ID MEMA_METTR Reviewed; 526 AA. AC P27353; DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 4. DT 02-SEP-2026, entry version 119. DE RecName: Full=Methane monooxygenase component A alpha chain; DE EC=1.14.13.25; DE AltName: Full=Methane hydroxylase; GN Name=mmoX; OS Methylosinus trichosporium. OC Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria; OC Hyphomicrobiales; Methylocystaceae; Methylosinus. OX NCBI_TaxID=426; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 35070 / NCIMB 11131 / ACM 3311 / OB3b; RX PubMed=1904125; DOI=10.1111/j.1365-2958.1991.tb02114.x; RA Cardy D.L.N., Laidler V., Salmond G.P.C., Murrell J.C.; RT "Molecular analysis of the methane monooxygenase (MMO) gene cluster of RT Methylosinus trichosporium OB3b."; RL Mol. Microbiol. 5:335-342(1991). RN [2] RP SEQUENCE REVISION. RA McDonald I.; RL Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases. RN [3] RP PROTEIN SEQUENCE OF 2-16. RX PubMed=1845980; DOI=10.1016/s0021-9258(18)52470-4; RA Fox B.G., Liu Y., Dege J.E., Lipscomb J.D.; RT "Complex formation between the protein components of methane monooxygenase RT from Methylosinus trichosporium OB3b. Identification of sites of component RT interaction."; RL J. Biol. Chem. 266:540-550(1991). CC -!- FUNCTION: Responsible for the initial oxygenation of methane to CC methanol in methanotrophs. It also catalyzes the monohydroxylation of a CC variety of unactivated alkenes, alicyclic, aromatic and heterocyclic CC compounds. CC -!- CATALYTIC ACTIVITY: CC Reaction=methane + NADH + O2 + H(+) = methanol + NAD(+) + H2O; CC Xref=Rhea:RHEA:13637, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:15379, ChEBI:CHEBI:16183, ChEBI:CHEBI:17790, CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.14.13.25; CC -!- CATALYTIC ACTIVITY: CC Reaction=methane + NADPH + O2 + H(+) = methanol + NADP(+) + H2O; CC Xref=Rhea:RHEA:13641, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:15379, ChEBI:CHEBI:16183, ChEBI:CHEBI:17790, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.14.13.25; CC -!- COFACTOR: CC Name=Fe cation; Xref=ChEBI:CHEBI:24875; CC Note=Binds 2 iron ions.; CC -!- SUBUNIT: M.trichosporium has two forms of methane monooxygenase, a CC soluble and a membrane-bound type. The soluble type consists of four CC components (A to D): protein A, comprising three chains, in an alpha-2, CC beta-2, gamma-2 configuration, is a nonheme iron protein containing an CC unusual mu-hydroxo bridge structure at its active site and interacts CC with both oxygen and methane. CC -!- SIMILARITY: Belongs to the TmoA/XamoA family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X55394; CAA39068.2; -; Genomic_DNA. DR PIR; S15207; S15207. DR PDB; 1MHY; X-ray; 2.00 A; D=1-526. DR PDB; 1MHZ; X-ray; 2.70 A; D=1-526. DR PDB; 6VK4; X-ray; 2.35 A; A/E=1-526. DR PDB; 6VK5; X-ray; 1.86 A; A/E=1-526. DR PDB; 6VK6; X-ray; 1.52 A; A=1-526. DR PDB; 6VK7; X-ray; 2.12 A; A=1-526. DR PDB; 6VK8; X-ray; 2.03 A; A/E=1-526. DR PDB; 6YD0; X-ray; 1.95 A; D=1-526. DR PDB; 6YDI; X-ray; 1.95 A; D=1-526. DR PDB; 6YDU; X-ray; 1.95 A; D=1-526. DR PDB; 6YY3; X-ray; 2.00 A; D=1-526. DR PDB; 7M8Q; X-ray; 2.08 A; A/E=12-526. DR PDB; 7M8R; X-ray; 2.22 A; A/E=12-526. DR PDB; 7S6Q; X-ray; 1.96 A; A/E=12-526. DR PDB; 7S6R; X-ray; 1.89 A; A/E=12-526. DR PDB; 7S6T; X-ray; 1.82 A; A/E=12-526. DR PDB; 7S7H; X-ray; 2.40 A; A/E=12-526. DR AlphaFoldDB; P27353; -. DR SMR; P27353; -. DR BRENDA; 1.14.13.25; 3322. DR EvolutionaryTrace; P27353; -. DR GO; GO:0015049; F:methane monooxygenase [NAD(P)H] activity; IEA:UniProtKB-EC. DR CDD; cd01057; AAMH_A; 1. DR Gene3D; 1.10.620.20; Ribonucleotide Reductase, subunit A; 1. DR InterPro; IPR009078; Ferritin-like_SF. DR InterPro; IPR003430; Phenol_Hydrox. DR InterPro; IPR012348; RNR-like. DR Pfam; PF02332; Phenol_Hydrox; 1. DR SUPFAM; SSF47240; Ferritin-like; 1. DR PDBsum; 1MHY; -. DR PDBsum; 1MHZ; -. DR PDBsum; 6VK4; -. DR PDBsum; 6VK5; -. DR PDBsum; 6VK6; -. DR PDBsum; 6VK7; -. DR PDBsum; 6VK8; -. DR PDBsum; 6YD0; -. DR PDBsum; 6YDI; -. DR PDBsum; 6YDU; -. DR PDBsum; 6YY3; -. DR PDBsum; 7M8Q; -. DR PDBsum; 7M8R; -. DR PDBsum; 7S6Q; -. DR PDBsum; 7S6R; -. DR PDBsum; 7S6T; -. DR PDBsum; 7S7H; -. PE 1: Evidence at protein level; KW 3D-structure; Direct protein sequencing; Iron; Metal-binding; KW Monooxygenase; NADP; One-carbon metabolism; Oxidoreductase. FT INIT_MET 1 FT /note="Removed" FT /evidence="ECO:0000269|PubMed:1845980" FT CHAIN 2..526 FT /note="Methane monooxygenase component A alpha chain" FT /id="PRO_0000096406" FT ACT_SITE 151 FT /evidence="ECO:0000255" FT BINDING 114 FT /ligand="Fe cation" FT /ligand_id="ChEBI:CHEBI:24875" FT /ligand_label="1" FT /evidence="ECO:0000250" FT BINDING 144 FT /ligand="Fe cation" FT /ligand_id="ChEBI:CHEBI:24875" FT /ligand_label="1" FT /evidence="ECO:0000250" FT BINDING 147 FT /ligand="Fe cation" FT /ligand_id="ChEBI:CHEBI:24875" FT /ligand_label="1" FT /evidence="ECO:0000250" FT BINDING 209 FT /ligand="Fe cation" FT /ligand_id="ChEBI:CHEBI:24875" FT /ligand_label="2" FT /evidence="ECO:0000250" FT BINDING 243 FT /ligand="Fe cation" FT /ligand_id="ChEBI:CHEBI:24875" FT /ligand_label="2" FT /evidence="ECO:0000250" FT BINDING 246 FT /ligand="Fe cation" FT /ligand_id="ChEBI:CHEBI:24875" FT /ligand_label="2" FT /evidence="ECO:0000250" FT HELIX 25..29 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 32..35 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 56..58 FT /evidence="ECO:0007829|PDB:7S6R" FT HELIX 64..80 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 81..86 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 91..93 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 97..127 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 131..161 FT /evidence="ECO:0007829|PDB:6VK6" FT TURN 166..170 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 171..174 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 175..177 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 179..185 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 186..190 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 197..201 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 202..213 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 214..226 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 231..240 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 243..257 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 261..292 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 301..305 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 306..317 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 318..321 FT /evidence="ECO:0007829|PDB:6VK7" FT HELIX 322..324 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 326..329 FT /evidence="ECO:0007829|PDB:7S6T" FT HELIX 332..338 FT /evidence="ECO:0007829|PDB:6VK6" FT TURN 339..341 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 342..352 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 354..356 FT /evidence="ECO:0007829|PDB:6VK6" FT STRAND 357..360 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 366..375 FT /evidence="ECO:0007829|PDB:6VK6" FT TURN 377..379 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 380..384 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 385..392 FT /evidence="ECO:0007829|PDB:6VK6" FT TURN 393..396 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 398..400 FT /evidence="ECO:0007829|PDB:6YD0" FT HELIX 404..410 FT /evidence="ECO:0007829|PDB:6VK6" FT TURN 419..421 FT /evidence="ECO:0007829|PDB:6VK6" FT TURN 427..429 FT /evidence="ECO:0007829|PDB:6VK6" FT STRAND 437..441 FT /evidence="ECO:0007829|PDB:6VK6" FT STRAND 444..448 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 451..459 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 461..463 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 466..468 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 469..473 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 478..484 FT /evidence="ECO:0007829|PDB:6VK6" FT STRAND 492..496 FT /evidence="ECO:0007829|PDB:6VK6" FT STRAND 498..500 FT /evidence="ECO:0007829|PDB:6VK6" FT STRAND 503..505 FT /evidence="ECO:0007829|PDB:6VK6" FT HELIX 509..515 FT /evidence="ECO:0007829|PDB:6VK6" FT TURN 522..525 FT /evidence="ECO:0007829|PDB:6VK6" SQ SEQUENCE 526 AA; 59954 MW; A56F4AAD0A55726B CRC64; MAISLATKAA TDALKVNRAP VGVEPQEVHK WLQSFNWDFK ENRTKYPTKY HMANETKEQF KVIAKEYARM EAAKDERQFG TLLDGLTRLG AGNKVHPRWG ETMKVISNFL EVGEYNAIAA SAMLWDSATA AEQKNGYLAQ VLDEIRHTHQ CAFINHYYSK HYHDPAGHND ARRTRAIGPL WKGMKRVFAD GFISGDAVEC SVNLQLVGEA CFTNPLIVAV TEWASANGDE ITPTVFLSVE TDELRHMANG YQTVVSIAND PASAKFLNTD LNNAFWTQQK YFTPVLGYLF EYGSKFKVEP WVKTWNRWVY EDWGGIWIGR LGKYGVESPA SLRDAKRDAY WAHHDLALAA YAMWPLGFAR LALPDEEDQA WFEANYPGWA DHYGKIFNEW KKLGYEDPKS GFIPYQWLLA NGHDVYIDRV SQVPFIPSLA KGTGSLRVHE FNGKKHSLTD DWGERQWLIE PERYECHNVF EQYEGRELSE VIAEGHGVRS DGKTLIAQPH TRGDNLWTLE DIKRAGCVFP DPLAKF //