ID STX8_YEAST Reviewed; 255 AA. AC P31377; D6VPK4; DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot. DT 16-MAY-2003, sequence version 2. DT 02-SEP-2026, entry version 179. DE RecName: Full=Syntaxin-8; DE AltName: Full=SNARE protein related to mammalian syntaxin 8; DE AltName: Full=ULP1-interacting protein 2; GN Name=SYN8; Synonyms=UIP2; OrderedLocusNames=YAL014C; ORFNames=FUN34; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND RP INTERACTION WITH PEP12; VTI1 AND SNC. RC STRAIN=ATCC 201389 / BY4742; RX PubMed=12453154; DOI=10.1034/j.1600-0854.2002.31207.x; RA Lewis M.J., Pelham H.R.B.; RT "A new yeast endosomal SNARE related to mammalian syntaxin 8."; RL Traffic 3:922-929(2002). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RA Burri L., Hofmann K., Lithgow T.; RT "Re-sequencing of the UIP2 gene reveals a frame-correction and a new Qc- RT SNARE in the endosome of Saccharomyces cerevisiae."; RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204511 / S288c / AB972; RX PubMed=8458570; DOI=10.1139/g93-005; RA Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., RA Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H.; RT "Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 RT kb region between the LTE1 and SPO7 genes."; RL Genome 36:32-42(1993). RN [4] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE. RC STRAIN=ATCC 204511 / S288c / AB972; RX PubMed=8144453; DOI=10.1128/jb.176.7.1872-1880.1994; RA Barton A.B., Kaback D.B.; RT "Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: RT analysis of the genes in the FUN38-MAK16-SPO7 region."; RL J. Bacteriol. 176:1872-1880(1994). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=7731988; DOI=10.1073/pnas.92.9.3809; RA Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., RA Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., RA Storms R.K.; RT "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."; RL Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995). RN [6] RP SEQUENCE REVISION TO C-TERMINUS. RA Dolinski K.J., Cherry J.M.; RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases. RN [7] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RX PubMed=24374639; DOI=10.1534/g3.113.008995; RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.; RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now."; RL G3 (Bethesda) 4:389-398(2014). RN [8] RP PALMITOYLATION AT CYS-238. RX PubMed=15973437; DOI=10.1038/sj.emboj.7600724; RA Valdez-Taubas J., Pelham H.R.B.; RT "Swf1-dependent palmitoylation of the SNARE Tlg1 prevents its RT ubiquitination and degradation."; RL EMBO J. 24:2524-2532(2005). RN [9] RP PALMITOYLATION. RX PubMed=16751107; DOI=10.1016/j.cell.2006.03.042; RA Roth A.F., Wan J., Bailey A.O., Sun B., Kuchar J.A., Green W.N., RA Phinney B.S., Yates J.R. III, Davis N.G.; RT "Global analysis of protein palmitoylation in yeast."; RL Cell 125:1003-1013(2006). CC -!- FUNCTION: t-SNARE which may play a role in determining the specificity CC of membrane fusion, protein transport and vesicle trafficking within CC the Golgi/endosomal and plasma membrane/endosomal systems. CC {ECO:0000269|PubMed:12453154}. CC -!- SUBUNIT: Interacts with PEP12, VTI1 and the SNC SNARE complex proteins. CC {ECO:0000269|PubMed:12453154}. CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000269|PubMed:12453154}; CC Single-pass type IV membrane protein {ECO:0000269|PubMed:12453154}. CC -!- PTM: Palmitoylated by SWF1. {ECO:0000269|PubMed:15973437, CC ECO:0000269|PubMed:16751107}. CC -!