ID   RIM1_YEAST              Reviewed;         135 AA.
AC   P32445; D6VR39; O11851;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   02-SEP-2026, entry version 198.
DE   RecName: Full=Single-stranded DNA-binding protein RIM1, mitochondrial;
DE   AltName: Full=Mitochondrial ssDNA-binding protein;
DE   Flags: Precursor;
GN   Name=RIM1; OrderedLocusNames=YCR028C-A; ORFNames=YCR28C-A;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=1324172; DOI=10.1002/j.1460-2075.1992.tb05421.x;
RA   van Dyck E., Foury F., Stillman B., Brill S.J.;
RT   "A single-stranded DNA binding protein required for mitochondrial DNA
RT   replication in S. cerevisiae is homologous to E. coli SSB.";
RL   EMBO J. 11:3421-3430(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   PubMed=1332309; DOI=10.1002/yea.320080915;
RA   Carbone M.L.A., Panzeri L., Falconi M.M., Carcano C., Plevani P.,
RA   Lucchini G.;
RT   "Nucleotide sequence of 9.2 kb left of CRY1 on yeast chromosome III from
RT   strain AB972: evidence for a Ty insertion and functional analysis of open
RT   reading frame YCR28.";
RL   Yeast 8:805-812(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=1574125; DOI=10.1038/357038a0;
RA   Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA   Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA   Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA   Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA   Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA   Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA   Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA   Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA   Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA   Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA   Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA   Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA   Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA   Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA   Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA   Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA   Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA   Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA   Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA   Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA   Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA   Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA   Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA   Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA   Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA   Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA   Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA   Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA   Sgouros J.G.;
RT   "The complete DNA sequence of yeast chromosome III.";
RL   Nature 357:38-46(1992).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   FUNCTION, INTERACTION WITH PIF1, AND SUBUNIT.
RX   PubMed=23175612; DOI=10.1093/nar/gks1088;
RA   Ramanagoudr-Bhojappa R., Blair L.P., Tackett A.J., Raney K.D.;
RT   "Physical and functional interaction between yeast Pif1 helicase and Rim1
RT   single-stranded DNA binding protein.";
RL   Nucleic Acids Res. 41:1029-1046(2013).
CC   -!- FUNCTION: This protein binds preferentially and cooperatively to
CC       single-stranded DNA (ssDNS). Involved in mitochondrial DNA replication.
CC       Stimulates PIF1 helicase activity. {ECO:0000269|PubMed:23175612}.
CC   -!- SUBUNIT: Homotetramer. Interacts with PIF1.
CC       {ECO:0000269|PubMed:23175612}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- MISCELLANEOUS: Present with 3060 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; S43128; AAB22978.1; -; Genomic_DNA.
DR   EMBL; S43129; AAD13828.1; -; mRNA.
DR   EMBL; X59720; CAA42321.2; -; Genomic_DNA.
DR   EMBL; BK006937; DAA07508.1; -; Genomic_DNA.
DR   PIR; S23548; S23548.
DR   RefSeq; NP_009958.2; NM_001184315.1.
DR   PDB; 6CQK; X-ray; 2.80 A; A/B/C/D=17-135.
DR   PDB; 6CQM; X-ray; 3.00 A; A/B/C/D/E/F/G/H=17-135.
DR   PDB; 6CQO; X-ray; 2.80 A; A/B/C/D/E/F/G/H=17-135.
DR   AlphaFoldDB; P32445; -.
DR   SMR; P32445; -.
DR   BioGRID; 31012; 454.
DR   DIP; DIP-7376N; -.
DR   FunCoup; P32445; 264.
DR   IntAct; P32445; 66.
DR   MINT; P32445; -.
DR   STRING; 4932.YCR028C-A; -.
DR   iPTMnet; P32445; -.
DR   PaxDb; 4932-YCR028C-A; -.
DR   PeptideAtlas; P32445; -.
DR   TopDownProteomics; P32445; -.
