ID TNFB_PIG Reviewed; 204 AA. AC P26445; A5D9N9; DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1992, sequence version 1. DT 04-NOV-2026, entry version 140. DE RecName: Full=Lymphotoxin-alpha; DE Short=LT-alpha; DE AltName: Full=TNF-beta; DE AltName: Full=Tumor necrosis factor ligand superfamily member 1; DE Flags: Precursor; GN Name=LTA; Synonyms=TNFB, TNFSF1; OS Sus scrofa (Pig). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus. OX NCBI_TaxID=9823; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC TISSUE=Liver; RX PubMed=1874444; DOI=10.1016/0378-1119(91)90075-m; RA Kuhnert P., Wuethrich C., Peterhans E., Pauli U.; RT "The porcine tumor necrosis factor-encoding genes: sequence and comparative RT analysis."; RL Gene 102:171-178(1991). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Large white; TISSUE=Fibroblast; RX PubMed=11169259; DOI=10.1034/j.1399-0039.2001.057001055.x; RA Chardon P., Rogel-Gaillard C., Cattolico L., Duprat S., Vaiman M., RA Renard C.; RT "Sequence of the swine major histocompatibility complex region containing RT all non-classical class I genes."; RL Tissue Antigens 57:55-65(2001). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RG Porcine genome sequencing project; RL Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Cytokine that in its homotrimeric form binds to CC TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric CC form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by CC lymphocytes and is cytotoxic for a wide range of tumor cells in vitro CC and in vivo. {ECO:0000250|UniProtKB:P01374}. CC -!- SUBUNIT: Homotrimer, and heterotrimer of either two LTB and one LTA CC subunits or (less prevalent) two LTA and one LTB subunits. CC {ECO:0000250|UniProtKB:P01374}. CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P01374}. Cell CC membrane {ECO:0000250|UniProtKB:P01374}. Note=The homotrimer is CC secreted. The heterotrimer is membrane-associated. CC {ECO:0000250|UniProtKB:P01374}. CC -!- SIMILARITY: Belongs to the tumor necrosis factor family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X54859; CAA38638.1; -; Genomic_DNA. DR EMBL; AJ251914; CAB63853.1; -; Genomic_DNA. DR EMBL; BX548169; CAN59690.1; -; Genomic_DNA. DR PIR; S17289; S17289. DR RefSeq; NP_999618.1; NM_214453.1. DR AlphaFoldDB; P26445; -. DR SMR; P26445; -. DR FunCoup; P26445; 80. DR STRING; 9823.ENSSSCP00000028564; -. DR GlyCosmos; P26445; 1 site, No reported glycans. DR GlyGen; P26445; 1 site. DR PaxDb; 9823-ENSSSCP00000028564; -. DR Ensembl; ENSSSCT00000034506.4; ENSSSCP00000028564.1; ENSSSCG00000001403.7. DR Ensembl; ENSSSCT00000107784.1; ENSSSCP00000082172.1; ENSSSCG00000001403.7. DR Ensembl; ENSSSCT00005051223.1; ENSSSCP00005031671.1; ENSSSCG00005032113.1. DR Ensembl; ENSSSCT00005051271.1; ENSSSCP00005031708.1; ENSSSCG00005032113.1. DR Ensembl; ENSSSCT00015076476.1; ENSSSCP00015030724.1; ENSSSCG00015057385.1. DR Ensembl; ENSSSCT00015076721.1; ENSSSCP00015030833.1; ENSSSCG00015057385.1. DR Ensembl; ENSSSCT00025108214.1; ENSSSCP00025048969.1; ENSSSCG00025077752.1. DR Ensembl; ENSSSCT00025108215.1; ENSSSCP00025048970.1; ENSSSCG00025077752.1. DR Ensembl; ENSSSCT00030091914.1; ENSSSCP00030042302.1; ENSSSCG00030065787.1. DR Ensembl; ENSSSCT00030091932.1; ENSSSCP00030042313.1; ENSSSCG00030065787.1. DR Ensembl; ENSSSCT00035025316.1; ENSSSCP00035009571.1; ENSSSCG00035019536.1. DR Ensembl; ENSSSCT00040097162.1; ENSSSCP00040043299.1; ENSSSCG00040070752.1. DR Ensembl; ENSSSCT00040097211.1; ENSSSCP00040043321.1; ENSSSCG00040070752.1. DR Ensembl; ENSSSCT00045065801.1; ENSSSCP00045046587.1; ENSSSCG00045038040.1. DR Ensembl; ENSSSCT00045065841.1; ENSSSCP00045046623.1; ENSSSCG00045038040.1. DR