ID   XYLG_PSEPU              Reviewed;         486 AA.
AC   P23105;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   02-SEP-2026, entry version 118.
DE   RecName: Full=2-hydroxymuconic semialdehyde dehydrogenase;
DE            Short=HMSD;
DE            EC=1.2.1.85;
GN   Name=xylG;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid TOL pWW0.
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Pseudomonadales; Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1791759; DOI=10.1111/j.1365-2958.1991.tb02091.x;
RA   Horn J.M., Harayama S., Timmis K.N.;
RT   "DNA sequence determination of the TOL plasmid (pWWO) xylGFJ genes of
RT   Pseudomonas putida: implications for the evolution of aromatic
RT   catabolism.";
RL   Mol. Microbiol. 5:2459-2474(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PAO;
RX   PubMed=1339433; DOI=10.1016/s0021-9258(18)42252-1;
RA   Steele M.I., Lorenz D., Hatter K., Park A., Sokatch J.R.;
RT   "Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO
RT   encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate
RT   dehydrogenase.";
RL   J. Biol. Chem. 267:13585-13592(1992).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-15, AND CATALYTIC ACTIVITY.
RX   PubMed=7868591; DOI=10.1128/jb.177.5.1196-1201.1995;
RA   Inoue J., Shaw J.P., Rekik M., Harayama S.;
RT   "Overlapping substrate specificities of benzaldehyde dehydrogenase (the
RT   xylC gene product) and 2-hydroxymuconic semialdehyde dehydrogenase (the
RT   xylG gene product) encoded by TOL plasmid pWW0 of Pseudomonas putida.";
RL   J. Bacteriol. 177:1196-1201(1995).
CC   -!- FUNCTION: 2-hydroxymuconic acid semialdehyde can be converted to 2-
CC       hydroxypent-2,4-dienoate either directly by the action of 2-
CC       hydroxymuconic semialdehyde hydrolase (HMSH) or by the action of three
CC       sequential enzymes, the first of which is HMSD. Can oxidize not only 2-
CC       hydroxymuconic semialdehyde and its analogs but also benzaldehyde and
CC       its analogs.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + NAD(+) + H2O =
CC         (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H(+);
CC         Xref=Rhea:RHEA:34219, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:28080, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:71198; EC=1.2.1.85;
CC         Evidence={ECO:0000269|PubMed:7868591};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8.3 for the oxidation of 2-hydroxymuconic semialdehyde,
CC         and 9.6 for that of benzaldehyde.;
CC   -!- PATHWAY: Aromatic compound metabolism; benzoate degradation via
CC       hydroxylation.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; M64747; AAA26053.1; -; Genomic_DNA.
DR   PIR; E42902; E42902.
DR   RefSeq; WP_011005908.1; NC_003350.1.
DR   AlphaFoldDB; P23105; -.
DR   SMR; P23105; -.
DR   KEGG; ag:AAA26053; -.
DR   BioCyc; MetaCyc:MONOMER-3402; -.
DR   UniPathway; UPA00156; -.
DR   GO; GO:0004029; F:aldehyde dehydrogenase (NAD+) activity; IEA:RHEA.
DR   GO; GO:0043640; P:benzoate catabolic process via hydroxylation; IEA:UniProtKB-UniPathway.
DR   CDD; cd07093; ALDH_F8_HMSADH; 1.
DR   FunFam; 3.40.309.10:FF:000009; Aldehyde dehydrogenase A; 1.
DR   FunFam; 3.40.605.10:FF:000007; NAD/NADP-dependent betaine aldehyde dehydrogenase; 1.
DR   Gene3D; 3.40.605.10; Aldehyde Dehydrogenase, Chain A, domain 1; 1.
DR   Gene3D; 3.40.309.10; Aldehyde Dehydrogenase, Chain A, domain 2; 1.
DR   InterPro; IPR016161; Ald_DH/histidinol_DH.
DR   InterPro; IPR016163; Ald_DH_C.
DR   InterPro; IPR016160; Ald_DH_CS_CYS.
DR   InterPro; IPR029510; Ald_DH_CS_GLU.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH_dom.
DR   InterPro; IPR017628; OHmuconic_semiald_DH.
DR   NCBIfam; TIGR03216; OH_muco_semi_DH; 1.
DR   PANTHER; PTHR43720; 2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE; 1.
DR   PANTHER; PTHR43720:SF2; 2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE; 1.
DR   Pfam; PF00171; Aldedh; 1.
DR   SUPFAM; SSF53720; ALDH-like; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Direct protein sequencing; NAD;
KW   Oxidoreductase; Plasmid.
FT   CHAIN           1..486
FT                   /note="2-hydroxymuconic semialdehyde dehydrogenase"
FT                   /id="PRO_0000056594"
FT   ACT_SITE        254
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        288
FT                   /evidence="ECO:0000250"
FT   CONFLICT        11..12
FT                   /note="EL -> AF (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   486 AA;  51761 MW;  854303F121B2FFBD CRC64;
     MKEIKHFISG ELVGSASGKL FDNVSPANGQ VIGRVHEAGR AEVDAAVRAA RAALKGPWGK
     MTVAERAEIL HRVADGITAR FGEFLEARMP GHRQAEVAGQ PHRHSARRAN FKVFADLLKN
     VANEAFEMAT PDGAGALNYG VRRPKGVIGV ISPWNLPLLL MTWKVGPALA CGNCVVVKPS
     EETPLTATLL GEVMQAAGVP AGVYNVVHGF GGDSAGAFLT EHPDVDAYTF TGETGTGETI
     MRAAAKGVRQ VSLELGGKNA GIVFADCDMD KAIEGTLRSA FANCGQVCLG TERVYVERPI
     FDAFVARLKA GAEALKIGEP NDPEANFGPL ISHKPREKVP SYYQQAVDDG ATVVTGGGVP
     EMPAHLAGGA WVQPTIWTGL ADDSAVVTEE IFGPCCHIRP FDSEEEAIEL ANSLPYGLAS
     AIWTENVRRA HRVAGQIEAG IVWVNSWFLR DLRTAFGGSK QSGIGREGGV HSLEFYTELK
     NICVKL
//