ID ACM2_CHICK Reviewed; 466 AA. AC P30372; DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot. DT 01-APR-1993, sequence version 1. DT 02-SEP-2026, entry version 158. DE RecName: Full=Muscarinic acetylcholine receptor M2; GN Name=CHRM2; Synonyms=CM2; OS Gallus gallus (Chicken). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda; OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae; OC Phasianinae; Gallus. OX NCBI_TaxID=9031; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION. RX PubMed=1840593; DOI=10.1016/s0021-9258(19)47384-5; RA Tietje K.M., Nathanson N.M.; RT "Embryonic chick heart expresses multiple muscarinic acetylcholine receptor RT subtypes. Isolation and characterization of a gene encoding a novel m2 RT muscarinic acetylcholine receptor with high affinity for pirenzepine."; RL J. Biol. Chem. 266:17382-17387(1991). CC -!- FUNCTION: Muscarinic receptor for acetylcholine, a neurotransmitter CC found in the brain, neuromuscular junctions and the autonomic ganglia CC (PubMed:1840593). Ligand binding causes a conformation change that CC triggers signaling via guanine nucleotide-binding proteins (G proteins) CC and modulates the activity of downstream effectors, such as adenylate CC cyclase (By similarity). CHRM2 is coupled to G(i)/G(o) (GNAI1 or GNAO1) CC G proteins and mediates signaling by inhibiting adenylate cyclase CC activity (By similarity). {ECO:0000250|UniProtKB:P08172, CC ECO:0000269|PubMed:1840593}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:1840593}; CC Multi-pass membrane protein {ECO:0000250|UniProtKB:P08172}. CC Postsynaptic cell membrane {ECO:0000250|UniProtKB:P08172}; Multi-pass CC membrane protein {ECO:0000250|UniProtKB:P08172}. Note=Phosphorylation CC in response to agonist binding promotes receptor internalization. CC {ECO:0000250|UniProtKB:P06199}. CC -!- MISCELLANEOUS: This receptor has a high affinity for pirenzepine. CC {ECO:0000269|PubMed:1840593}. CC -!- SIMILARITY: Belongs to the G protein-coupled receptor 1 family. CC Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily. CC {ECO:0000255|PROSITE-ProRule:PRU00521}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M73217; AAB04106.1; -; Genomic_DNA. DR PIR; A40972; A40972. DR RefSeq; NP_001025936.1; NM_001030765.2. DR RefSeq; NP_001384891.1; NM_001397962.1. DR RefSeq; XP_046795099.1; XM_046939143.1. DR RefSeq; XP_046795102.1; XM_046939146.1. DR AlphaFoldDB; P30372; -. DR SMR; P30372; -. DR FunCoup; P30372; 120. DR STRING; 9031.ENSGALP00000020999; -. DR GlyCosmos; P30372; 3 sites, No reported glycans. DR GlyGen; P30372; 3 sites. DR PaxDb; 9031-ENSGALP00000020999; -. DR Ensembl; ENSGALT00010033550.1; ENSGALP00010019773.1; ENSGALG00010013994.1. DR Ensembl; ENSGALT00010033554.1; ENSGALP00010019775.1; ENSGALG00010013994.1. DR Ensembl; ENSGALT00010033559.1; ENSGALP00010019777.1; ENSGALG00010013994.1. DR Ensembl; ENSGALT00010033565.1; ENSGALP00010019778.1; ENSGALG00010013994.1. DR Ensembl; ENSGALT00010033570.1; ENSGALP00010019779.1; ENSGALG00010013994.1. DR Ensembl; ENSGALT00010033573.1; ENSGALP00010019781.1; ENSGALG00010013994.1. DR Ensembl; ENSGALT00010033578.1; ENSGALP00010019783.1; ENSGALG00010013994.1. DR Ensembl; ENSGALT00010033586.1; ENSGALP00010019786.1; ENSGALG00010013994.1. DR Ensembl; ENSGALT00010033593.1; ENSGALP00010019788.1; ENSGALG00010013994.1. DR Ensembl; ENSGALT00010033599.1; ENSGALP00010019790.1; ENSGALG00010013994.1. DR GeneID; 418126; -. DR KEGG; gga:418126; -. DR CTD; 1129; -. DR VEuPathDB; HostDB:geneid_418126; -. DR eggNOG; KOG4220; Eukaryota. DR GeneTree; ENSGT00940000158940; -. DR HOGENOM; CLU_009579_11_2_1; -. DR InParanoid; P30372; -. DR OMA; TSERQNH; -. DR OrthoDB; 10071887at2759; -. DR PhylomeDB; P30372; -. DR Reactome; R-GGA-390648; Muscarinic acetylcholine receptors. DR Reactome; R-GGA-418594; G alpha (i) signalling events. DR Reactome; R-GGA-8856825; Cargo recognition for clathrin-mediated endocytosis. DR Reactome; R-GGA-8856828; Clathrin-mediated endocytosis. DR PRO; PR:P30372; -. DR Proteomes; UP000000539; Chromosome 1. DR Bgee; ENSGALG00000012894; Expressed in heart and 6 other cell types or tissues. DR GO; GO:0098981; C:cholinergic synapse; IEA:Ensembl. DR GO; GO:0030425; C:dendrite; IBA:GO_Central. DR GO; GO:0005886; C:plasma membrane; EXP:UniProtKB. DR GO; GO:0045211; C:postsynaptic membrane; ISS:UniProtKB. DR GO; GO:0098793; C:presynapse; IEA:GOC. DR GO; GO:0045202; C:synapse; IBA:GO_Central. DR GO; GO:1990763; F:arrestin family protein binding; IEA:Ensembl. DR GO; GO:0016907; F:G protein-coupled