ID   SHAKB_DROME             Reviewed;         372 AA.
AC   P33085; A8JUT3; B7Z104; Q1RKZ8; Q24011; Q24520; Q26432; Q9VRB9; Q9VRC0;
AC   Q9VRC2;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 3.
DT   02-SEP-2026, entry version 175.
DE   RecName: Full=Innexin shaking-B;
DE   AltName: Full=Protein passover;
GN   Name=shakB; Synonyms=Pas, shak-B; ORFNames=CG34358;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Altocrustacea; Allotriocarida;
OC   Hexapoda; Insecta; Pterygota; Neoptera; Eumetabola; Endopterygota;
OC   Aparaglossata; Panorpida; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
RC   STRAIN=Oregon-R; TISSUE=Embryo;
RX   PubMed=1487155; DOI=10.1016/0378-1119(92)90233-f;
RA   Crompton D.E., Griffin A., Davies J.A., Miklos G.L.G.;
RT   "Analysis of a cDNA from the neurologically active locus shaking-B
RT   (Passover) of Drosophila melanogaster.";
RL   Gene 122:385-386(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NEURAL), AND DISRUPTION PHENOTYPE.
RC   STRAIN=Oregon-R; TISSUE=Pupae;
RX   PubMed=8500183; DOI=10.1016/0092-8674(93)90274-t;
RA   Krishnan S.N., Frei E., Swain G.P., Wyman R.J.;
RT   "Passover: a gene required for synaptic connectivity in the giant fiber
RT   system of Drosophila.";
RL   Cell 73:967-977(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LETHAL).
RC   TISSUE=Embryo;
RX   PubMed=7601305; DOI=10.1006/dbio.1995.1203;
RA   Crompton D., Todman M., Wilkin M., Ji S., Davies J.;
RT   "Essential and neural transcripts from the Drosophila shaking-B locus are
RT   differentially expressed in the embryonic mesoderm and pupal nervous
RT   system.";
RL   Dev. Biol. 170:142-158(1995).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LETHAL).
RX   PubMed=7892218; DOI=10.1073/pnas.92.6.2021;
RA   Krishnan S.N., Frei E., Schalet A.P., Wyman R.J.;
RT   "Molecular basis of intracistronic complementation in the Passover locus of
RT   Drosophila.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:2021-2025(1995).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [6]
RP   GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D).
RC   STRAIN=Berkeley;
RA   Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA   Park S., Wan K.H., Yu C., Celniker S.E.;
RL   Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN   [8]
RP   FUNCTION.
RX   PubMed=9552170;
RX   DOI=10.1002/(sici)1097-4695(199804)35:1<105::aid-neu9>3.0.co;2-9;
RA   Shimohigashi M., Meinertzhagen I.A.;
RT   "The shaking B gene in Drosophila regulates the number of gap junctions
RT   between photoreceptor terminals in the lamina.";
RL   J. Neurobiol. 35:105-117(1998).
RN   [9]
RP   FUNCTION, AND SUBUNIT.
RX   PubMed=9428764; DOI=10.1038/34426;
RA   Phelan P., Stebbings L.A., Baines R.A., Bacon J.P., Davies J.A., Ford C.;
RT   "Drosophila Shaking-B protein forms gap junctions in paired Xenopus
RT   oocytes.";
RL   Nature 391:181-184(1998).
RN   [10]
RP   ALTERNATIVE SPLICING.
RX   PubMed=10440730;
RX   DOI=10.1002/(sici)1097-4695(19990905)40:3<288::aid-neu2>3.0.co;2-o;
RA   Zhang Z., Curtin K.D., Sun Y.A., Wyman R.J.;
RT   "Nested transcripts of gap junction gene have distinct expression
RT   patterns.";
RL   J. Neurobiol. 40:288-301(1999).
CC   -!- FUNCTION: Structural component of the gap junctions at electrical
CC       synapses in distal and mid-depth levels in the lamina. Isoform Lethal
CC       forms voltage sensitive intercellular channels through homotypic
CC       interactions. {ECO:0000269|PubMed:9428764, ECO:0000269|PubMed:9552170}.
CC   -!- SUBUNIT: Monomer (isoform Lethal). {ECO:0000269|PubMed:9428764}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255|PROSITE-ProRule:PRU00351}. Cell junction, gap
CC       junction.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=6;
CC         Comment=Additional isoforms seem to exist.;
CC       Name=Lethal; Synonyms=A;
CC         IsoId=P33085-1; Sequence=Displayed;
CC       Name=Neural; Synonyms=C;
CC         IsoId=P33085-2; Sequence=VSP_002678, VSP_002679;
CC       Name=B;
CC         IsoId=P33085-3; Sequence=VSP_012897, VSP_012898;
CC       Name=D;
CC         IsoId=P33085-4; Sequence=VSP_036913;
CC       Name=E;
CC         IsoId=P33085-6; Sequence=VSP_037474;
CC       Name=F;
CC         IsoId=P33085-5; Sequence=VSP_036914, VSP_036915;
CC   -!- TISSUE SPECIFICITY: Isoform Neural is expressed in synapses of giant
CC       fibers (GF), in a large thoracic cell in location of postsynaptic
CC       target and optic lobe lamina and medulla. Isoform Lethal is expressed
CC       in embryonic mesodermal derivatives. During metamorphosis, both
CC       isoforms are dynamically expressed in pupal nervous system.
