ID   T2H1_HAEPA              Reviewed;         254 AA.
AC   P29537;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   02-SEP-2026, entry version 61.
DE   RecName: Full=Type II restriction enzyme HpaI {ECO:0000303|PubMed:12654995};
DE            Short=R.HpaI {ECO:0000303|PubMed:1542567};
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease HpaI;
DE   AltName: Full=Type-2 restriction enzyme HpaI;
GN   Name=hpaIR;
OS   Haemophilus parainfluenzae.
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Pasteurellales; Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=729;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-22, AND FUNCTION.
RC   STRAIN=ATCC 49669;
RX   PubMed=1542567; DOI=10.1093/nar/20.4.705;
RA   Ito H., Shimato H., Sadaoka A., Kotani H., Kimizuka F., Kato I.;
RT   "Cloning and expression of the HpaI restriction-modification genes.";
RL   Nucleic Acids Res. 20:705-709(1992).
RN   [2]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-GTTAAC-3' and cleaves after T-3.
CC       {ECO:0000269|PubMed:1542567, ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
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DR   EMBL; D10668; BAA01518.1; -; Genomic_DNA.
DR   PIR; S28680; S28680.
DR   AlphaFoldDB; P29537; -.
DR   REBASE; 1158; HpaI.
DR   BRENDA; 3.1.21.4; 2532.
DR   PRO; PR:P29537; -.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   InterPro; IPR062666; T2Restr_HpaI.
DR   Pfam; PF30180; T2Restr_HpaI; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endonuclease; Hydrolase; Nuclease;
KW   Restriction system.
FT   CHAIN           1..254
FT                   /note="Type II restriction enzyme HpaI"
FT                   /id="PRO_0000077324"
SQ   SEQUENCE   254 AA;  29629 MW;  C196D22A771B9CED CRC64;
     MKYEEINFKV PVESPYYPNY SQCVIERIYS ILRNQKDMGD DRIIINTNLK KGLPLENINK
     IAGPMIEAWA EEVFSGIRDN RDNQYNLINV EAQERLGISD IILQFQVNNN VITGNVDVKA
     TSNDIPDSGK SPNITSFSRI RTAYVKDPNF IFIILSIKHS VYVKRNEYTN LMDGIMQIID
     FNVYDLKYIS DSDISYNPAL GTGQIQIKDI HYVSSQKRTT WQMCQLLDLK YLRSKKRTIE
     QFYNEAKRNK WIKD
//