ID   YAFC_ECOLI              Reviewed;         304 AA.
AC   P30864;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   04-NOV-2026, entry version 165.
DE   RecName: Full=Uncharacterized HTH-type transcriptional regulator YafC;
GN   Name=yafC; OrderedLocusNames=b0208, JW0198;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RA   Nishimura K., Komine Y., Miyamoto K., Kitabatake M., Mathunaga F.,
RA   Hisano T., Miki T., Inokuchi H.;
RL   Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA   Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA   Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT   - 6.0 min (189,987 - 281,416bp) region.";
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; D12650; BAA02171.1; -; Genomic_DNA.
DR   EMBL; U70214; AAB08630.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73313.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA77879.1; -; Genomic_DNA.
DR   PIR; JS0716; JS0716.
DR   RefSeq; NP_414744.1; NC_000913.3.
DR   RefSeq; WP_000648572.1; NZ_CP009789.1.
DR   AlphaFoldDB; P30864; -.
DR   SMR; P30864; -.
DR   BioGRID; 4261788; 86.
DR   DIP; DIP-11210N; -.
DR   FunCoup; P30864; 111.
DR   IntAct; P30864; 6.
DR   STRING; 511145.b0208; -.
DR   jPOST; P30864; -.
DR   PaxDb; 511145-b0208; -.
DR   EnsemblBacteria; AAC73313; AAC73313; b0208.
DR   GeneID; 947507; -.
DR   KEGG; ecj:JW0198; -.
DR   KEGG; eco:b0208; -.
DR   KEGG; ecoc:C3026_00970; -.
DR   PATRIC; fig|1411691.4.peg.2076; -.
DR   EchoBASE; EB1602; -.
DR   eggNOG; COG0583; Bacteria.
DR   HOGENOM; CLU_039613_16_2_6; -.
DR   InParanoid; P30864; -.
DR   OMA; AAEFQWE; -.
DR   OrthoDB; 9786526at2; -.
DR   PhylomeDB; P30864; -.
DR   BioCyc; EcoCyc:EG11649-MONOMER; -.
DR   PRO; PR:P30864; -.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IBA:GO_Central.
DR   GO; GO:0010212; P:response to ionizing radiation; IMP:EcoCyc.
DR   CDD; cd08478; PBP2_CrgA; 1.
DR   FunFam; 1.10.10.10:FF:000001; LysR family transcriptional regulator; 1.
DR   FunFam; 3.40.190.10:FF:000126; Transcriptional regulator, LysR family; 1.
DR   Gene3D; 3.40.190.10; Periplasmic binding protein-like II; 2.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR048071; CrgA-like_PBP2.
DR   InterPro; IPR058163; LysR-type_TF_proteobact-type.
DR   InterPro; IPR000847; LysR_HTH_N.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   InterPro; IPR049755; YafC/CrgA.
DR   NCBIfam; NF040888; trans_reg_YafC; 1.
DR   PANTHER; PTHR30537; HTH-TYPE TRANSCRIPTIONAL REGULATOR; 1.
DR   PANTHER; PTHR30537:SF20; TRANSCRIPTIONAL REGULATORY PROTEIN; 1.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   PRINTS; PR00039; HTHLYSR.
DR   SUPFAM; SSF53850; Periplasmic binding protein-like II; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..304
FT                   /note="Uncharacterized HTH-type transcriptional regulator
FT                   YafC"
FT                   /id="PRO_0000105774"
FT   DOMAIN          1..60
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        20..39
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   304 AA;  33776 MW;  E7179C36A2264BA9 CRC64;
     MKATSEELAI FVSVVESGSF SRAAEQLGQA NSAVSRAVKK LEMKLGVSLL NRTTRQLSLT
     EEGERYFRRV QSILQEMAAA ESEIMETRNT PRGLLRIDAA TPVVLHFLMP LIKPFRERYP
     EVTLSLVSSE TIINLIERKV DVAIRAGTLT DSSLRARPLF NSYRKIIASP DYISRYGKPE
     TIDDLKQHIC LGFTEPASLN TWPIARSDGQ LHEVKYGLSS NSGETLKQLC LSGNGIACLS
     DYMIDKEIAR GELVELMADK VLPVEMPFSA VYYSDRAVST RIRAFIDFLS EHVKTAPGGA
     VREA
//