ID   CRL_ECOLI               Reviewed;         133 AA.
AC   P24251; P77629; Q47242; Q47585;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 5.
DT   02-SEP-2026, entry version 163.
DE   RecName: Full=Sigma factor-binding protein Crl;
DE   AltName: Full=Curlin genes transcriptional activatory protein;
GN   Name=crl; OrderedLocusNames=b0240, JW0230;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=6341601; DOI=10.1016/0022-2836(83)90110-9;
RA   Overbeeke N., Bergmans H., van Mansfeld F., Lugtenberg B.;
RT   "Complete nucleotide sequence of phoE, the structural gene for the
RT   phosphate limitation inducible outer membrane pore protein of Escherichia
RT   coli K12.";
RL   J. Mol. Biol. 163:513-532(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-6, AND
RP   CHARACTERIZATION.
RC   STRAIN=AO12;
RX   PubMed=2649795; DOI=10.1038/338652a0;
RA   Olsen A., Jonsson A., Normark S.;
RT   "Fibronectin binding mediated by a novel class of surface organelles on
RT   Escherichia coli.";
RL   Nature 338:652-655(1989).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=AO12;
RX   PubMed=1357528; DOI=10.1111/j.1365-2958.1992.tb01420.x;
RA   Arnqvist A., Olsen A., Pfeifer J., Russell D.G., Normark S.;
RT   "The Crl protein activates cryptic genes for curli formation and
RT   fibronectin binding in Escherichia coli HB101.";
RL   Mol. Microbiol. 6:2443-2452(1992).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA   Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA   Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT   - 6.0 min (189,987 - 281,416bp) region.";
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [7]
RP   SEQUENCE REVISION.
RC   STRAIN=K12 / MG1655 / ATCC 700926;
RX   PubMed=22081388; DOI=10.1128/jb.06087-11;
RA   Freddolino P.L., Amini S., Tavazoie S.;
RT   "Newly identified genetic variations in common Escherichia coli MG1655
RT   stock cultures.";
RL   J. Bacteriol. 194:303-306(2012).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO 28.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [9]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
RX   PubMed=6397401; DOI=10.1016/0378-1119(84)90052-0;
RA   Nueesch J., Schuemperli D.;
RT   "Structural and functional organization of the gpt gene region of
RT   Escherichia coli.";
RL   Gene 32:243-249(1984).
RN   [10]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
RC   STRAIN=O9:K30:H12;
RX   PubMed=1729226; DOI=10.1128/jb.174.1.8-16.1992;
RA   Keenleyside W.J., Jayaratne P., MacLachlan P.R., Whitfield C.;
RT   "The rcsA gene of Escherichia coli 09:K30:H12 is involved in the expression
RT   of the serotype-specific group I K (capsular) antigen.";
RL   J. Bacteriol. 174:8-16(1992).
RN   [11]
RP   ACTIVATION OF RPOS ACTIVITY.
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=9767590; DOI=10.1046/j.1365-2958.1998.01007.x;
RA   Pratt L.A., Silhavy T.J.;
RT   "Crl stimulates RpoS activity during stationary phase.";
RL   Mol. Microbiol. 29:1225-1236(1998).
RN   [12]
RP   BINDING TO SIGMA-SUBUNIT OF RNA POLYMERASE.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=14978043; DOI=10.1074/jbc.m314145200;
RA   Bougdour A., Lelong C., Geiselmann J.;
RT   "Crl, a low temperature-induced protein in Escherichia coli that binds
RT   directly to the stationary phase sigma subunit of RNA polymerase.";
RL   J. Biol. Chem. 279:19540-19550(2004).
RN   [13]
RP   ROLE IN RNA POLYMERASE HOLOENZYME FORMATION.
RX   PubMed=16980472; DOI=10.1128/jb.01266-06;
RA   Gaal T., Mandel M.J., Silhavy T.J., Gourse R.L.;
RT   "Crl facilitates RNA polymerase holoenzyme formation.";
RL   J. Bacteriol. 188:7966-7970(2006).
