ID   GHRHR_MOUSE             Reviewed;         423 AA.
AC   P32082; Q0VB62;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   02-SEP-2026, entry version 183.
DE   RecName: Full=Growth hormone-releasing hormone receptor;
DE            Short=GHRH receptor;
DE   AltName: Full=Growth hormone-releasing factor receptor;
DE            Short=GRF receptor;
DE            Short=GRFR;
DE   Flags: Precursor;
GN   Name=Ghrhr; Synonyms=Grfr;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1334535; DOI=10.1038/360765a0;
RA   Lin C.R., Lin S.-C., Chang C.P., Rosenfeld M.G.;
RT   "Pit-1-dependent expression of the receptor for growth hormone releasing
RT   factor mediates pituitary cell growth.";
RL   Nature 360:765-768(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   VARIANT LIT GLY-60.
RX   PubMed=8391647; DOI=10.1038/364208a0;
RA   Lin S.-C., Lin C.R., Gukovsky I., Lusis A.J., Sawchenko P.E.,
RA   Rosenfeld M.G.;
RT   "Molecular basis of the little mouse phenotype and implications for cell
RT   type-specific growth.";
RL   Nature 364:208-213(1993).
CC   -!- FUNCTION: Receptor for GRF, coupled to G proteins which activate
CC       adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone
CC       gene transcription and growth hormone secretion.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Pituitary gland.
CC   -!- DISEASE: Note=Little (lit) mice exhibits anterior pituitary hypoplasia.
CC   -!- SIMILARITY: Belongs to the G protein-coupled receptor 2 family.
CC       {ECO:0000305}.
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DR   EMBL; L07379; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; BC120748; AAI20749.1; -; mRNA.
DR   EMBL; BC120774; AAI20775.1; -; mRNA.
DR   CCDS; CCDS20165.1; -.
DR   PIR; S29753; S29753.
DR   RefSeq; NP_001003685.2; NM_001003685.3.
DR   AlphaFoldDB; P32082; -.
DR   SMR; P32082; -.
DR   BioGRID; 199917; 1.
DR   FunCoup; P32082; 401.
DR   STRING; 10090.ENSMUSP00000068120; -.
DR   GlyCosmos; P32082; 2 sites, No reported glycans.
DR   GlyGen; P32082; 2 sites.
DR   iPTMnet; P32082; -.
DR   PhosphoSitePlus; P32082; -.
DR   PaxDb; 10090-ENSMUSP00000068120; -.
DR   Antibodypedia; 3360; 310 antibodies from 33 providers.
DR   DNASU; 14602; -.
DR   Ensembl; ENSMUST00000063578.6; ENSMUSP00000068120.4; ENSMUSG00000004654.7.
DR   GeneID; 14602; -.
DR   KEGG; mmu:14602; -.
DR   UCSC; uc009cas.2; mouse.
DR   AGR; MGI:95710; -.
DR   CTD; 2692; -.
DR   MGI; MGI:95710; Ghrhr.
DR   VEuPathDB; HostDB:ENSMUSG00000004654; -.
DR   eggNOG; KOG4564; Eukaryota.
DR   GeneTree; ENSGT00940000159858; -.
DR   HOGENOM; CLU_002753_4_4_1; -.
DR   InParanoid; P32082; -.
DR   OMA; RAWGACI; -.
DR   OrthoDB; 5967113at2759; -.
DR   PhylomeDB; P32082; -.
DR   Reactome; R-MMU-418555; G alpha (s) signalling events.
DR   Reactome; R-MMU-420092; Glucagon-type ligand receptors.
DR   BioGRID-ORCS; 14602; 2 hits in 79 CRISPR screens.
DR   PRO; PR:P32082; -.
DR   Proteomes; UP000000589; Chromosome 6.
DR   RNAct; P32082; protein.
DR   Bgee; ENSMUSG00000004654; Expressed in pituitary gland and 19 other cell types or tissues.
DR   ExpressionAtlas; P32082; baseline and differential.
DR   GO; GO:0009986; C:cell surface; ISO:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; ISO:GO_Central.
DR   GO; GO:0016020; C:membrane; IDA:MGI.
DR   GO; GO:0005637; C:nuclear inner membrane; ISO:GO_Central.
DR   GO; GO:0016363; C:nuclear matrix; ISO:GO_Central.
DR   GO; GO:0005640; C:nuclear outer membrane; ISO:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR   GO; GO:0042383; C:sarcolemma; ISO:GO_Central.
DR   GO; GO:0030141; C:secretory granule; ISO:GO_Central.
DR   GO; GO:0008528; F:G protein-coupled peptide receptor activity; IBA:GO_Central.
DR   GO; GO:0019838; F:growth factor binding; ISO:GO_Central.
DR   GO; GO:0016520; F:growth hormone-releasing hormone receptor activity; IMP:MGI.
DR   GO; GO:0017046; F:peptide hormone binding; IPI:BHF-UCL.
DR   GO; GO:0007190; P:activation of adenylate cyclase activity; ISO:GO_Central.
DR   GO; GO:0021984; P:adenohypophysis development; IMP:MGI.
DR   GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IMP:MGI.
DR   GO; GO:0141156; P:cAMP/PKA signal transduction; ISO:GO_Central.
DR   GO; GO:0048469; P:cell maturation; IMP:MGI.
DR   GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR   GO; GO:0071333; P:cellular response to glucose stimulus; IEA:Ensembl.