acetylcholine receptor activity; ISS:UniProtKB. DR GO; GO:0007197; P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway; ISS:UniProtKB. DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central. DR GO; GO:0007213; P:G protein-coupled acetylcholine receptor signaling pathway; ISS:UniProtKB. DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; IBA:GO_Central. DR GO; GO:0099171; P:presynaptic modulation of chemical synaptic transmission; IEA:Ensembl. DR GO; GO:0008016; P:regulation of heart contraction; IEA:InterPro. DR GO; GO:0006940; P:regulation of smooth muscle contraction; IBA:GO_Central. DR GO; GO:0009615; P:response to virus; IEA:Ensembl. DR CDD; cd15297; 7tmA_mAChR_M2; 1. DR FunFam; 1.20.1070.10:FF:000038; Muscarinic acetylcholine receptor; 1. DR FunFam; 1.20.1070.10:FF:000041; Muscarinic acetylcholine receptor; 1. DR Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 2. DR InterPro; IPR000276; GPCR_Rhodpsn. DR InterPro; IPR017452; GPCR_Rhodpsn_7TM. DR InterPro; IPR001065; Musac_Ach_M2_rcpt. DR InterPro; IPR000995; Musac_Ach_rcpt. DR PANTHER; PTHR24247; 5-HYDROXYTRYPTAMINE RECEPTOR; 1. DR PANTHER; PTHR24247:SF207; MUSCARINIC ACETYLCHOLINE RECEPTOR M2; 1. DR Pfam; PF00001; 7tm_1; 1. DR PRINTS; PR00237; GPCRRHODOPSN. DR PRINTS; PR00243; MUSCARINICR. DR PRINTS; PR00539; MUSCRINICM2R. DR SMART; SM01381; 7TM_GPCR_Srsx; 1. DR SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1. DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1. DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1. PE 3: Inferred from homology; KW Cell membrane; Disulfide bond; G protein-coupled receptor; Glycoprotein; KW Membrane; Postsynaptic cell membrane; Receptor; Reference proteome; KW Synapse; Transducer; Transmembrane; Transmembrane helix. FT CHAIN 1..466 FT /note="Muscarinic acetylcholine receptor M2" FT /id="PRO_0000069026" FT TOPO_DOM 1..23 FT /note="Extracellular" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TRANSMEM 24..49 FT /note="Helical; Name=1" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TOPO_DOM 50..63 FT /note="Cytoplasmic" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TRANSMEM 64..88 FT /note="Helical; Name=2" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TOPO_DOM 89..95 FT /note="Extracellular" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TRANSMEM 96..122 FT /note="Helical; Name=3" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TOPO_DOM 123..143 FT /note="Cytoplasmic" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TRANSMEM 144..165 FT /note="Helical; Name=4" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TOPO_DOM 166..185 FT /note="Extracellular" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TRANSMEM 186..210 FT /note="Helical; Name=5" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TOPO_DOM 211..387 FT /note="Cytoplasmic" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TRANSMEM 388..412 FT /note="Helical; Name=6" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TOPO_DOM 413..419 FT /note="Extracellular" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TRANSMEM 420..442 FT /note="Helical; Name=7" FT /evidence="ECO:0000250|UniProtKB:P08172" FT TOPO_DOM 443..466 FT /note="Cytoplasmic" FT /evidence="ECO:0000250|UniProtKB:P08172" FT REGION 223..265 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 279..315 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 228..238 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 246..256 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 287..298 FT /note="Low complexity" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT CARBOHYD 2 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 3 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 8 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT DISULFID 99..179 FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521" FT DISULFID 413..416 FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521" SQ SEQUENCE 466 AA; 51565 MW; 2E3D8241D6168995 CRC64; MNNSTYINSS SENVIALESP YKTIEVVFIV LVAGSLSLVT IIGNILVMVS IKVNRHLQTV NNYFLFSLAC ADLIIGIFSM NLYTLYTVIG YWPLGPVVCD LWLALDYVVS NASVMNLLII SFDRYFCVTK PLTYPVKRTT KMAGMMIAAA WVLSFILWAP AILFWQFIVG GRTVPDKDCY IQFFSNPAVT FGTAIAAFYL PVIIMTVLYW QISRASKSRI KKGKKEAAQN QDPVSPSLVQ GKIVKPNNNN IPTSSDGLEH NKVQNGKTTG ESVMENCVQG EEKDSSNDST SVSVVPSNTK EDEAAKDASQ ISASQDHLKV ENSKLTCIRI VTKSQKGDCC APTNTTVEIV GTNGDEKQNS VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW TPYNVMVLIN SFCASCIPGT VWTIGYWLCY INSTINPACY ALCNATFKKT FKHLLMCHYK NIGATR //