; Chromosome 11.
DR   RNAct; P35542; protein.
DR   Bgee; ENSG00000148965; Expressed in right lobe of liver and 77 other cell types or tissues.
DR   GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR   GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR   FunFam; 1.10.132.110:FF:000001; Serum amyloid A protein; 1.
DR   Gene3D; 1.10.132.110; Serum amyloid A protein; 1.
DR   InterPro; IPR000096; Serum_amyloid_A.
DR   InterPro; IPR052464; Synovial_Prolif_Regulator.
DR   PANTHER; PTHR23424; SERUM AMYLOID A; 1.
DR   PANTHER; PTHR23424:SF29; SERUM AMYLOID A PROTEIN; 1.
DR   Pfam; PF00277; SAA; 1.
DR   PIRSF; PIRSF002472; Serum_amyloid_A; 1.
DR   PRINTS; PR00306; SERUMAMYLOID.
DR   SMART; SM00197; SAA; 1.
DR   PROSITE; PS00992; SAA; 1.
PE   1: Evidence at protein level;
KW   Acute phase; Direct protein sequencing; Glycoprotein; HDL;
KW   Proteomics identification; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..18
FT   CHAIN           19..130
FT                   /note="Serum amyloid A-4 protein"
FT                   /id="PRO_0000031583"
FT   REGION          101..130
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        94
FT                   /note="N-linked (GlcNAc...) asparagine; partial"
FT                   /evidence="ECO:0000269|PubMed:16335952,
FT                   ECO:0000269|PubMed:1740433, ECO:0000269|PubMed:19159218"
FT   VARIANT         89
FT                   /note="C -> Y (confirmed at protein level;
FT                   dbSNP:rs2460827)"
FT                   /evidence="ECO:0000269|PubMed:1439582,
FT                   ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:1740433,
FT                   ECO:0000269|PubMed:22028381, ECO:0000269|PubMed:7686132,
FT                   ECO:0000269|Ref.4, ECO:0000269|Ref.6"
FT                   /id="VAR_051893"
SQ   SEQUENCE   130 AA;  14747 MW;  0F9BD50ACCF00B1C CRC64;
     MRLFTGIVFC SLVMGVTSES WRSFFKEALQ GVGDMGRAYW DIMISNHQNS NRYLYARGNY
     DAAQRGPGGV WAAKLISRSR VYLQGLIDCY LFGNSSTVLE DSKSNEKAEE WGRSGKDPDR
     FRPDGLPKKY
//