DR   GO; GO:0032869; P:cellular response to insulin stimulus; IDA:MGI.
DR   GO; GO:0008340; P:determination of adult lifespan; IMP:MGI.
DR   GO; GO:0042445; P:hormone metabolic process; IMP:MGI.
DR   GO; GO:0007595; P:lactation; IMP:MGI.
DR   GO; GO:0030879; P:mammary gland development; IMP:MGI.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:BHF-UCL.
DR   GO; GO:0046010; P:positive regulation of circadian sleep/wake cycle, non-REM sleep; ISO:GO_Central.
DR   GO; GO:0060124; P:positive regulation of growth hormone secretion; IMP:MGI.
DR   GO; GO:0046887; P:positive regulation of hormone secretion; IMP:MGI.
DR   GO; GO:0040018; P:positive regulation of multicellular organism growth; IMP:MGI.
DR   GO; GO:0043567; P:regulation of insulin-like growth factor receptor signaling pathway; IMP:MGI.
DR   GO; GO:0033143; P:regulation of intracellular steroid hormone receptor signaling pathway; IMP:MGI.
DR   GO; GO:0051246; P:regulation of protein metabolic process; IGI:MGI.
DR   GO; GO:0043627; P:response to estrogen; ISO:GO_Central.
DR   GO; GO:0051384; P:response to glucocorticoid; ISO:GO_Central.
DR   GO; GO:0032868; P:response to insulin; ISO:GO_Central.
DR   GO; GO:0060133; P:somatotropin secreting cell development; IMP:MGI.
DR   CDD; cd15270; 7tmB1_GHRHR; 1.
DR   FunFam; 1.20.1070.10:FF:000114; Growth hormone releasing hormone receptor; 1.
DR   FunFam; 4.10.1240.10:FF:000014; Growth hormone-releasing hormone receptor 2; 1.
DR   Gene3D; 4.10.1240.10; GPCR, family 2, extracellular hormone receptor domain; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR050332; GPCR_2.
DR   InterPro; IPR017981; GPCR_2-like_7TM.
DR   InterPro; IPR036445; GPCR_2_extracell_dom_sf.
DR   InterPro; IPR001879; GPCR_2_extracellular_dom.
DR   InterPro; IPR003288; GPCR_2_GHRH_rcpt.
DR   InterPro; IPR000832; GPCR_2_secretin-like.
DR   InterPro; IPR017983; GPCR_2_secretin-like_CS.
DR   PANTHER; PTHR45620:SF14; GROWTH HORMONE-RELEASING HORMONE RECEPTOR; 1.
DR   PANTHER; PTHR45620; PDF RECEPTOR-LIKE PROTEIN-RELATED; 1.
DR   Pfam; PF00002; 7tm_2; 1.
DR   Pfam; PF02793; HRM; 1.
DR   PRINTS; PR01352; GHRHRECEPTOR.
DR   PRINTS; PR00249; GPCRSECRETIN.
DR   SMART; SM00008; HormR; 1.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   SUPFAM; SSF111418; Hormone receptor domain; 1.
DR   PROSITE; PS00649; G_PROTEIN_RECEP_F2_1; 1.
DR   PROSITE; PS00650; G_PROTEIN_RECEP_F2_2; 1.
DR   PROSITE; PS50227; G_PROTEIN_RECEP_F2_3; 1.
DR   PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Disease variant; Disulfide bond; G protein-coupled receptor;
KW   Glycoprotein; Membrane; Receptor; Reference proteome; Signal; Transducer;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..423
FT                   /note="Growth hormone-releasing hormone receptor"
FT                   /id="PRO_0000012829"
FT   TOPO_DOM        23..130
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        131..151
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        152..167
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        168..188
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        189..210
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        211..231
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        232..240
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        241..261
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        262..283
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..304
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        305..331
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        332..352
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        353..357
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        358..378
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        379..423
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        49
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        50
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        41..64
FT                   /evidence="ECO:0000250"
FT   DISULFID        55..96
FT                   /evidence="ECO:0000250"
FT   DISULFID        78..112
FT                   /evidence="ECO:0000250"
FT   VARIANT         60
FT                   /note="D -> G (in lit)"
FT                   /evidence="ECO:0000269|PubMed:8391647"
FT   CONFLICT        265..266
FT                   /note="LA -> HS (in Ref. 1; L07379)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   423 AA;  47004 MW;  A2BC658E719A8033 CRC64;
     MDGLMWATRI LCLLSLCGVT LGHLHLECDF ITQLRDDELA CLQAAEGTNN TSLGCPGTWD
     GLLCWPPTGS GQWVSLPCPE FFSHFGSDTG FVKRDCTITG WSNPFPPYPV ACPVPLELLT
     KEKSYFSTVK IIYTTGHSIS IVALCVAIAI LVALRRLHCP RNYIHTQLFA TFILKASAVF
     LKDAAIFQGD STDHCSMSTV LCKVSVAISH LATMTNFSWL LAEAVYLSCL LASTSPRSKP
     AFWWLVLAGW GLPVLCTGTW VGCKLAFEDT ECWDLDNSSP CWWIIKGPIV LSVGVNFGLF
     LNIICILLRK LEPAQGGLHT RAQYWRLSKS TLLLIPLFGI HYIIFNFLPD SAGLDIRVPL
     ELGLGSFQGF IVAVLYCFLN QEVRTEISRK WYGHDPELLP ARRTCTEWTT PPRSRLKVLT
     SEC
//