ID GAL7_SALTY Reviewed; 348 AA. AC P22714; DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2002, sequence version 2. DT 04-NOV-2026, entry version 158. DE RecName: Full=Galactose-1-phosphate uridylyltransferase; DE Short=Gal-1-P uridylyltransferase; DE EC=2.7.7.12 {ECO:0000250|UniProtKB:P09148}; DE AltName: Full=UDP-glucose--hexose-1-phosphate uridylyltransferase; GN Name=galT; OrderedLocusNames=STM0775; OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720). OC Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; OC Enterobacterales; Enterobacteriaceae; Salmonella. OX NCBI_TaxID=99287; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=2198256; DOI=10.1128/jb.172.8.4392-4398.1990; RA Houng H.S.H., Kopecko D.J., Baron L.S.; RT "Molecular cloning and physical and functional characterization of the RT Salmonella typhimurium and Salmonella typhi galactose utilization RT operons."; RL J. Bacteriol. 172:4392-4398(1990). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=LT2 / SGSC1412 / ATCC 700720; RX PubMed=11677609; DOI=10.1038/35101614; RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E., RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., RA Wilson R.K.; RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."; RL Nature 413:852-856(2001). CC -!- CATALYTIC ACTIVITY: CC Reaction=alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D- CC glucose 1-phosphate + UDP-alpha-D-galactose; Xref=Rhea:RHEA:13989, CC ChEBI:CHEBI:58336, ChEBI:CHEBI:58601, ChEBI:CHEBI:58885, CC ChEBI:CHEBI:66914; EC=2.7.7.12; CC Evidence={ECO:0000250|UniProtKB:P09148}; CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Evidence={ECO:0000250|UniProtKB:P09148}; CC Note=Binds 1 zinc ion per subunit. Zinc binding seems to play a CC structural role. {ECO:0000250|UniProtKB:P09148}; CC -!- PATHWAY: Carbohydrate metabolism; galactose metabolism. CC -!- SIMILARITY: Belongs to the galactose-1-phosphate uridylyltransferase CC type 1 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M33681; AAA27112.1; -; Genomic_DNA. DR EMBL; AE006468; AAL19713.1; -; Genomic_DNA. DR PIR; B37760; B37760. DR RefSeq; NP_459754.1; NC_003197.2. DR RefSeq; WP_000187473.1; NC_003197.2. DR AlphaFoldDB; P22714; -. DR SMR; P22714; -. DR STRING; 99287.STM0775; -. DR PaxDb; 99287-STM0775; -. DR GeneID; 1252295; -. DR KEGG; stm:STM0775; -. DR PATRIC; fig|99287.12.peg.808; -. DR HOGENOM; CLU_029960_0_0_6; -. DR OMA; AAECNEF; -. DR PhylomeDB; P22714; -. DR BioCyc; SENT99287:STM0775-MONOMER; -. DR UniPathway; UPA00214; -. DR Proteomes; UP000001014; Chromosome. DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. DR GO; GO:0008108; F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity; ISS:UniProtKB. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0033499; P:beta-D-galactose catabolic process via UDP-galactose, Leloir pathway; IBA:GO_Central. DR CDD; cd00608; GalT; 1. DR FunFam; 3.30.428.10:FF:000001; Galactose-1-phosphate uridylyltransferase; 1. DR FunFam; 3.30.428.10:FF:000002; Galactose-1-phosphate uridylyltransferase; 1. DR Gene3D; 3.30.428.10; HIT-like; 2. DR InterPro; IPR001937; GalP_UDPtransf1. DR InterPro; IPR019779; GalP_UDPtransf1_His-AS. DR InterPro; IPR005850; GalP_Utransf_C. DR InterPro; IPR005849; GalP_Utransf_N. DR InterPro; IPR036265; HIT-like_sf. DR NCBIfam; TIGR00209; galT_1; 1. DR NCBIfam; NF008724; PRK11720.1; 1. DR PANTHER; PTHR11943; GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE; 1. DR PANTHER; PTHR11943:SF1; GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE; 1. DR Pfam; PF02744; GalP_UDP_tr_C; 1. DR Pfam; PF01087; GalP_UDP_transf; 1. DR PIRSF; PIRSF000808; GalT; 