ID YOHF_ECOLI Reviewed; 253 AA. AC P33368; P33367; P76437; Q2MAU2; DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1997, sequence version 2. DT 04-NOV-2026, entry version 162. DE RecName: Full=Uncharacterized oxidoreductase YohF; DE EC=1.-.-.-; GN Name=yohF; Synonyms=yohE; OrderedLocusNames=b2137, JW2125; OS Escherichia coli (strain K12). OC Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; OC Enterobacterales; Enterobacteriaceae; Escherichia. OX NCBI_TaxID=83333; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / BHB2600; RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., RA Church G.M.; RT "Automated multiplex sequencing of the E.coli genome."; RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., RA Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1462(1997). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=16738553; DOI=10.1038/msb4100049; RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 RT and W3110."; RL Mol. Syst. Biol. 2:E1-E5(2006). CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) CC family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=AAA60499.1; Type=Frameshift; Note=Produces two separate ORFs.; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U00007; AAA60500.1; ALT_FRAME; Genomic_DNA. DR EMBL; U00007; AAA60499.1; ALT_FRAME; Genomic_DNA. DR EMBL; U00096; AAC75198.1; -; Genomic_DNA. DR EMBL; AP009048; BAE76614.1; -; Genomic_DNA. DR PIR; H64981; H64981. DR RefSeq; NP_416641.1; NC_000913.3. DR RefSeq; WP_000079538.1; NZ_CP009789.1. DR PDB; 6OZ7; X-ray; 1.36 A; A/B/C/D/E/F/G/H=2-253. DR AlphaFoldDB; P33368; -. DR SMR; P33368; -. DR BioGRID; 4260454; 23. DR DIP; DIP-12806N; -. DR FunCoup; P33368; 198. DR IntAct; P33368; 6. DR STRING; 511145.b2137; -. DR jPOST; P33368; -. DR PaxDb; 511145-b2137; -. DR EnsemblBacteria; AAC75198; AAC75198; b2137. DR GeneID; 949126; -. DR KEGG; ecj:JW2125; -. DR KEGG; eco:b2137; -. DR KEGG; ecoc:C3026_11980; -. DR PATRIC; fig|1411691.4.peg.105; -. DR EchoBASE; EB1955; -. DR eggNOG; COG1028; Bacteria. DR HOGENOM; CLU_010194_1_3_6; -. DR InParanoid; P33368; -. DR OMA; AADYMHG; -. DR OrthoDB; 286404at2; -. DR PhylomeDB; P33368; -. DR BioCyc; EcoCyc:EG12019-MONOMER; -. DR PRO; PR:P33368; -. DR Proteomes; UP000000625; Chromosome. DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central. DR FunFam; 3.40.50.720:FF:000084; Short-chain dehydrogenase reductase; 1. DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR020904; Sc_DH/Rdtase_CS. DR InterPro; IPR050259; SDR. DR InterPro; IPR002347; SDR_fam. DR NCBIfam; NF009384; PRK12743.1; 1. DR PANTHER; PTHR42879; 3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE; 1. DR PANTHER; PTHR42879:SF2; 3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG; 1. DR Pfam; PF13561; adh_short_C2; 1. DR PRINTS; PR00081; GDHRDH. DR PRINTS; PR00080; SDRFAMILY. DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1. DR PROSITE; PS00061; ADH_SHORT; 1. PE 1: Evidence at protein level; KW 3D-structure; Oxidoreductase; Reference proteome. FT CHAIN 1..253 FT /note="Uncharacterized oxidoreductase YohF" FT /id="PRO_0000054840" FT ACT_SITE 153 FT /note="Proton acceptor" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001" FT BINDING 6..30 FT /ligand="NADP(+)" FT /ligand_id="ChEBI:CHEBI:58349" FT /evidence="ECO:0000250" FT BINDING 140 FT /ligand="substrate" FT /evidence="ECO:0000250" FT STRAND 4..9 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 14..24 FT /evidence="ECO:0007829|PDB:6OZ7" FT STRAND 28..35 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 37..48 FT /evidence="ECO:0007829|PDB:6OZ7" FT TURN 49..51 FT /evidence="ECO:0007829|PDB:6OZ7" FT STRAND 54..58 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 61..63 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 66..69 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 70..78 FT /evidence="ECO:0007829|PDB:6OZ7" FT STRAND 82..86 FT /evidence="ECO:0007829|PDB:6OZ7" FT TURN 96..98 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 101..108 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 109..113 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 114..129 FT /evidence="ECO:0007829|PDB:6OZ7" FT STRAND 133..138 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 141..143 FT /evidence="ECO:0007829|PDB:6OZ7" FT STRAND 148..150 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 151..171 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 172..174 FT /evidence="ECO:0007829|PDB:6OZ7" FT STRAND 176..183 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 214..224 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 227..229 FT /evidence="ECO:0007829|PDB:6OZ7" FT STRAND 236..240 FT /evidence="ECO:0007829|PDB:6OZ7" FT HELIX 243..245 FT /evidence="ECO:0007829|PDB:6OZ7" FT TURN 248..250 FT /evidence="ECO:0007829|PDB:6OZ7" SQ SEQUENCE 253 AA; 26951 MW; B030F9EAD7E8F42A CRC64; MAQVAIITAS DSGIGKECAL LLAQQGFDIG ITWHSDEEGA KDTAREVVSH GVRAEIVQLD LGNLPEGALA LEKLIQRLGR IDVLVNNAGA MTKAPFLDMA FDEWRKIFTV DVDGAFLCSQ IAARQMVKQG QGGRIINITS VHEHTPLPDA SAYTAAKHAL GGLTKAMALE LVRHKILVNA VAPGAIATPM NGMDDSDVKP DAEPSIPLRR FGATHEIASL VVWLCSEGAN YTTGQSLIVD GGFMLANPQF NPE //