ID   FER_PYRFU               Reviewed;          67 AA.
AC   P29603;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   02-SEP-2026, entry version 157.
DE   RecName: Full=Ferredoxin;
GN   Name=fdxA; OrderedLocusNames=PF1909;
OS   Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC   Archaea; Methanobacteriati; Methanobacteriota; Thermococci; Thermococcales;
OC   Thermococcaceae; Pyrococcus.
OX   NCBI_TaxID=186497;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=8045914; DOI=10.1128/jb.176.15.4790-4793.1994;
RA   Heltzel A., Smith E.T., Zhou Z.H., Blamey J.M., Adams M.W.W.;
RT   "Cloning, expression, and molecular characterization of the gene encoding
RT   an extremely thermostable [4Fe-4S] ferredoxin from the hyperthermophilic
RT   archaeon Pyrococcus furiosus.";
RL   J. Bacteriol. 176:4790-4793(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA   Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA   DiRuggiero J., Robb F.T.;
RT   "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT   horikoshii inferred from complete genomic sequences.";
RL   Genetics 152:1299-1305(1999).
RN   [3]
RP   MUTAGENESIS OF ASP-15, CIRCULAR DICHROISM ANALYSIS, AND EPR SPECTROSCOPY.
RX   PubMed=9283079; DOI=10.1021/bi9708141;
RA   Zhou Z.H., Adams M.W.W.;
RT   "Site-directed mutations of the 4Fe-ferredoxin from the hyperthermophilic
RT   archaeon Pyrococcus furiosus: role of the cluster-coordinating aspartate in
RT   physiological electron transfer reactions.";
RL   Biochemistry 36:10892-10900(1997).
RN   [4]
RP   MUTAGENESIS OF ALA-34, MAGNETIC CIRCULAR DICHROISM, EPR SPECTROSCOPY, AND
RP   RESONANCE RAMAN SPECTROSCOPY OF (3FE--4S) FORM.
RA   Duderstadt R.E., Brereton P.S., Adams M.W.W., Johnson M.K.;
RT   "Spectroscopic evidence for a new type of [Fe3S4] cluster in a mutant form
RT   of Pyrococcus furiosus ferredoxin.";
RL   J. Am. Chem. Soc. 120:8525-8526(1998).
RN   [5]
RP   MUTAGENESIS OF CYSTEINE RESIDUES, MAGNETIC CIRCULAR DICHROISM, EPR
RP   SPECTROSCOPY, AND RESONANCE RAMAN SPECTROSCOPY.
RX   PubMed=10441157; DOI=10.1021/bi990671d;
RA   Brereton P.S., Duderstadt R.E., Staples C.R., Johnson M.K., Adams M.W.W.;
RT   "Effect of serinate ligation at each of the iron sites of the [Fe4S4]
RT   cluster of Pyrococcus furiosus ferredoxin on the redox, spectroscopic, and
RT   biological properties.";
RL   Biochemistry 38:10594-10605(1999).
RN   [6]
RP   MUTAGENESIS OF ALA-34, MAGNETIC CIRCULAR DICHROISM, EPR SPECTROSCOPY, AND
RP   RESONANCE RAMAN SPECTROSCOPY.
RX   PubMed=10413088; DOI=10.1016/s0014-5793(99)00766-8;
RA   Duderstadt R.E., Brereton P.S., Adams M.W.W., Johnson M.K.;
RT   "A pure S=3/2 [Fe4S4]+ cluster in the A33Y variant of Pyrococcus furiosus
RT   ferredoxin.";
RL   FEBS Lett. 454:21-26(1999).
RN   [7]
RP   STRUCTURE BY NMR.
RX   PubMed=1445925; DOI=10.1021/bi00162a038;
RA   Busse S.C., la Mar G.N., Yu L.P., Howard J.B., Smith E.T., Zhou Z.H.,
RA   Adams M.W.W.;
RT   "Proton NMR investigation of the oxidized three-iron clusters in the
RT   ferredoxins from the hyperthermophilic archae Pyrococcus furiosus and
RT   Thermococcus litoralis.";
RL   Biochemistry 31:11952-11962(1992).
RN   [8]
RP   STRUCTURE BY NMR OF 3FE--4S FORM.
RX   PubMed=8193147; DOI=10.1021/bi00186a035;
RA   Teng Q., Zhou Z.H., Smith E.T., Busse S.C., Howard J.B., Adams M.W.W.,
RA   La Mar G.N.;
RT   "Solution 1H NMR determination of secondary structure for the three-iron
RT   form of ferredoxin from the hyperthermophilic archaeon Pyrococcus
RT   furiosus.";
RL   Biochemistry 33:6316-6326(1994).
