ID GLF_ZYMMO Reviewed; 473 AA. AC P21906; Q5NQL4; DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot. DT 15-FEB-2005, sequence version 2. DT 28-JAN-2026, entry version 142. DE RecName: Full=Glucose facilitated diffusion protein {ECO:0000303|PubMed:2254282}; GN Name=glf {ECO:0000303|PubMed:2254282}; OrderedLocusNames=ZMO0366; OS Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4). OC Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria; OC Sphingomonadales; Zymomonadaceae; Zymomonas. OX NCBI_TaxID=264203; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 31821 / ZM4 / CP4; RX PubMed=2254282; DOI=10.1128/jb.172.12.7227-7240.1990; RA Barnell W.O., Yi K.C., Conway T.; RT "Sequence and genetic organization of a Zymomonas mobilis gene cluster that RT encodes several enzymes of glucose metabolism."; RL J. Bacteriol. 172:7227-7240(1990). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 31821 / ZM4 / CP4; RA Ahn J.Y., Kang H.S.; RL Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 31821 / ZM4 / CP4; RX PubMed=15592456; DOI=10.1038/nbt1045; RA Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., RA Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., RA Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y., Kang H.L., Lee S.Y., Lee K.J., RA Kang H.S.; RT "The genome sequence of the ethanologenic bacterium Zymomonas mobilis RT ZM4."; RL Nat. Biotechnol. 23:63-68(2005). RN [4] RP FUNCTION. RC STRAIN=ATCC 31821 / ZM4 / CP4; RX PubMed=8144485; DOI=10.1128/jb.176.7.2133-2135.1994; RA Snoep J.L., Arfman N., Yomano L.P., Fliege R.K., Conway T., Ingram L.O.; RT "Reconstruction of glucose uptake and phosphorylation in a glucose-negative RT mutant of Escherichia coli by using Zymomonas mobilis genes encoding the RT glucose facilitator protein and glucokinase."; RL J. Bacteriol. 176:2133-2135(1994). RN [5] RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES. RC STRAIN=ATCC 29191 / DSM 3580 / JCM 10190 / CECT 560 / NBRC 13756 / RC NCIMB 11199 / NRRL B-4490 / ZM6; RX PubMed=7768841; DOI=10.1128/jb.177.11.3351-3354.1995; RA Weisser P., Kraemer R., Sahm H., Sprenger G.A.; RT "Functional expression of the glucose transporter of Zymomonas mobilis RT leads to restoration of glucose and fructose uptake in Escherichia coli RT mutants and provides evidence for its facilitator action."; RL J. Bacteriol. 177:3351-3354(1995). CC -!- FUNCTION: Allows uptake of glucose by the cell; allows growth on CC glucose minimal medium by E.coli cells impaired in glucose transport CC (PubMed:8144485). Also transports fructose, but has a strong preference CC for glucose (PubMed:7768841). {ECO:0000269|PubMed:7768841, CC ECO:0000269|PubMed:8144485}. CC -!- BIOPHYSICOCHEMICAL PROPERTIES: CC Kinetic parameters: CC KM=4.1 mM for glucose uptake in E.coli at 10 degrees Celsius CC {ECO:0000269|PubMed:7768841}; CC KM=39 mM for fructose uptake in E.coli at 15 degrees Celsius CC {ECO:0000269|PubMed:7768841}; CC Vmax=42 nmol/min/mg enzyme for glucose uptake in E.coli at 10 degrees CC Celsius {ECO:0000269|PubMed:7768841}; CC Vmax=93 nmol/min/mg enzyme for fructose uptake in E.coli at 15 CC degrees Celsius {ECO:0000269|PubMed:7768841}; CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass CC membrane protein {ECO:0000255}. CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar CC transporter (TC 2.A.1.1) family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M60615; AAA27691.1; -; Genomic_DNA. DR EMBL; AF313764; AAG29864.1; -; Genomic_DNA. DR EMBL; AE008692; AAV88990.1; -; Genomic_DNA. DR PIR; A37855; A37855. DR RefSeq; WP_011240287.1; NC_006526.2. DR AlphaFoldDB; P21906; -. DR SMR; P21906; -. DR STRING; 264203.ZMO0366; -. DR TCDB; 2.A.1.1.4; the major facilitator superfamily (mfs). DR GeneID; 79904430; -. DR KEGG; zmo:ZMO0366; -. DR eggNOG; COG0477; Bacteria. DR HOGENOM; CLU_001265_30_5_5; -. DR Proteomes; UP000001173; Chromosome. