ID   CRAC_DICDI              Reviewed;         698 AA.
AC   P35401; Q54NJ7;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 4.
DT   04-NOV-2026, entry version 118.
DE   RecName: Full=Protein CRAC;
GN   Name=dagA; ORFNames=DDB_G0285161;
OS   Dictyostelium discoideum (Social amoeba).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=8089184; DOI=10.1083/jcb.126.6.1537;
RA   Insall R., Kupsa A., Lilly P.J., Shaulsky G., Levin L.R., Loomis W.F.,
RA   Devreotes P.N.;
RT   "CRAC, a cytosolic protein containing a pleckstrin homology domain, is
RT   required for receptor and G protein-mediated activation of adenylyl cyclase
RT   in Dictyostelium.";
RL   J. Cell Biol. 126:1537-1545(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   PROTEIN SEQUENCE OF 2-9.
RX   PubMed=8188693; DOI=10.1016/s0021-9258(17)36763-7;
RA   Lilly P.J., Devreotes P.N.;
RT   "Identification of CRAC, a cytosolic regulator required for guanine
RT   nucleotide stimulation of adenylyl cyclase in Dictyostelium.";
RL   J. Biol. Chem. 269:14123-14129(1994).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=9389653; DOI=10.1101/gad.11.23.3218;
RA   Chen M.-Y., Long Y., Devreotes P.N.;
RT   "A novel cytosolic regulator, Pianissimo, is required for chemoattractant
RT   receptor and G protein-mediated activation of the 12 transmembrane domain
RT   adenylyl cyclase in Dictyostelium.";
RL   Genes Dev. 11:3218-3231(1997).
CC   -!- FUNCTION: Couples activated G protein to adenylyl cyclase signal
CC       transduction from surface cAMP receptor. Pianissimo a cytosolic
CC       regulator and CRAC, are both essential for activation of the enzyme
CC       adenylyl cyclase. Pianissimo and CRAC do not function redundantly. Both
CC       proteins are integral components of the adenylyl cyclase activation
CC       pathway. {ECO:0000269|PubMed:9389653}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- DEVELOPMENTAL STAGE: Tightly developmentally regulated.
CC   -!- DISRUPTION PHENOTYPE: dagA and piaA double mutants require both
CC       proteins for reconstitution and activation of adenylyl cyclase.
CC       {ECO:0000269|PubMed:9389653}.
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DR   EMBL; U06228; AAA61782.1; -; Genomic_DNA.
DR   EMBL; AAFI02000075; EAL64837.1; -; Genomic_DNA.
DR   PIR; A54796; A54796.
DR   RefSeq; XP_638365.1; XM_633273.1.
DR   AlphaFoldDB; P35401; -.
DR   SMR; P35401; -.
DR   FunCoup; P35401; 448.
DR   STRING; 44689.P35401; -.
DR   PaxDb; 44689-P35401; -.
DR   GeneID; 8624989; -.
DR   KEGG; ddi:DDB_G0285161; -.
DR   dictyBase; DDB_G0285161; dagA.
DR   VEuPathDB; AmoebaDB:DDB_G0285161; -.
DR   eggNOG; ENOG502R7J7; Eukaryota.
DR   HOGENOM; CLU_395077_0_0_1; -.
DR   InParanoid; P35401; -.
DR   OMA; AHKHRIY; -.
DR   PRO; PR:P35401; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0031252; C:cell leading edge; IDA:dictyBase.
DR   GO; GO:0009898; C:cytoplasmic side of plasma membrane; IDA:dictyBase.
DR   GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR   GO; GO:0031256; C:leading edge membrane; IDA:dictyBase.
DR   GO; GO:0070685; C:macropinocytic cup; IDA:dictyBase.
DR   GO; GO:0016020; C:membrane; IDA:dictyBase.
DR   GO; GO:0001891; C:phagocytic cup; IDA:dictyBase.
DR   GO; GO:0005886; C:plasma membrane; IDA:dictyBase.
DR   GO; GO:0031143; C:pseudopodium; IDA:dictyBase.
