ID   MCRA_ECOLI              Reviewed;         277 AA.
AC   P24200;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   04-NOV-2026, entry version 137.
DE   RecName: Full=Type IV methyl-directed restriction enzyme EcoKMcrA {ECO:0000303|PubMed:12654995};
DE            Short=EcoKMcrA {ECO:0000303|PubMed:12654995};
DE            EC=3.1.21.-;
DE   AltName: Full=5-methylcytosine-specific restriction enzyme A;
GN   Name=mcrA {ECO:0000303|PubMed:1938927}; Synonyms=rglA;
GN   OrderedLocusNames=b1159, JW1145;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1938927; DOI=10.1128/jb.173.22.7368-7373.1991;
RA   Hiom K.J., Sedgwick S.G.;
RT   "Cloning and structural characterization of the mcrA locus of Escherichia
RT   coli.";
RL   J. Bacteriol. 173:7368-7373(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   DOI=10.1007/BF02703150;
RA   Ramalingam R., Prasad R., Shivapriya R., Dharmalingam K.;
RT   "Molecular cloning and sequencing of mcrA locus and identification of McrA
RT   protein in Escherichia coli.";
RL   J. Biosci. 17:217-232(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   NOMENCLATURE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: Restriction of 5-methyl and 5-hydroxymethylcytosines at the
CC       specific DNA sequence 5'-C(me)CGG-3'.
CC   -!- MISCELLANEOUS: Encoded by the SOS-inducible cryptic lambdoid prophage
CC       e14 (PubMed:16738553, PubMed:1938927, PubMed:9278503, Ref.2). UV
CC       treatment leads to prophage excision and reduced levels of McrA
CC       (PubMed:1938927, Ref.2). {ECO:0000269|PubMed:16738553,
CC       ECO:0000269|PubMed:1938927, ECO:0000269|PubMed:9278503,
CC       ECO:0000269|Ref.2}.
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DR   EMBL; M76667; AAA68481.1; -; Genomic_DNA.
DR   EMBL; Z19104; CAA79520.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74243.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35995.1; -; Genomic_DNA.
DR   PIR; A41424; A41424.
DR   RefSeq; NP_415677.1; NC_000913.3.
DR   RefSeq; WP_000557907.1; NZ_CP010438.1.
DR   PDB; 6GHC; X-ray; 2.85 A; A/B=1-277.
DR   PDB; 6R64; X-ray; 2.64 A; A/B=1-143.
DR   PDB; 6T21; X-ray; 2.07 A; A/B=1-143.
DR   PDB; 6T22; X-ray; 2.21 A; A/B=1-143.
DR   SASBDB; P24200; -.
DR   SMR; P24200; -.
DR   BioGRID; 4262863; 150.
DR   IntAct; P24200; 3.
DR   STRING; 511145.b1159; -.
DR   REBASE; 13372; EcoW3110McrAP.
DR   REBASE; 155511; VscVS05ORF515P.
DR   REBASE; 2832; EcoKMcrA.
DR   PaxDb; 511145-b1159; -.
DR   EnsemblBacteria; AAC74243; AAC74243; b1159.
DR   GeneID; 945727; -.
DR   KEGG; ecj:JW1145; -.
DR   KEGG; eco:b1159; -.
DR   PATRIC; fig|511145.12.peg.1200; -.
DR   EchoBASE; EB0568; -.
DR   eggNOG; COG1403; Bacteria.
DR   HOGENOM; CLU_1003791_0_0_6; -.
DR   InParanoid; P24200; -.
DR   BioCyc; EcoCyc:EG10573-MONOMER; -.
DR   BioCyc; MetaCyc:EG10573-MONOMER; -.
DR   PRO; PR:P24200; -.
DR   CDD; cd00085; HNHc; 1.
DR   Gene3D; 1.10.30.50; -; 1.
DR   InterPro; IPR002711; HNH.
DR   InterPro; IPR003615; HNH_nuc.
DR   InterPro; IPR062342; McrA_N.
DR   PANTHER; PTHR33877; SLL1193 PROTEIN; 1.
DR   PANTHER; PTHR33877:SF1; TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMCRA; 1.
DR   Pfam; PF01844; HNH; 1.
DR   Pfam; PF30443; McrA_N; 1.
DR   SMART; SM00507; HNHc; 1.
DR   GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR   GO; GO:0008327; F:methyl-CpG binding; IDA:EcoliWiki.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   Proteomes; UP000000625; Chromosome.
DR   AlphaFoldDB; P24200; -.
DR   FunCoup; P24200; 7.
PE   1: Evidence at protein level;
KW   3D-structure; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   Restriction system.
FT   CHAIN           1..277
FT                   /note="Type IV methyl-directed restriction enzyme EcoKMcrA"
FT                   /id="PRO_0000077380"
FT   DOMAIN          207..257
FT                   /note="HNH"
FT   STRAND          1..4
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   STRAND          10..20
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   STRAND          23..28
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   TURN            34..39
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   HELIX           40..53
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   STRAND          58..63
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   HELIX           66..71
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   HELIX           75..78
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   STRAND          79..84
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   HELIX           93..102
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   HELIX           103..106
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   STRAND          109..112
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   STRAND          124..127
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   HELIX           134..142
FT                   /evidence="ECO:0007829|PDB:6T21"
FT   HELIX           145..148
FT                   /evidence="ECO:0007829|PDB:6GHC"
FT   HELIX           155..167
FT                   /evidence="ECO:0007829|PDB:6GHC"
FT   STRAND          176..178
FT                   /evidence="ECO:0007829|PDB:6GHC"
FT   STRAND          181..189
FT                   /evidence="ECO:0007829|PDB:6GHC"
FT   HELIX           193..203
FT                   /evidence="ECO:0007829|PDB:6GHC"
FT   STRAND          208..210
FT                   /evidence="ECO:0007829|PDB:6GHC"
FT   STRAND          215..218
FT                   /evidence="ECO:0007829|PDB:6GHC"
FT   TURN            219..221
FT                   /evidence="ECO:0007829|PDB:6GHC"
FT   STRAND          226..231
FT                   /evidence="ECO:0007829|PDB:6GHC"
FT   TURN            233..236
FT                   /evidence="ECO:0007829|PDB:6GHC"
FT   HELIX           241..243
FT                   /evidence="ECO:0007829|PDB:6GHC"
FT   STRAND          244..247
FT                   /evidence="ECO:0007829|PDB:6GHC"
FT   HELIX           249..257
FT                   /evidence="ECO:0007829|PDB:6GHC"
FT   HELIX           261..271
FT                   /evidence="ECO:0007829|PDB:6GHC"
SQ   SEQUENCE   277 AA;  31390 MW;  E5F2627DFFDEC402 CRC64;
     MHVFDNNGIE LKAECSIGEE DGVYGLILES WGPGDRNKDY NIALDYIIER LVDSGVSQVV
     VYLASSSVRK HMHSLDERKI HPGEYFTLIG NSPRDIRLKM CGYQAYFSRT GRKEIPSGNR
     TKRILINVPG IYSDSFWASI IRGELSELSQ PTDDESLLNM RVSKLIKKTL SQPEGSRKPV
     EVERLQKVYV RDPMVKAWIL QQSKGICENC GKNAPFYLND GNPYLEVHHV IPLSSGGADT
     TDNCVALCPN CHRELHYSKN AKELIEMLYV NINRLQK
//