RN   [9]
RP   STRUCTURE BY NMR.
RX   PubMed=7547865; DOI=10.1021/bi00036a010;
RA   Calzolai L., Gorst C.M., Zhou Z.H., Teng Q., Adams M.W.W., La Mar G.N.;
RT   "1H NMR investigation of the electronic and molecular structure of the
RT   four-iron cluster ferredoxin from the hyperthermophile Pyrococcus furiosus.
RT   Identification of Asp 14 as a cluster ligand in each of the four redox
RT   states.";
RL   Biochemistry 34:11373-11384(1995).
RN   [10]
RP   STRUCTURE BY NMR OF 3FE--4S FORM.
RX   PubMed=7819255; DOI=10.1021/bi00002a027;
RA   Gorst C.M., Yeh Y.-H., Teng Q., Calzolai L., Zhou Z.H., Adams M.W.W.,
RA   La Mar G.N.;
RT   "1H NMR investigation of the paramagnetic cluster environment in Pyrococcus
RT   furiosus three-iron ferredoxin: sequence-specific assignment of ligated
RT   cysteines independent of tertiary structure.";
RL   Biochemistry 34:600-610(1995).
RN   [11]
RP   STRUCTURE BY NMR, AND DISULFIDE BOND.
RX   PubMed=7612619; DOI=10.1021/bi00027a030;
RA   Gorst C.M., Zhou Z.H., Teng Q., Howard J.B., Adams M.W.W., La Mar G.N.;
RT   "Participation of the disulfide bridge in the redox cycle of the ferredoxin
RT   from the hyperthermophile Pyrococcus furiosus: 1H nuclear magnetic
RT   resonance time resolution of the four redox states at ambient
RT   temperature.";
RL   Biochemistry 34:8788-8795(1995).
RN   [12]
RP   STRUCTURE BY NMR, AND MUTAGENESIS OF ASP-15.
RA   Calzolai L., Zhou Z.H., Adams M.W.W., La Mar G.N.;
RT   "Role of cluster-ligated aspartate in gating electron transfer in the four-
RT   iron ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus.";
RL   J. Am. Chem. Soc. 118:2513-2514(1996).
RN   [13]
RP   STRUCTURE BY NMR, AND MUTAGENESIS OF ASP-15.
RA   Calzolai L., Gorst C.M., Bren K.L., Zhou Z.H., Adams M.W.W., La Mar G.N.;
RT   "Solution NMR study of the electronic structure and magnetic properties of
RT   cluster ligation mutants of the four-iron ferredoxin from the
RT   hyperthermophilic archaeon Pyrococcus furiosus.";
RL   J. Am. Chem. Soc. 119:9341-9350(1997).
RN   [14]
RP   STRUCTURE BY NMR OF 3FE--4S FORM.
RX   PubMed=11601981; DOI=10.1021/bi0106179;
RA   Webba da Silva M., Sham S., Gorst C.M., Calzolai L., Brereton P.S.,
RA   Adams M.W.W., La Mar G.N.;
RT   "Solution NMR characterization of the thermodynamics of the disulfide bond
RT   orientational isomerism and its effect of cluster electronic properties for
RT   the hyperthermostable three-iron cluster ferredoxin from the archaeon
RT   Pyrococcus furiosus.";
RL   Biochemistry 40:12575-12583(2001).
RN   [15]
RP   X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR CLUSTER,
RP   AND SUBUNIT.
RX   PubMed=15122884; DOI=10.1021/bi049942x;
RA   Nielsen M.S., Harris P., Ooi B.L., Christensen H.E.;
RT   "The 1.5 A resolution crystal structure of [Fe3S4]-ferredoxin from the
RT   hyperthermophilic archaeon Pyrococcus furiosus.";
RL   Biochemistry 43:5188-5194(2004).
CC   -!- FUNCTION: Ferredoxins are iron-sulfur proteins that transfer electrons
CC       in a wide variety of metabolic reactions.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC       Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137;
CC       Note=Binds 1 [4Fe-4S] cluster which readily converts to a stable [3Fe-
CC       4S] form.;
CC   -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X79502; CAA56040.1; -; Genomic_DNA.
DR   EMBL; AE009950; AAL82033.1; -; Genomic_DNA.
DR   PIR; A44203; A44203.
DR   RefSeq; WP_011013049.1; NC_003413.1.
DR   PDB; 1SIZ; X-ray; 2.25 A; A/C=2-67.