- DISRUPTION PHENOTYPE: Non-essential for growth on rich medium at 30 CC degrees Celsius (PubMed:8144453). {ECO:0000269|PubMed:8144453}. CC -!- SIMILARITY: Belongs to the syntaxin family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY205237; AAO46883.1; -; Genomic_DNA. DR EMBL; AY205236; AAO22142.1; -; Genomic_DNA. DR EMBL; L05146; AAC04943.2; -; Genomic_DNA. DR EMBL; BK006935; DAA06974.1; -; Genomic_DNA. DR PIR; S36720; S36720. DR RefSeq; NP_009388.2; NM_001178159.1. DR AlphaFoldDB; P31377; -. DR SMR; P31377; -. DR BioGRID; 31752; 114. DR ComplexPortal; CPX-5421; Endosomal SNARE complex PEP12-VTI1-SYN8-YKT6. DR ComplexPortal; CPX-5424; Endosomal SNARE complex PEP12-VTI1-SYN8-SNC1. DR ComplexPortal; CPX-5461; Endosomal SNARE complex PEP12-VTI1-SYN8-SNC2. DR DIP; DIP-5642N; -. DR FunCoup; P31377; 98. DR IntAct; P31377; 13. DR MINT; P31377; -. DR STRING; 4932.YAL014C; -. DR iPTMnet; P31377; -. DR SwissPalm; P31377; -. DR PaxDb; 4932-YAL014C; -. DR PeptideAtlas; P31377; -. DR EnsemblFungi; YAL014C_mRNA; YAL014C; YAL014C. DR GeneID; 851219; -. DR KEGG; sce:YAL014C; -. DR AGR; SGD:S000000012; -. DR SGD; S000000012; SYN8. DR VEuPathDB; FungiDB:YAL014C; -. DR eggNOG; ENOG502RZK4; Eukaryota. DR HOGENOM; CLU_053570_2_1_1; -. DR InParanoid; P31377; -. DR OMA; ELMGTRH; -. DR OrthoDB; 244190at2759; -. DR BioCyc; YEAST:G3O-28826-MONOMER; -. DR BioGRID-ORCS; 851219; 10 hits in 10 CRISPR screens. DR PRO; PR:P31377; -. DR Proteomes; UP000002311; Chromosome I. DR RNAct; P31377; protein. DR GO; GO:0005829; C:cytosol; IEA:GOC. DR GO; GO:0005768; C:endosome; IDA:SGD. DR GO; GO:0010008; C:endosome membrane; NAS:ComplexPortal. DR GO; GO:0000139; C:Golgi membrane; NAS:ComplexPortal. DR GO; GO:0031201; C:SNARE complex; NAS:ComplexPortal. DR GO; GO:0005484; F:SNAP receptor activity; IDA:SGD. DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central. DR GO; GO:0006895; P:Golgi to endosome transport; NAS:ComplexPortal. DR GO; GO:0006896; P:Golgi to vacuole transport; IMP:SGD. DR GO; GO:0048210; P:Golgi vesicle fusion to target membrane; NAS:ComplexPortal. DR GO; GO:0006886; P:intracellular protein transport; NAS:ComplexPortal. DR GO; GO:0048278; P:vesicle docking; IBA:GO_Central. DR CDD; cd15859; SNARE_SYN8; 1. DR FunFam; 1.20.5.110:FF:000060; SNARE complex subunit (Syn8); 1. DR Gene3D; 1.20.5.110; -; 1. DR InterPro; IPR062365; SYN8_N. DR InterPro; IPR000727; T_SNARE_dom. DR Pfam; PF29057; SYN8_N; 1. DR SMART; SM00397; t_SNARE; 1. DR SUPFAM; SSF58038; SNARE fusion complex; 1. DR PROSITE; PS50192; T_SNARE; 1. PE 1: Evidence at protein level; KW Coiled coil; Endosome; Lipoprotein; Membrane; Palmitate; Protein transport; KW Reference proteome; Transmembrane; Transmembrane helix; Transport. FT CHAIN 1..255 FT /note="Syntaxin-8" FT /id="PRO_0000210281" FT TOPO_DOM 1..234 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 235..255 FT /note="Helical; Anchor for type IV membrane protein" FT /evidence="ECO:0000255" FT DOMAIN 164..228 FT /note="t-SNARE coiled-coil homology" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00202" FT REGION 110..154 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 137..146 FT /note="Low complexity" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT LIPID 238 FT /note="S-palmitoyl cysteine" FT /evidence="ECO:0000305|PubMed:15973437" SQ SEQUENCE 255 AA; 29049 MW; 7631FB672F8E55D6 CRC64; MDVLKLGYEL DQLSDLVEER TRLVSVLKLA PTSNDNVTLK RQLGSILELL QKCAPNDELI SRYNTILDKI PDTAVDKELY RFQQQVARNT DEVSKESLKK VRFKNDDELT VMYKDDDEQD EESPLPSTHT PYKDEPLQSQ LQSQSQPQPP QPMVSNQELF INQQQQLLEQ DSHLGALSQS IGRTHDISLD LNNEIVSQND SLLVDLENLI DNNGRNLNRA SRSMHGFNNS RFKDNGNCVI ILVLIVVLLL LLLVL //