DR   GO; GO:0008047; F:enzyme activator activity; IMP:dictyBase.
DR   GO; GO:0005547; F:phosphatidylinositol-3,4,5-trisphosphate binding; IDA:dictyBase.
DR   GO; GO:0043325; F:phosphatidylinositol-3,4-bisphosphate binding; IMP:dictyBase.
DR   GO; GO:0005543; F:phospholipid binding; IBA:GO_Central.
DR   GO; GO:0019887; F:protein kinase regulator activity; IMP:dictyBase.
DR   GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IDA:dictyBase.
DR   GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR   GO; GO:0006935; P:chemotaxis; IMP:dictyBase.
DR   GO; GO:0000165; P:MAPK cascade; IMP:dictyBase.
DR   GO; GO:0048015; P:phosphatidylinositol-mediated signaling; TAS:dictyBase.
DR   GO; GO:0008064; P:regulation of actin polymerization or depolymerization; IMP:dictyBase.
DR   GO; GO:0106070; P:regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway; IMP:dictyBase.
DR   GO; GO:0046688; P:response to copper ion; HDA:dictyBase.
DR   GO; GO:0030587; P:sorocarp development; HMP:dictyBase.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; TAS:dictyBase.
DR   Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR001849; PH_domain.
DR   InterPro; IPR051707; PI-Interact_SigTrans_Reg.
DR   PANTHER; PTHR14336:SF19; PROTEIN CRAC; 1.
DR   PANTHER; PTHR14336; TANDEM PH DOMAIN CONTAINING PROTEIN; 1.
DR   Pfam; PF00169; PH; 1.
DR   SMART; SM00233; PH; 1.
DR   SUPFAM; SSF50729; PH domain-like; 1.
DR   PROSITE; PS50003; PH_DOMAIN; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Developmental protein; Direct protein sequencing;
KW   Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8188693"
FT   CHAIN           2..698
FT                   /note="Protein CRAC"
FT                   /id="PRO_0000079325"
FT   DOMAIN          22..122
FT                   /note="PH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT   REGION          594..630
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        601..626
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        653
FT                   /note="R -> G (in Ref. 1; AAA61782)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   698 AA;  78630 MW;  3CC3D8FF6D9D849B CRC64;
     MGKTERKKEL LELFEYEKIK GDVSYSSIMK KAGGNGKGFL DRYFALHRNY ILYYKLGKSS
     LKPDDKQEPQ GYINLMDCNP DDTKEIAPLM FQISHKHRTY IVKAKDESSM KQFLTLLIAR
     IRSLEKIDID KLGCTVVVLT KVKKFREVLT NPLILPDRVS PEMAEEWVKQ MKNYNASFNL
     ADPFIKQVEQ ISEFCRGEVK EYIDWFGGPE GPRLAMIRCE ETVLSNWVEY INKTSSEITT
     YQDNRFFRED FKDIAVHLKN MTTFIDCYND YMIHCRKYNN NKPNTKFLEE KQTFKEYIEK
     FIPKVASCND VSLNQFYDRS LIQSSDGIVT INTSGIKKTL INQSNIISIT STTTTTTTTT
     TTTCSMPNMS NLIHSLDHTN LNIIDLNHSK SQQQLHPPPS PHHQHLHHQI VSNSKDFNIS
     VSSNNFNDGN SEFPNLDINC DFDLTSASNL SSPILSSEVP SNVVDPIGSG QGGGGSGGGG
     VTAVTEEAIN EKWHFDCNTS MIFKPPSEDG RNEGSNMSTS SITSKMSLSL NGGFDMKWVY
     QCGYFKSKNM GSISWNGKHW CWSHPRTSYK IKYIWDPTKQ SFLNIPFKSR VGATGGGSVP
     SSQSTNNLQS STSSMSSLSS SSTSTTKRSH PTTLYPDYQF KDNLLTPIII EGRHQPSLTL
     IDSPLTIPNA CLLTIAMTQY IQDALIHLSL GPKVLSSK
//