DR   EnsemblFungi; YCR028C-A_mRNA; YCR028C-A; YCR028C-A.
DR   GeneID; 850395; -.
DR   KEGG; sce:YCR028C-A; -.
DR   AGR; SGD:S000007222; -.
DR   SGD; S000007222; RIM1.
DR   VEuPathDB; FungiDB:YCR028C-A; -.
DR   eggNOG; ENOG502S0M8; Eukaryota.
DR   HOGENOM; CLU_126647_1_1_1; -.
DR   InParanoid; P32445; -.
DR   OMA; TQYVRKG; -.
DR   OrthoDB; 1078367at2759; -.
DR   BioCyc; YEAST:G3O-29415-MONOMER; -.
DR   Reactome; R-SCE-9837999; Mitochondrial protein degradation.
DR   BioGRID-ORCS; 850395; 9 hits in 10 CRISPR screens.
DR   PRO; PR:P32445; -.
DR   Proteomes; UP000002311; Chromosome III.
DR   RNAct; P32445; protein.
DR   GO; GO:0042645; C:mitochondrial nucleoid; IDA:SGD.
DR   GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR   GO; GO:0008047; F:enzyme activator activity; IBA:GO_Central.
DR   GO; GO:0003697; F:single-stranded DNA binding; IDA:SGD.
DR   GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR   GO; GO:0006264; P:mitochondrial DNA replication; ISS:SGD.
DR   GO; GO:0090297; P:positive regulation of mitochondrial DNA replication; IDA:SGD.
DR   CDD; cd04496; SSB_OBF; 1.
DR   FunFam; 2.40.50.140:FF:000389; RIM1p ssDNA-binding protein; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR000424; Primosome_PriB/ssb.
DR   InterPro; IPR011344; ssDNA-bd.
DR   Pfam; PF00436; SSB; 1.
DR   PIRSF; PIRSF002070; SSB; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   PROSITE; PS50935; SSB; 1.
DR   PDBsum; 6CQK; -.
DR   PDBsum; 6CQM; -.
DR   PDBsum; 6CQO; -.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; DNA replication; DNA-binding;
KW   Mitochondrion; Reference proteome; Transit peptide.
FT   TRANSIT         1..17
FT                   /note="Mitochondrion"
FT   CHAIN           18..135
FT                   /note="Single-stranded DNA-binding protein RIM1,
FT                   mitochondrial"
FT                   /id="PRO_0000033262"
FT   DOMAIN          19..117
FT                   /note="SSB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00252"
FT   STRAND          19..28
FT                   /evidence="ECO:0007829|PDB:6CQK"
FT   STRAND          33..36
FT                   /evidence="ECO:0007829|PDB:6CQK"
FT   STRAND          38..40
FT                   /evidence="ECO:0007829|PDB:6CQK"
FT   STRAND          42..50
FT                   /evidence="ECO:0007829|PDB:6CQK"
FT   STRAND          52..56
FT                   /evidence="ECO:0007829|PDB:6CQK"
FT   STRAND          59..65
FT                   /evidence="ECO:0007829|PDB:6CQK"
FT   HELIX           68..75
FT                   /evidence="ECO:0007829|PDB:6CQK"
FT   STRAND          83..93
FT                   /evidence="ECO:0007829|PDB:6CQK"
FT   STRAND          105..117
FT                   /evidence="ECO:0007829|PDB:6CQK"
SQ   SEQUENCE   135 AA;  15386 MW;  9F913C2FC9EF6E82 CRC64;
     MFLRTQARFF HATTKKMDFS KMSIVGRIGS EFTEHTSANN NRYLKYSIAS QPRRDGQTNW
     YNITVFNEPQ INFLTEYVRK GALVYVEADA ANYVFERDDG SKGTTLSLVQ KDINLLKNGK
     KLEDAEGQEN AASSE
//