CC   -!- DISRUPTION PHENOTYPE: Flies fail to jump in response to a light-off
CC       stimulus. Neural-specific mutants exhibit modified giant fiber system
CC       and gustatory response. {ECO:0000269|PubMed:8500183}.
CC   -!- SIMILARITY: Belongs to the pannexin family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00351}.
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DR   EMBL; M98872; AAA89079.1; -; mRNA.
DR   EMBL; X65103; CAA46228.1; -; mRNA.
DR   EMBL; L13306; AAA28745.1; -; mRNA.
DR   EMBL; S78495; AAB34769.1; -; mRNA.
DR   EMBL; U17330; AAC46584.1; -; mRNA.
DR   EMBL; AE014298; AAF50880.2; -; Genomic_DNA.
DR   EMBL; AE014298; AAF50882.2; -; Genomic_DNA.
DR   EMBL; AE014298; AAF50883.1; -; Genomic_DNA.
DR   EMBL; AE014298; ABW09456.2; -; Genomic_DNA.
DR   EMBL; AE014298; ACL82952.1; -; Genomic_DNA.
DR   EMBL; BT025062; ABE73233.1; -; mRNA.
DR   PIR; A40734; A40734.
DR   PIR; JN0441; JN0441.
DR   RefSeq; NP_001097038.2; NM_001103568.3. [P33085-6]
DR   RefSeq; NP_001138222.1; NM_001144750.2. [P33085-5]
DR   RefSeq; NP_523425.2; NM_078701.3. [P33085-2]
DR   RefSeq; NP_608410.2; NM_134566.3. [P33085-4]
DR   RefSeq; NP_728361.1; NM_170660.2. [P33085-1]
DR   AlphaFoldDB; P33085; -.
DR   SMR; P33085; -.
DR   BioGRID; 59350; 6.
DR   FunCoup; P33085; 65.
DR   IntAct; P33085; 1.
DR   STRING; 7227.FBpp0111481; -.
DR   TCDB; 1.A.25.1.4; the gap junction-forming innexin (innexin) family.
DR   PaxDb; 7227-FBpp0111481; -.
DR   PeptideAtlas; P33085; -.
DR   EnsemblMetazoa; FBtr0112571; FBpp0111483; FBgn0085387.
DR   GeneID; 33062; -.
DR   KEGG; dme:Dmel_CG34358; -.
DR   UCSC; CG34358-RD; d. melanogaster.
DR   AGR; FB:FBgn0085387; -.
DR   CTD; 33062; -.
DR   FlyBase; FBgn0085387; shakB.
DR   VEuPathDB; VectorBase:FBgn0085387; -.
DR   eggNOG; ENOG502QWRS; Eukaryota.
DR   HOGENOM; CLU_035763_1_1_1; -.
DR   InParanoid; P33085; -.
DR   OMA; CQFFKFG; -.
DR   OrthoDB; 5867527at2759; -.
DR   PhylomeDB; P33085; -.
DR   BioGRID-ORCS; 33062; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 33062; -.
DR   PRO; PR:P33085; -.
DR   Proteomes; UP000000803; Chromosome X.
DR   Bgee; FBgn0085387; Expressed in lamina monopolar neuron L4 (Drosophila) in insect head and 214 other cell types or tissues.
DR   ExpressionAtlas; P33085; baseline and differential.
DR   GO; GO:0005921; C:gap junction; IDA:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; IDA:FlyBase.
DR   GO; GO:0005243; F:gap junction channel activity; IMP:UniProtKB.
DR   GO; GO:0009881; F:photoreceptor activity; IMP:UniProtKB.
DR   GO; GO:0010644; P:cell communication by electrical coupling; IMP:FlyBase.
DR   GO; GO:0016264; P:gap junction assembly; IMP:UniProtKB.
DR   GO; GO:0010496; P:intercellular transport; ISS:FlyBase.
DR   GO; GO:0007630; P:jump response; IMP:FlyBase.
DR   GO; GO:0007602; P:phototransduction; IMP:FlyBase.
DR   GO; GO:0003254; P:regulation of membrane depolarization; IMP:FlyBase.
DR   GO; GO:0009416; P:response to light stimulus; IMP:UniProtKB.
DR   GO; GO:0055085; P:transmembrane transport; IMP:FlyBase.
DR   InterPro; IPR000990; Innexin.
DR   PANTHER; PTHR11893; INNEXIN; 1.
DR   PANTHER; PTHR11893:SF40; INNEXIN SHAKING-B; 1.
DR   Pfam; PF00876; Innexin; 1.
DR   PRINTS; PR01262; INNEXIN.
