ID   HRCA_BACSU              Reviewed;         343 AA.
AC   P25499;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   04-NOV-2026, entry version 146.
DE   RecName: Full=Heat-inducible transcription repressor HrcA {ECO:0000255|HAMAP-Rule:MF_00081};
GN   Name=hrcA {ECO:0000255|HAMAP-Rule:MF_00081}; Synonyms=yqeS, yqxE;
GN   OrderedLocusNames=BSU25490;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Bacillati; Bacillota; Bacilli; Caryophanales; Bacillaceae;
OC   Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / MB11;
RX   PubMed=1339421; DOI=10.1128/jb.174.10.3300-3310.1992;
RA   Wetzstein M., Voelker U., Dedio J., Loebau S., Zuber U., Schiesswohl M.,
RA   Herget C., Hecker M., Schumann W.;
RT   "Cloning, sequencing, and molecular analysis of the dnaK locus from
RT   Bacillus subtilis.";
RL   J. Bacteriol. 174:3300-3310(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT   Bacillus subtilis genome containing the skin element and many sporulation
RT   genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   FUNCTION.
RX   PubMed=8576042; DOI=10.1128/jb.178.4.1088-1093.1996;
RA   Schulz A., Schumann W.;
RT   "hrcA, the first gene of the Bacillus subtilis dnaK operon encodes a
RT   negative regulator of class I heat shock genes.";
RL   J. Bacteriol. 178:1088-1093(1996).
CC   -!- FUNCTION: Negative regulator of class I heat shock genes (grpE-dnaK-
CC       dnaJ and groELS operons). Prevents heat-shock induction of these
CC       operons. {ECO:0000255|HAMAP-Rule:MF_00081, ECO:0000269|PubMed:8576042}.
CC   -!- SIMILARITY: Belongs to the HrcA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00081}.
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DR   EMBL; M84964; AAA22526.1; -; Genomic_DNA.
DR   EMBL; D84432; BAA12462.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14491.1; -; Genomic_DNA.
DR   PIR; A41874; A41874.
DR   RefSeq; NP_390427.1; NC_000964.3.
DR   RefSeq; WP_003246126.1; NC_000964.3.
DR   AlphaFoldDB; P25499; -.
DR   SMR; P25499; -.
DR   FunCoup; P25499; 226.
DR   IntAct; P25499; 6.
DR   STRING; 224308.BSU25490; -.
DR   PaxDb; 224308-BSU25490; -.
DR   EnsemblBacteria; CAB14491; CAB14491; BSU_25490.
DR   GeneID; 937843; -.
DR   KEGG; bsu:BSU25490; -.
DR   PATRIC; fig|224308.179.peg.2770; -.
DR   eggNOG; COG1420; Bacteria.
DR   InParanoid; P25499; -.
DR   OMA; AADFTMQ; -.
DR   OrthoDB; 9783139at2; -.
DR   PhylomeDB; P25499; -.
DR   BioCyc; BSUB:BSU25490-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   AbasyAtlas; P25499; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0045892; P:negative regulation of DNA-templated transcription; IMP:CACAO.
DR   FunFam; 1.10.10.10:FF:000049; Heat-inducible transcription repressor HrcA; 1.
DR   Gene3D; 3.30.450.40; -; 1.
DR   Gene3D; 3.30.390.60; Heat-inducible transcription repressor hrca homolog, domain 3; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   HAMAP; MF_00081; HrcA; 1.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR002571; HrcA.
DR   InterPro; IPR021153; HrcA_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   InterPro; IPR023120; WHTH_transcript_rep_HrcA_IDD.
DR   NCBIfam; TIGR00331; hrcA; 1.
DR   PANTHER; PTHR34824; HEAT-INDUCIBLE TRANSCRIPTION REPRESSOR HRCA; 1.
DR   PANTHER; PTHR34824:SF1; HEAT-INDUCIBLE TRANSCRIPTION REPRESSOR HRCA; 1.
DR   Pfam; PF01628; HrcA; 1.
DR   PIRSF; PIRSF005485; HrcA; 1.
DR   SUPFAM; SSF55781; GAF domain-like; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Repressor; Stress response; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..343
FT                   /note="Heat-inducible transcription repressor HrcA"
FT                   /id="PRO_0000182449"
SQ   SEQUENCE   343 AA;  39028 MW;  0F60521FC8E30E94 CRC64;
     MLTNRQLLIL QVIINDFIKS AQPVGSRTLS KKDEITFSSA TIRNEMADLE ELGFIEKTHS
     SSGRVPSEKG YRYYVDHLLS PVKLTKSDLD QIHSIFKEKI FELEKTVQKS AQILSDLTNY
     TSIVLGPKLS ENYLKQIQII PIQPDMAVAI LVTNTGHVEN KTINFPTKMD LSDIEKLVNI
     LNDRLSGVPM DELNERIFKE VVMYLRQHIK NYDNILDALR STFHSTNHVE KLFFGGKINM
     LNQPEFHDIT RVRSLLSLIE KEQDVLKLVQ SPHTGISIKI GKENDYEEME NCSLITASYS
     VDQKQIGSIA IIGPTRMNYS RVVSLLQHVT SDLSKALTSL YDE
//