ID   MMSB_PSEAE     STANDARD;      PRT;   298 AA.
AC   P28811;
DT   01-DEC-1992 (Rel. 24, Created)
DT   01-DEC-1992 (Rel. 24, Last sequence update)
DT   05-JUL-2004 (Rel. 44, Last annotation update)
DE   3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) (HIBADH).
GN   Name=mmsB; OrderedLocusNames=PA3569;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   SEQUENCE FROM N.A.
RC   STRAIN=ATCC 15692 / PAO1;
RX   MEDLINE=92317087; PubMed=1339433;
RA   Steele M.I., Lorenz D., Hatter K., Park A., Sokatch J.R.;
RT   "Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO
RT   encoding methylmalonate-semialdehyde dehydrogenase and 3-
RT   hydroxyisobutyrate dehydrogenase.";
RL   J. Biol. Chem. 267:13585-13592(1992).
RN   [2]
RP   SEQUENCE FROM N.A.
RC   STRAIN=ATCC 15692 / PAO1;
RX   MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY: 3-hydroxy-2-methylpropanoate + NAD(+) = 2-
CC       methyl-3-oxopropanoate + NADH.
CC   -!- PATHWAY: Distal valine metabolic pathway.
CC   -!- INDUCTION: By valine.
CC   -!- SIMILARITY: Belongs to the 3-hydroxyisobutyrate dehydrogenase
CC       family.
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DR   EMBL; M84911; AAA25892.1; -.
DR   EMBL; AE004778; AAG06957.1; -.
DR   PIR; C42902; C42902.
DR   InterPro; IPR002204; 3hydroxisobut_dh.
DR   InterPro; IPR006115; 6PGD_NAD.
DR   Pfam; PF03446; NAD_binding_2; 1.
DR   TIGRFAMs; TIGR01692; HIBADH; 1.
DR   PROSITE; PS00895; 3_HYDROXYISOBUT_DH; 1.
KW   Complete proteome; NAD; Oxidoreductase.
FT   NP_BIND       3     31       NAD (By similarity).
FT   ACT_SITE    171    171       By similarity.
SQ   SEQUENCE   298 AA;  30486 MW;  0C4D7B5A7C870730 CRC64;
     MTDIAFLGLG NMGGPMAANL LKAGHRVNVF DLQPKAVLGL VEQGAQGADS ALQCCEGAEV
     VISMLPAGQH VESLYLGDDG LLARVAGKPL LIDCSTIAPE TARKVAEAAA AKGLTLLDAP
     VSGGVGGARA GTLSFIVGGP AEGFARARPV LENMGRNIFH AGDHGAGQVA KICNNMLLGI
     LMAGTAEALA LGVKNGLDPA VLSEVMKQSS GGNWALNLYN PWPGVMPQAP ASNGYAGGFQ
     VRLMNKDLGL ALANAQAVQA STPLGALARN LFSLHAQADA EHEGLDFSSI QKLYRGKD
//