DR   PROSITE; PS51013; PANNEXIN; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Behavior; Cell junction; Cell membrane; Gap junction;
KW   Ion channel; Ion transport; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..372
FT                   /note="Innexin shaking-B"
FT                   /id="PRO_0000208500"
FT   TOPO_DOM        1..21
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00351"
FT   TOPO_DOM        43..110
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00351"
FT   TOPO_DOM        132..182
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        183..203
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00351"
FT   TOPO_DOM        204..267
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        268..288
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00351"
FT   TOPO_DOM        289..372
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         2..119
FT                   /note="LDIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILMSFSLIITTRQYVGNPID
FT                   CVHTKDIPEDVLNTYCWIQSTYTLKSLFLKKQGVSVPYPGIGNSDGDPADKKHYKYYQW
FT                   VCFCLFF -> MGRHCHVPKQTHHIQFAQLAQSPAVCGLSESAANLRYKLLLICHRVGI
FT                   KIPRQKKIPTTYNE (in isoform E)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_037474"
FT   VAR_SEQ         2..116
FT                   /note="LDIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILMSFSLIITTRQYVGNPID
FT                   CVHTKDIPEDVLNTYCWIQSTYTLKSLFLKKQGVSVPYPGIGNSDGDPADKKHYKYYQW
FT                   VCFC -> YKPDTLTRRGSLRSLRSAPLLSTVLESTLSLTRIHPIASLELPGLDYAVHS
FT                   QSAFGAYGLAHPRDLATCTSLRSGLAAITAASASAAGGVSQSQSALLGRYGPNASIRHG
FT                   ERKIVQPKRVLSRKLKPHLVADTVKQYISRAQRTTKKGSQEQQNMEFLRGVYAFMQVSR
FT                   SSVSHVKIDSPVFRLHTNATVILLITFSIAVTTRQYVGNPIDCVHTRDIPEDVLNTYCW
FT                   IHSTYTVVDAFMKKQGSEVPFPGVHNSQGRGPLTIKHTKYYQWVAFT (in isoform
FT                   D)"
FT                   /evidence="ECO:0000303|Ref.7"
FT                   /id="VSP_036913"
FT   VAR_SEQ         2..58
FT                   /note="LDIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILMSFSLIITTRQYVGNPID
FT                   CVHTK -> VSHVKIDSPVFRLHTNATVILLITFSIAVTTRQYVGNPIDCVHTR (in
FT                   isoform Neural)"
FT                   /evidence="ECO:0000303|PubMed:8500183"
FT                   /id="VSP_002678"
FT   VAR_SEQ         2..36
FT                   /note="LDIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILM -> KKQGSEVPFPGVH
FT                   NSQGRGPLTIKHTKYYQWVAFT (in isoform F)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_036914"
FT   VAR_SEQ         37..116
FT                   /note="Missing (in isoform F)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_036915"
FT   VAR_SEQ         72..116
FT                   /note="QSTYTLKSLFLKKQGVSVPYPGIGNSDGDPADKKHYKYYQWVCFC -> HST
FT                   YTVVDAFMKKQGSEVPFPGVHNSQGRGPLTIKHTKYYQWVAFT (in isoform
FT                   Neural)"
FT                   /evidence="ECO:0000303|PubMed:8500183"
FT                   /id="VSP_002679"
FT   VAR_SEQ         121
FT                   /note="A -> P (in isoform B)"
FT                   /evidence="ECO:0000303|PubMed:1487155"
FT                   /id="VSP_012897"
FT   VAR_SEQ         123..372
FT                   /note="Missing (in isoform B)"
FT                   /evidence="ECO:0000303|PubMed:1487155"
FT                   /id="VSP_012898"
FT   CONFLICT        232
FT                   /note="I -> M (in Ref. 2; AAA28745 and 7; ABE73233)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        247..249
FT                   /note="SGE -> GEV (in Ref. 4; AAC46584)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        P33085-4:3
FT                   /note="K -> E (in Ref. 7; ABE73233)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   372 AA;  44355 MW;  3DD1049D8F1D4481 CRC64;
     MLDIFRGLKN LVKVSHVKTD SIVFRLHYSI TVMILMSFSL IITTRQYVGN PIDCVHTKDI
     PEDVLNTYCW IQSTYTLKSL FLKKQGVSVP YPGIGNSDGD PADKKHYKYY QWVCFCLFFQ
     AILFYTPRWL WKSWEGGKIH ALIMDLDIGI CSEAEKKQKK KLLLDYLWEN LRYHNWWAYR
     YYVCELLALI NVIGQMFLMN RFFDGEFITF GLKVIDYMET DQEDRMDPMI YIFPRMTKCT
     FFKYGSSGEV EKHDAICILP LNVVNEKIYI FLWFWFILLT FLTLLTLIYR VVIIFSPRMR
     VYLFRMRFRL VRRDAIEIIV RRSKMGDWFL LYLLGENIDT VIFRDVVQDL ANRLGHNQHH
     RVPGLKGEIQ DA
//