ID   SLP2_DROME              Reviewed;         451 AA.
AC   P32031; Q8SZ95; Q9VQV5;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   31-JAN-2018, sequence version 3.
DT   02-SEP-2026, entry version 179.
DE   RecName: Full=Fork head domain transcription factor slp2;
DE   AltName: Full=Sloppy paired locus protein 2;
GN   Name=slp2; ORFNames=CG2939;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Altocrustacea; Allotriocarida;
OC   Hexapoda; Insecta; Pterygota; Neoptera; Eumetabola; Endopterygota;
OC   Aparaglossata; Panorpida; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=Canton-S, and Oregon-R;
RX   PubMed=1317319; DOI=10.1101/gad.6.6.1030;
RA   Grossniklaus U., Pearson R.K., Gehring W.J.;
RT   "The Drosophila sloppy paired locus encodes two proteins involved in
RT   segmentation that show homology to mammalian transcription factors.";
RL   Genes Dev. 6:1030-1051(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley;
RA   Stapleton M., Carlson J., Frise E., Kapadia B., Park S., Wan K., Yu C.,
RA   Celniker S.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcription factor involved in segmentation. May function
CC       primarily as a segment polarity gene. Different levels of slp activity
CC       seem to be required in different segments.
CC       {ECO:0000269|PubMed:1317319}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00089}.
CC   -!- TISSUE SPECIFICITY: Expressed in the posterior half of each parasegment
CC       just anterior to the parasegmental boundary.
CC       {ECO:0000269|PubMed:1317319}.
CC   -!- DEVELOPMENTAL STAGE: Expression at 3-6 hours of embryogenesis. Strong
CC       re-expression in first-instar larvae. {ECO:0000269|PubMed:1317319}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA46891.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=CAA46892.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; X66098; CAA46892.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; X66097; CAA46891.1; ALT_FRAME; mRNA.
DR   EMBL; AE014134; AAF51057.1; -; Genomic_DNA.
DR   EMBL; AY071031; AAL48653.1; -; mRNA.
DR   EMBL; BT030401; ABO52820.1; -; mRNA.
DR   PIR; S23055; S23055.
DR   PIR; S23056; S23056.
DR   RefSeq; NP_476834.1; NM_057486.4.
DR   AlphaFoldDB; P32031; -.
DR   SMR; P32031; -.
DR   BioGRID; 59811; 7.
DR   FunCoup; P32031; 153.
DR   IntAct; P32031; 1.
DR   STRING; 7227.FBpp0077189; -.
DR   PaxDb; 7227-FBpp0077189; -.
DR   PeptideAtlas; P32031; -.
DR   DNASU; 33608; -.
DR   EnsemblMetazoa; FBtr0077500; FBpp0077189; FBgn0004567.
DR   GeneID; 33608; -.
DR   KEGG; dme:Dmel_CG2939; -.
DR   AGR; FB:FBgn0004567; -.
DR   CTD; 33608; -.
DR   FlyBase; FBgn0004567; slp2.
DR   VEuPathDB; VectorBase:FBgn0004567; -.
DR   eggNOG; KOG2294; Eukaryota.
DR   InParanoid; P32031; -.
DR   OMA; YPQFGQF; -.
DR   OrthoDB; 6230630at2759; -.
DR   Reactome; R-DME-9617828; FOXO-mediated transcription of cell cycle genes.
DR   SignaLink; P32031; -.
DR   BioGRID-ORCS; 33608; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 33608; -.
DR   PRO; PR:P32031; -.
DR   Proteomes; UP000000803; Chromosome 2L.
DR   Bgee; FBgn0004567; Expressed in lamina monopolar neuron L2 (Drosophila) in brain and 57 other cell types or tissues.
DR   ExpressionAtlas; P32031; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd20021; FH_FOXG; 1.
DR   FunFam; 1.10.10.10:FF:000135; forkhead box protein G1; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR001766; Fork_head_dom.
DR   InterPro; IPR047208; FOXG1.
DR   InterPro; IPR018122; TF_fork_head_CS_1.
DR   InterPro; IPR030456; TF_fork_head_CS_2.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR46617; FORKHEAD BOX PROTEIN G1; 1.
DR   PANTHER; PTHR46617:SF3; FORKHEAD BOX PROTEIN G1; 1.
DR   Pfam; PF00250; Forkhead; 1.
DR   PRINTS; PR00053; FORKHEAD.
DR   SMART; SM00339; FH; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR   PROSITE; PS00657; FORK_HEAD_1; 1.
DR   PROSITE; PS00658; FORK_HEAD_2; 1.
DR   PROSITE; PS50039; FORK_HEAD_3; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW   Segmentation polarity protein; Transcription; Transcription regulation.
FT   CHAIN           1..451
FT                   /note="Fork head domain transcription factor slp2"
FT                   /id="PRO_0000091915"
FT   DNA_BIND        180..274
FT                   /note="Fork-head"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT   REGION          22..42
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          73..181
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          330..369
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          402..451
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        94..104
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        111..121
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        137..161
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        162..179
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        338..366
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        419..430
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        134
FT                   /note="P -> H (in Ref. 4; AAL48653 and 5; ABO52820)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   451 AA;  49238 MW;  170C7338503B86B5 CRC64;
     MVKIEEGLPS SEISAHSLHF QHHHHPLPPT THHSALQSPH PVGLNLTNLM KMARTPHLKS
     SFSINSILPE TVEHHDEDEE EDVEKKSPAK FPPNHNNNNL NTTNWGSPED HEAESDPESD
     LDVTSMSPAP VANPNESDPD EVDEEFVEED IECDGETTDG DAENKSNDGK PVKDKKGNEK
     PPYSYNALIM MAIRQSSEKR LTLNGIYEYI MTNHPYYRDN KQGWQNSIRH NLSLNKCFVK
     VPRHYDDPGK GNYWMLDPSA EDVFIGGSTG KLRRRTTAAS RSRLAAFKRS LIGPMFPGLA
     AYPQFGQFLT YPPTAPSLLA SMYQRYNPFA PKGGPGHPGL PPGLPGLPGP PGPQGPPGPP
     PPPFVAPPTS SELYQRLQYQ QLLHQHAAAA ALAAHQRQLS VAAASAASQP PPTHHHPHLA
     VGQAPLSPGG DSPGPSPQPL HKPVTVVSRN S
//