ID   TSUB_ECOLI              Reviewed;          75 AA.
AC   P33014;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   28-JAN-2026, entry version 138.
DE   RecName: Full=Putative sulfur carrier protein TsuB {ECO:0000305};
DE   AltName: Full=Thiosulfate uptake protein B {ECO:0000303|PubMed:32779574};
GN   Name=tsuB {ECO:0000303|PubMed:32779574}; Synonyms=yeeD;
GN   OrderedLocusNames=b2012, JW1994;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / BHB2600;
RA   Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA   Church G.M.;
RT   "Automated multiplex sequencing of the E.coli genome.";
RL   Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA   Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=32779574; DOI=10.2323/jgam.2019.12.004;
RA   Morigasaki S., Umeyama A., Kawano Y., Aizawa Y., Ohtsu I.;
RT   "Defect of RNA pyrophosphohydrolase RppH enhances fermentative production
RT   of L-cysteine in Escherichia coli.";
RL   J. Gen. Appl. Microbiol. 66:307-314(2021).
CC   -!- FUNCTION: Involved in thiosulfate metabolism.
CC       {ECO:0000269|PubMed:32779574}.
CC   -!- INTERACTION:
CC       P33014; P63284: clpB; NbExp=3; IntAct=EBI-9130839, EBI-546182;
CC   -!- INDUCTION: Member of the CysB regulon (PubMed:32779574). mRNA levels
CC       are increased by disruption of the rppH gene, which encodes a RNA
CC       pyrophosphohydrolase (PubMed:32779574). {ECO:0000269|PubMed:32779574}.
CC   -!- DISRUPTION PHENOTYPE: The tsuB-cysA double deletion mutant cannot grow
CC       with thiosulfate as sole sulfur source. {ECO:0000269|PubMed:32779574}.
CC   -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC       {ECO:0000305}.
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DR   EMBL; U00009; AAA16418.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75073.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15840.1; -; Genomic_DNA.
DR   PIR; C64966; C64966.
DR   RefSeq; NP_416516.1; NC_000913.3.
DR   RefSeq; WP_000234896.1; NZ_CP009789.1.
DR   AlphaFoldDB; P33014; -.
DR   SMR; P33014; -.
DR   BioGRID; 4260415; 13.
DR   BioGRID; 850886; 20.
DR   FunCoup; P33014; 11.
DR   IntAct; P33014; 20.
DR   STRING; 511145.b2012; -.
DR   jPOST; P33014; -.
DR   PaxDb; 511145-b2012; -.
DR   EnsemblBacteria; AAC75073; AAC75073; b2012.
DR   GeneID; 946539; -.
DR   KEGG; ecj:JW1994; -.
DR   KEGG; eco:b2012; -.
DR   KEGG; ecoc:C3026_11350; -.
DR   PATRIC; fig|511145.12.peg.2089; -.
DR   EchoBASE; EB1840; -.
DR   eggNOG; COG0425; Bacteria.
DR   HOGENOM; CLU_165255_0_3_6; -.
DR   InParanoid; P33014; -.
DR   OMA; SWTITVQ; -.
DR   OrthoDB; 9797352at2; -.
DR   PhylomeDB; P33014; -.
DR   BioCyc; EcoCyc:EG11894-MONOMER; -.
DR   PRO; PR:P33014; -.
DR   Proteomes; UP000000625; Chromosome.
DR   CDD; cd00291; SirA_YedF_YeeD; 1.
DR   Gene3D; 3.30.110.40; TusA-like domain; 1.
DR   InterPro; IPR001455; TusA-like.
DR   InterPro; IPR036868; TusA-like_sf.
DR   NCBIfam; NF041048; TusA_like_YeeD; 1.
DR   PANTHER; PTHR33279; SULFUR CARRIER PROTEIN YEDF-RELATED; 1.
DR   PANTHER; PTHR33279:SF6; SULFUR CARRIER PROTEIN YEDF-RELATED; 1.
DR   Pfam; PF01206; TusA; 1.
DR   SUPFAM; SSF64307; SirA-like; 1.
DR   PROSITE; PS01148; UPF0033; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..75
FT                   /note="Putative sulfur carrier protein TsuB"
FT                   /id="PRO_0000159068"
FT   ACT_SITE        13
FT                   /note="Cysteine persulfide intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:P0A890"
SQ   SEQUENCE   75 AA;  8148 MW;  5D6254BE41BEFAFA CRC64;
     MVIKKLDVVT QVCPFPLIEA KAALAEMVSG DELVIEFDCT QATEAIPQWA AEEGHAITDY
     QQIGDAAWSI TVQKA
//