DR   PDB; 1SJ1; X-ray; 1.50 A; A/B=2-67.
DR   PDB; 2Z8Q; X-ray; 1.70 A; A/B=2-67.
DR   PDB; 3PNI; X-ray; 2.80 A; A/B=2-67.
DR   PDB; 4DHV; X-ray; 1.95 A; A/B=2-67.
DR   AlphaFoldDB; P29603; -.
DR   SMR; P29603; -.
DR   STRING; 186497.PF1909; -.
DR   PaxDb; 186497-PF1909; -.
DR   GeneID; 13301270; -.
DR   KEGG; pfu:PF1909; -.
DR   PATRIC; fig|186497.12.peg.1980; -.
DR   eggNOG; arCOG00349; Archaea.
DR   HOGENOM; CLU_139698_6_4_2; -.
DR   OrthoDB; 5583at2157; -.
DR   PhylomeDB; P29603; -.
DR   EvolutionaryTrace; P29603; -.
DR   Proteomes; UP000001013; Chromosome.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   Gene3D; 3.30.70.20; -; 1.
DR   InterPro; IPR001080; 3Fe4S_ferredoxin.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR051269; Fe-S_cluster_ET.
DR   PANTHER; PTHR36923; FERREDOXIN; 1.
DR   PANTHER; PTHR36923:SF3; FERREDOXIN; 1.
DR   Pfam; PF13370; Fer4_13; 1.
DR   PRINTS; PR00352; 3FE4SFRDOXIN.
DR   SUPFAM; SSF54862; 4Fe-4S ferredoxins; 1.
DR   PROSITE; PS51379; 4FE4S_FER_2; 2.
DR   PDBsum; 1SIZ; -.
DR   PDBsum; 1SJ1; -.
DR   PDBsum; 2Z8Q; -.
DR   PDBsum; 3PNI; -.
DR   PDBsum; 4DHV; -.
PE   1: Evidence at protein level;
KW   3D-structure; 3Fe-4S; 4Fe-4S; Disulfide bond; Electron transport; Iron;
KW   Iron-sulfur; Metal-binding; Reference proteome; Repeat; Transport.
FT   INIT_MET        1
FT                   /note="Removed"
FT   CHAIN           2..67
FT                   /note="Ferredoxin"
FT                   /id="PRO_0000159199"
FT   DOMAIN          3..31
FT                   /note="4Fe-4S ferredoxin-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT   DOMAIN          36..67
FT                   /note="4Fe-4S ferredoxin-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT   BINDING         12
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT   BINDING         15
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT   BINDING         18
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT   BINDING         57
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT   DISULFID        22..49
FT                   /evidence="ECO:0000269|PubMed:7612619,
FT                   ECO:0000269|PubMed:7819255"
FT   MUTAGEN         15
FT                   /note="D->C: No effect on the efficiency of electron
FT                   transfer."
FT                   /evidence="ECO:0000269|PubMed:9283079, ECO:0000269|Ref.12,
FT                   ECO:0000269|Ref.13"
FT   MUTAGEN         15
FT                   /note="D->S: The efficiency of electron transfer is
FT                   compromised."
FT                   /evidence="ECO:0000269|PubMed:9283079, ECO:0000269|Ref.12,
FT                   ECO:0000269|Ref.13"
FT   MUTAGEN         34
FT                   /note="A->C: The mixed spin cluster is converted into a
FT                   homogeneous spin cluster (S=3/2)."
FT                   /evidence="ECO:0000269|PubMed:10413088, ECO:0000269|Ref.4"
FT   STRAND          3..7
FT                   /evidence="ECO:0007829|PDB:1SJ1"
FT   TURN            9..11
FT                   /evidence="ECO:0007829|PDB:1SJ1"
FT   HELIX           17..21
FT                   /evidence="ECO:0007829|PDB:1SJ1"
FT   TURN            23..25
FT                   /evidence="ECO:0007829|PDB:1SJ1"
FT   STRAND          26..28
FT                   /evidence="ECO:0007829|PDB:1SJ1"
FT   STRAND          34..37
FT                   /evidence="ECO:0007829|PDB:1SJ1"
FT   HELIX           44..56
FT                   /evidence="ECO:0007829|PDB:1SJ1"
FT   STRAND          62..66
FT                   /evidence="ECO:0007829|PDB:1SJ1"
SQ   SEQUENCE   67 AA;  7298 MW;  AF9B062690B34420 CRC64;
     MAWKVSVDQD TCIGDAICAS LCPDVFEMND EGKAQPKVEV IEDEELYNCA KEAMEACPVS
     AITIEEA
//