Ensembl; ENSSSCT00050007998.1; ENSSSCP00050003405.1; ENSSSCG00050005882.1. DR Ensembl; ENSSSCT00050008003.1; ENSSSCP00050003407.1; ENSSSCG00050005882.1. DR Ensembl; ENSSSCT00055057123.1; ENSSSCP00055045693.1; ENSSSCG00055028789.1. DR Ensembl; ENSSSCT00055057155.1; ENSSSCP00055045722.1; ENSSSCG00055028789.1. DR Ensembl; ENSSSCT00060058463.1; ENSSSCP00060025023.1; ENSSSCG00060043124.1. DR Ensembl; ENSSSCT00060058470.1; ENSSSCP00060025028.1; ENSSSCG00060043124.1. DR Ensembl; ENSSSCT00065034394.1; ENSSSCP00065014269.1; ENSSSCG00065025686.1. DR Ensembl; ENSSSCT00070048168.1; ENSSSCP00070040662.1; ENSSSCG00070024123.1. DR Ensembl; ENSSSCT00070048171.1; ENSSSCP00070040664.1; ENSSSCG00070024123.1. DR Ensembl; ENSSSCT00085020783; ENSSSCP00085014258; ENSSSCG00085011147. DR GeneID; 407742; -. DR KEGG; ssc:407742; -. DR CTD; 4049; -. DR VGNC; VGNC:89881; LTA. DR eggNOG; ENOG502S4K8; Eukaryota. DR GeneTree; ENSGT01060000248544; -. DR HOGENOM; CLU_070352_4_0_1; -. DR InParanoid; P26445; -. DR OMA; ACHSGWK; -. DR OrthoDB; 9940698at2759; -. DR Reactome; R-SSC-5668541; TNFR2 non-canonical NF-kB pathway. DR Reactome; R-SSC-5669034; TNFs bind their physiological receptors. DR Reactome; R-SSC-5676594; TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway. DR Proteomes; UP000008227; Chromosome 7. DR Bgee; ENSSSCG00000001403; Expressed in blood and 11 other cell types or tissues. DR ExpressionAtlas; P26445; baseline and differential. DR GO; GO:0005576; C:extracellular region; IBA:GO_Central. DR GO; GO:0005886; C:plasma membrane; IEA:Ensembl. DR GO; GO:0002947; C:tumor necrosis factor receptor superfamily complex; IEA:Ensembl. DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central. DR GO; GO:0005164; F:tumor necrosis factor receptor binding; IEA:InterPro. DR GO; GO:0007166; P:cell surface receptor signaling pathway; IBA:GO_Central. DR GO; GO:0006959; P:humoral immune response; ISS:AgBase. DR GO; GO:0006955; P:immune response; IBA:GO_Central. DR GO; GO:0048535; P:lymph node development; ISS:AgBase. DR GO; GO:0043123; P:positive regulation of canonical NF-kappaB signal transduction; IBA:GO_Central. DR GO; GO:2001238; P:positive regulation of extrinsic apoptotic signaling pathway; IBA:GO_Central. DR CDD; cd00184; TNF; 1. DR FunFam; 2.60.120.40:FF:000016; Tumor necrosis factor; 1. DR Gene3D; 2.60.120.40; -; 1. DR InterPro; IPR006053; TNF. DR InterPro; IPR002960; TNF_beta. DR InterPro; IPR021184; TNF_CS. DR InterPro; IPR006052; TNF_dom. DR InterPro; IPR008983; Tumour_necrosis_fac-like_dom. DR PANTHER; PTHR11471:SF31; LYMPHOTOXIN-ALPHA; 1. DR PANTHER; PTHR11471; TUMOR NECROSIS FACTOR FAMILY MEMBER; 1. DR Pfam; PF00229; TNF; 1. DR PRINTS; PR01234; TNECROSISFCT. DR PRINTS; PR01236; TNFBETA. DR SMART; SM00207; TNF; 1. DR SUPFAM; SSF49842; TNF-like; 1. DR PROSITE; PS00251; THD_1; 1. DR PROSITE; PS50049; THD_2; 1. PE 3: Inferred from homology; KW Cell membrane; Cytokine; Disulfide bond; Glycoprotein; Membrane; KW Reference proteome; Secreted; Signal. FT SIGNAL 1..33 FT /evidence="ECO:0000250|UniProtKB:P01374" FT CHAIN 34..204 FT /note="Lymphotoxin-alpha" FT /id="PRO_0000034469" FT DOMAIN 62..204 FT /note="THD" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01387" FT CARBOHYD 95 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT DISULFID 119..155 FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01387" SQ SEQUENCE 204 AA; 21960 MW; 81263187435E56AC CRC64; MTPPGRLYLR RVCSTPILLL LGLLLALPPE AQGLPGVGLP PSAAQPAHQH PPKHLARGTL KPAAHLVGDP STPDSLRWRA NTDRAFLRHG FLLSNNSLLV PTSGLYFVYS QVVFSGEGCF PKATPTPLYL AHEVQLFSSQ YPFHVPLLSA QKSVCPGPQG PWVRSVYQGA VFLLTQGDQL STHTDGTPHL LLSPSSVFFG AFAL //