ID   ALR_PSEFL               Reviewed;          25 AA.
AC   P33162;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   09-APR-2025, entry version 65.
DE   RecName: Full=Alanine racemase;
DE            EC=5.1.1.1;
DE   Flags: Fragment;
OS   Pseudomonas fluorescens.
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Pseudomonadales; Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=294;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, COFACTOR,
RP   BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RC   STRAIN=TM5-2;
RX   PubMed=7763424; DOI=10.1271/bbb.57.93;
RA   Yokoigawa K., Kawai H., Endo K., Lim Y.H., Esaki N., Soda K.;
RT   "Thermolabile alanine racemase from a psychotroph, Pseudomonas fluorescens:
RT   purification and properties.";
RL   Biosci. Biotechnol. Biochem. 57:93-97(1993).
CC   -!- FUNCTION: Catalyzes the interconversion of L-alanine and D-alanine.
CC       {ECO:0000269|PubMed:7763424}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-alanine = D-alanine; Xref=Rhea:RHEA:20249,
CC         ChEBI:CHEBI:57416, ChEBI:CHEBI:57972; EC=5.1.1.1;
CC         Evidence={ECO:0000269|PubMed:7763424};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000269|PubMed:7763424};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8.3. {ECO:0000269|PubMed:7763424};
CC       Temperature dependence:
CC         Optimum temperature is 30 degrees Celsius. Thermolabile and
CC         psychrotrophic. Shows catalytic activity even at 0 degree Celsius.
CC         {ECO:0000269|PubMed:7763424};
CC   -!- PATHWAY: Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine
CC       from L-alanine: step 1/1.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:7763424}.
CC   -!- SIMILARITY: Belongs to the alanine racemase family. {ECO:0000305}.
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DR   PIR; PC1221; PC1221.
DR   SABIO-RK; P33162; -.
DR   UniPathway; UPA00042; UER00497.
DR   GO; GO:0008784; F:alanine racemase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030632; P:D-alanine biosynthetic process; IEA:UniProtKB-UniPathway.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Isomerase; Pyridoxal phosphate.
FT   CHAIN           1..>25
FT                   /note="Alanine racemase"
FT                   /id="PRO_0000114548"
FT   NON_TER         25
SQ   SEQUENCE   25 AA;  2775 MW;  54D51109F62B1A43 CRC64;
     MXPARALIDL QALRHNYQLA AEVIG
//