1. DR SUPFAM; SSF54197; HIT-like; 2. DR PROSITE; PS00117; GAL_P_UDP_TRANSF_I; 1. PE 3: Inferred from homology; KW Carbohydrate metabolism; Galactose metabolism; Iron; Metal-binding; KW Nucleotidyltransferase; Reference proteome; Transferase; Zinc. FT CHAIN 1..348 FT /note="Galactose-1-phosphate uridylyltransferase" FT /id="PRO_0000169896" FT ACT_SITE 166 FT /note="Tele-UMP-histidine intermediate" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10033" FT BINDING 28..31 FT /ligand="UDP-alpha-D-glucose" FT /ligand_id="ChEBI:CHEBI:58885" FT /ligand_note="ligand shared between dimeric partners" FT /evidence="ECO:0000250|UniProtKB:P09148" FT BINDING 52 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10033" FT BINDING 55 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10033" FT BINDING 61 FT /ligand="UDP-alpha-D-glucose" FT /ligand_id="ChEBI:CHEBI:58885" FT /ligand_note="ligand shared between dimeric partners" FT /note="in other chain" FT /evidence="ECO:0000250|UniProtKB:P09148" FT BINDING 77..78 FT /ligand="UDP-alpha-D-glucose" FT /ligand_id="ChEBI:CHEBI:58885" FT /ligand_note="ligand shared between dimeric partners" FT /note="in other chain" FT /evidence="ECO:0000250|UniProtKB:P09148" FT BINDING 115 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10033" FT BINDING 153 FT /ligand="UDP-alpha-D-glucose" FT /ligand_id="ChEBI:CHEBI:58885" FT /ligand_note="ligand shared between dimeric partners" FT /note="in other chain" FT /evidence="ECO:0000250|UniProtKB:P09148" FT BINDING 159..161 FT /ligand="UDP-alpha-D-glucose" FT /ligand_id="ChEBI:CHEBI:58885" FT /ligand_note="ligand shared between dimeric partners" FT /note="in other chain" FT /evidence="ECO:0000250|UniProtKB:P09148" FT BINDING 164 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10033" FT BINDING 168 FT /ligand="UDP-alpha-D-glucose" FT /ligand_id="ChEBI:CHEBI:58885" FT /ligand_note="ligand shared between dimeric partners" FT /note="in other chain" FT /evidence="ECO:0000250|UniProtKB:P09148" FT BINDING 182 FT /ligand="Fe cation" FT /ligand_id="ChEBI:CHEBI:24875" FT /evidence="ECO:0000250|UniProtKB:P09148" FT BINDING 281 FT /ligand="Fe cation" FT /ligand_id="ChEBI:CHEBI:24875" FT /evidence="ECO:0000250|UniProtKB:P09148" FT BINDING 296 FT /ligand="Fe cation" FT /ligand_id="ChEBI:CHEBI:24875" FT /evidence="ECO:0000250|UniProtKB:P09148" FT BINDING 298 FT /ligand="Fe cation" FT /ligand_id="ChEBI:CHEBI:24875" FT /evidence="ECO:0000250|UniProtKB:P09148" FT BINDING 311..312 FT /ligand="UDP-alpha-D-glucose" FT /ligand_id="ChEBI:CHEBI:58885" FT /ligand_note="ligand shared between dimeric partners" FT /evidence="ECO:0000250|UniProtKB:P09148" FT BINDING 316..317 FT /ligand="UDP-alpha-D-glucose" FT /ligand_id="ChEBI:CHEBI:58885" FT /ligand_note="ligand shared between dimeric partners" FT /evidence="ECO:0000250|UniProtKB:P09148" FT BINDING 323 FT /ligand="UDP-alpha-D-glucose" FT /ligand_id="ChEBI:CHEBI:58885" FT /ligand_note="ligand shared between dimeric partners" FT /note="in other chain" FT /evidence="ECO:0000250|UniProtKB:P09148" FT CONFLICT 31 FT /note="Missing (in Ref. 1; AAA27112)" FT /evidence="ECO:0000305" FT CONFLICT 180 FT /note="E -> AE (in Ref. 1)" FT /evidence="ECO:0000305" SQ SEQUENCE 348 AA; 39675 MW; 2D2CA786668A178E CRC64; MTPFNPIDHP HRRYNPLTGQ WVLVSPHRAK RPWQGAQETP SQQMLPAHDP DCFLCAGNTR VTGDKNPDYK GTYVFTNDFA ALMADTPDAP DSHDPLMRCQ SARGTSRVIC FSPDHSKTLP ELSLPALTEI VRTWQTQTAE LGKTYPWVQV FENKGAAMGC SNPHPHGQVW ANSFLPNEAE REDRLQKAYF AEQRSPMLVD YVQRELADGS RTVVETEHWL AVVPYWAAWP FETLLLPKTH VLRITDLSDE QRDSLALALK KLTSRYDNLF QCSFPYSMGW HGAPFNGEEN AHWQLHAHFY PPLLRSATVR KFMVGYEMLA ETQRDLTAEQ AAERLRAVSD IHFRESGV //