ID   RER1_YEAST              Reviewed;         188 AA.
AC   P25560; D6VR09;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   04-NOV-2026, entry version 169.
DE   RecName: Full=Protein RER1;
DE   AltName: Full=Retention of ER proteins 1;
GN   Name=RER1; OrderedLocusNames=YCL001W; ORFNames=YCL1W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8589449; DOI=10.1091/mbc.6.11.1459;
RA   Sato K., Nishikawa S., Nakano A.;
RT   "Membrane protein retrieval from the Golgi apparatus to the endoplasmic
RT   reticulum (ER): characterization of the RER1 gene product as a component
RT   involved in ER localization of Sec12p.";
RL   Mol. Biol. Cell 6:1459-1477(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=1574125; DOI=10.1038/357038a0;
RA   Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA   Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA   Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA   Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA   Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA   Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA   Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA   Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA   Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA   Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA   Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA   Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA   Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA   Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA   Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA   Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA   Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA   Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA   Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA   Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA   Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA   Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA   Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA   Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA   Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA   Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA   Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA   Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA   Sgouros J.G.;
RT   "The complete DNA sequence of yeast chromosome III.";
RL   Nature 357:38-46(1992).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=8367481; DOI=10.1073/pnas.90.17.8179;
RA   Nishikawa S., Nakano A.;
RT   "Identification of a gene required for membrane protein retention in the
RT   early secretory pathway.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:8179-8183(1993).
RN   [5]
RP   FUNCTION.
RX   PubMed=9275186; DOI=10.1073/pnas.94.18.9693;
RA   Sato K., Sato M., Nakano A.;
RT   "Rer1p as common machinery for the endoplasmic reticulum localization of
RT   membrane proteins.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:9693-9698(1997).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC   -!- FUNCTION: Involved in the retrieval of endoplasmic reticulum membrane
CC       proteins from the early Golgi compartment. Required for correct
CC       localization of SEC12, SEC71 and SEC63 in the endoplasmic reticulum.
CC       {ECO:0000269|PubMed:9275186}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane; Multi-pass membrane
CC       protein.
CC   -!- MISCELLANEOUS: Present with 2840 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the RER1 family. {ECO:0000305}.
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DR   EMBL; D28552; BAA05906.1; -; Genomic_DNA.
DR   EMBL; X59720; CAA42336.1; -; Genomic_DNA.
DR   EMBL; BK006937; DAA07478.1; -; Genomic_DNA.
DR   PIR; S50158; S50158.
DR   RefSeq; NP_009925.1; NM_001178651.1.
DR   AlphaFoldDB; P25560; -.
DR   BioGRID; 30977; 237.
DR   FunCoup; P25560; 976.
DR   IntAct; P25560; 18.
DR   STRING; 4932.YCL001W; -.
DR   TCDB; 9.B.82.1.1; the endoplasmic reticulum retrieval protein1 (putative heavy metal transporter) (rer1) family.
DR   iPTMnet; P25560; -.
DR   PaxDb; 4932-YCL001W; -.
DR   PeptideAtlas; P25560; -.
DR   EnsemblFungi; YCL001W_mRNA; YCL001W; YCL001W.
DR   GeneID; 850354; -.
DR   KEGG; sce:YCL001W; -.
DR   AGR; SGD:S000000507; -.
DR   SGD; S000000507; RER1.
DR   VEuPathDB; FungiDB:YCL001W; -.
DR   eggNOG; KOG1688; Eukaryota.
DR   HOGENOM; CLU_074889_0_0_1; -.
DR   InParanoid; P25560; -.
DR   OMA; AAFHQKN; -.
DR   OrthoDB; 448250at2759; -.
DR   BioCyc; YEAST:G3O-29275-MONOMER; -.
DR   BioGRID-ORCS; 850354; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P25560; -.
DR   Proteomes; UP000002311; Chromosome III.
DR   RNAct; P25560; protein.
DR   GO; GO:0030137; C:COPI-coated vesicle; IDA:SGD.
DR   GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IDA:SGD.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:GOC.
DR   GO; GO:0000324; C:fungal-type vacuole; IDA:SGD.
DR   GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR   GO; GO:0006621; P:protein retention in ER lumen; IMP:SGD.
DR   GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IDA:SGD.
DR   InterPro; IPR004932; Rer1.
DR   PANTHER; PTHR10743; PROTEIN RER1; 1.
DR   PANTHER; PTHR10743:SF0; PROTEIN RER1; 1.
DR   Pfam; PF03248; Rer1; 1.
DR   PIRSF; PIRSF016013; AtER_Rer1p; 1.
PE   1: Evidence at protein level;
KW   Golgi apparatus; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..188
FT                   /note="Protein RER1"
FT                   /id="PRO_0000207593"
FT   TOPO_DOM        1..61
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        62..82
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        83..138
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        162..188
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        109..125
FT                   /note="RPFIRRLPEFKFWYNSI -> LSIHQKTYQSSNSGITAF (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        136
FT                   /note="L -> V (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        141
FT                   /note="D -> S (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        145..188
FT                   /note="FWPILLMYFILLFFLTMRRQIQHMIKYRYIPLDIGKKKYSHSSN -> IFAH
FT                   SLFDCYFHYYCFF (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   188 AA;  22270 MW;  ACD0DAFA757C3622 CRC64;
     MDYDSSDTMN GGSSNPLITK MNTMKLLYQH YLDKVTPHAK ERWAVLGGLL CLFMVRITMA
     EGWYVICYGL GLFLLNQFLA FLTPKFDMSL QQDEENNELE AGEKSEEFRP FIRRLPEFKF
     WYNSIRATVI SLLLSLFSIF DIPVFWPILL MYFILLFFLT MRRQIQHMIK YRYIPLDIGK
     KKYSHSSN
//