CC   -!- FUNCTION: Binds to the sigma-S subunit of RNA polymerase, activating
CC       expression of sigma-S-regulated genes, such as the csgBAC operon
CC       encoding the subunits of curli proteins. Stimulates RNA polymerase
CC       holoenzyme formation and may bind to several other sigma factors, such
CC       as sigma-70 (rpoD) and sigma-32 (rpoH). {ECO:0000269|PubMed:1357528,
CC       ECO:0000269|PubMed:16980472}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- INDUCTION: Induced at stationary phase, and by low temperatures.
CC   -!- SIMILARITY: Belongs to the Crl family. {ECO:0000305}.
CC   -!- CAUTION: A pseudogene in strain MG1655 / ATCC 700926, but not its
CC       parent MG1655 / ATCC 47076. In ATCC 700926 IS1I has recently inserted
CC       into the gene (PubMed:22081388). {ECO:0000305|PubMed:22081388}.
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DR   EMBL; V00316; CAA23606.1; -; Genomic_DNA.
DR   EMBL; X67207; CAA47646.1; -; Genomic_DNA.
DR   EMBL; U70214; AAB08660.1; -; Genomic_DNA.
DR   EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP009048; BAA77909.2; -; Genomic_DNA.
DR   EMBL; M13422; AAA23930.1; -; Genomic_DNA.
DR   PIR; A64749; A64749.
DR   RefSeq; WP_000174689.1; NZ_CP009789.1.
DR   PDB; 6KJ6; EM; 3.80 A; J=1-133.
DR   AlphaFoldDB; P24251; -.
DR   SMR; P24251; -.
DR   BioGRID; 4260821; 142.
DR   FunCoup; P24251; 145.
DR   IntAct; P24251; 11.
DR   jPOST; P24251; -.
DR   KEGG; ecj:JW0230; -.
DR   KEGG; ecoc:C3026_01140; -.
DR   KEGG; ecoc:C3026_23875; -.
DR   PATRIC; fig|1411691.4.peg.2043; -.
DR   EchoBASE; EB1084; -.
DR   eggNOG; ENOG502ZQ8E; Bacteria.
DR   HOGENOM; CLU_136773_0_0_6; -.
DR   InParanoid; P24251; -.
DR   OMA; FWGWWLE; -.
DR   OrthoDB; 6428303at2; -.
DR   PhylomeDB; P24251; -.
DR   PRO; PR:P24251; -.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0045893; P:positive regulation of DNA-templated transcription; IDA:EcoCyc.
DR   GO; GO:2000144; P:positive regulation of DNA-templated transcription initiation; IDA:EcoCyc.
DR   GO; GO:0065003; P:protein-containing complex assembly; IDA:EcoCyc.
DR   FunFam; 3.30.310.230:FF:000001; Sigma factor-binding protein Crl; 1.
DR   Gene3D; 3.30.310.230; Sigma factor-binding protein Crl monomer; 1.
DR   HAMAP; MF_01178; Crl; 1.
DR   InterPro; IPR009986; Tscrpt_reg_Crl.
DR   InterPro; IPR038208; Tscrpt_reg_Crl_sf.
DR   NCBIfam; NF008217; PRK10984.1; 1.
DR   Pfam; PF07417; Crl; 1.
DR   PDBsum; 6KJ6; -.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Coiled coil; Cytoplasm; Direct protein sequencing;
KW   Reference proteome; Transcription; Transcription regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:2649795"
FT   CHAIN           2..133
FT                   /note="Sigma factor-binding protein Crl"
FT                   /id="PRO_0000079355"
FT   REGION          99..122
FT                   /note="Essential for activity"
FT   COILED          90..116
FT                   /evidence="ECO:0000255"
FT   CONFLICT        8
FT                   /note="P -> L (in Ref. 1; CAA23606)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        13
FT                   /note="I -> N (in Ref. 1; CAA23606)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        29
FT                   /note="K -> E (in Ref. 2 and 3; CAA47646)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   133 AA;  15655 MW;  EA66172EF19586AA CRC64;
     MTLPSGHPKS RLIKKFTALG PYIREGKCKD NRFFFDCLAV CVNVKPAPEV REFWGWWMEL
     EAQESRFTYS YQFGLFDKAG DWKSVPVKDT EVVERLEHTL REFHEKLREL LTTLNLKLEP
     ADDFRDEPVK LTA
//