ID   HMD_METWO               Reviewed;          19 AA.
AC   P32441;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   02-SEP-2026, entry version 69.
DE   RecName: Full=5,10-methenyltetrahydromethanopterin hydrogenase;
DE            EC=1.12.98.2 {ECO:0000269|PubMed:1521540};
DE   AltName: Full=H(2)-dependent methylene-H(4)MPT dehydrogenase;
DE   AltName: Full=H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase {ECO:0000303|PubMed:1521540};
DE   AltName: Full=N(5),N(10)-methenyltetrahydromethanopterin hydrogenase;
DE   AltName: Full=[Fe]-hydrogenase;
DE   Flags: Fragment;
GN   Name=hmd {ECO:0000250|UniProtKB:P32440};
OS   Methanothermobacter wolfeii (Methanobacterium wolfei).
OC   Archaea; Methanobacteriati; Methanobacteriota; Methanomada group;
OC   Methanobacteria; Methanobacteriales; Methanobacteriaceae;
OC   Methanothermobacter.
OX   NCBI_TaxID=145261;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, AND
RP   PATHWAY.
RC   STRAIN=ATCC 43096 / DSM 2970 / JCM 14652 / NBRC 100332 / VKM B-1829;
RX   PubMed=1521540; DOI=10.1111/j.1432-1033.1992.tb17215.x;
RA   Zirngibl C., van Dongen W., Schwoerer B., von Buenau R., Richter M.,
RA   Klein A., Thauer R.K.;
RT   "H2-forming methylenetetrahydromethanopterin dehydrogenase, a novel type of
RT   hydrogenase without iron-sulfur clusters in methanogenic archaea.";
RL   Eur. J. Biochem. 208:511-520(1992).
CC   -!- FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to
CC       methylene-H(4)MPT. {ECO:0000269|PubMed:1521540}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10-
CC         methylenetetrahydromethanopterin + H(+); Xref=Rhea:RHEA:20017,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:18276, ChEBI:CHEBI:57818,
CC         ChEBI:CHEBI:58337; EC=1.12.98.2;
CC         Evidence={ECO:0000269|PubMed:1521540};
CC   -!- ACTIVITY REGULATION: Very sensitive to O(2).
CC       {ECO:0000269|PubMed:1521540}.
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC       methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-
CC       tetrahydromethanopterin (hydrogen route): step 1/1.
CC       {ECO:0000269|PubMed:1521540}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:Q02394}.
CC   -!- SIMILARITY: Belongs to the HMD family. {ECO:0000305}.
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DR   AlphaFoldDB; P32441; -.
DR   STRING; 145261.MWSIV6_1534; -.
DR   BRENDA; 1.12.98.2; 3258.
DR   UniPathway; UPA00640; UER00696.
DR   GO; GO:0047068; F:N5,N10-methenyltetrahydromethanopterin hydrogenase activity; EXP:UniProtKB.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR055205; HMD_N.
DR   Pfam; PF22616; HMD_N; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Methanogenesis; One-carbon metabolism;
KW   Oxidoreductase.
FT   CHAIN           <1..19
FT                   /note="5,10-methenyltetrahydromethanopterin hydrogenase"
FT                   /id="PRO_0000218516"
FT   NON_TER         1
SQ   SEQUENCE   19 AA;  1911 MW;  0C17E9D7BF1F97C9 CRC64;
     MKLAILGAGC YRTDAASGI
//