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005351; F:carbohydrate:proton symporter activity; IEA:TreeGrafter. DR CDD; cd17359; MFS_XylE_like; 1. DR FunFam; 1.20.1250.20:FF:000122; D-xylose transporter XylE; 1. DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 2. DR InterPro; IPR020846; MFS_dom. DR InterPro; IPR005828; MFS_sugar_transport-like. DR InterPro; IPR050360; MFS_Sugar_Transporters. DR InterPro; IPR036259; MFS_trans_sf. DR InterPro; IPR003663; Sugar/inositol_transpt. DR InterPro; IPR005829; Sugar_transporter_CS. DR InterPro; IPR047984; XylE-like. DR NCBIfam; TIGR00879; SP; 1. DR PANTHER; PTHR48022; PLASTIDIC GLUCOSE TRANSPORTER 4; 1. DR PANTHER; PTHR48022:SF2; PLASTIDIC GLUCOSE TRANSPORTER 4; 1. DR Pfam; PF00083; Sugar_tr; 1. DR PRINTS; PR00171; SUGRTRNSPORT. DR SUPFAM; SSF103473; MFS general substrate transporter; 1. DR PROSITE; PS50850; MFS; 1. DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1. DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1. PE 1: Evidence at protein level; KW Cell inner membrane; Cell membrane; Membrane; Reference proteome; KW Sugar transport; Transmembrane; Transmembrane helix; Transport. FT CHAIN 1..473 FT /note="Glucose facilitated diffusion protein" FT /id="PRO_0000050302" FT TOPO_DOM 1..13 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 14..34 FT /note="Helical; Name=1" FT /evidence="ECO:0000255" FT TOPO_DOM 35..59 FT /note="Periplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 60..80 FT /note="Helical; Name=2" FT /evidence="ECO:0000255" FT TOPO_DOM 81..85 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 86..106 FT /note="Helical; Name=3" FT /evidence="ECO:0000255" FT TOPO_DOM 107..112 FT /note="Periplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 113..133 FT /note="Helical; Name=4" FT /evidence="ECO:0000255" FT TOPO_DOM 134..158 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 159..179 FT /note="Helical; Name=5" FT /evidence="ECO:0000255" FT TOPO_DOM 180..187 FT /note="Periplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 188..208 FT /note="Helical; Name=6" FT /evidence="ECO:0000255" FT TOPO_DOM 209..257 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 258..278 FT /note="Helical; Name=7" FT /evidence="ECO:0000255" FT TOPO_DOM 279..303 FT /note="Periplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 304..324 FT /note="Helical; Name=8" FT /evidence="ECO:0000255" FT TOPO_DOM 325..335 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 336..356 FT /note="Helical; Name=9" FT /evidence="ECO:0000255" FT TOPO_DOM 357..366 FT /note="Periplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 367..387 FT /note="Helical; Name=10" FT /evidence="ECO:0000255" FT TOPO_DOM 388..396 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 397..417 FT /note="Helical; Name=11" FT /evidence="ECO:0000255" FT TOPO_DOM 418..429 FT /note="Periplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 430..450 FT /note="Helical; Name=12" FT /evidence="ECO:0000255" FT TOPO_DOM 451..473 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT CONFLICT 145 FT /note="A -> R (in Ref. 1; AAA27691 and 2; AAG29864)" FT /evidence="ECO:0000305" SQ SEQUENCE 473 AA; 50115 MW; 3886E6A18ACD6926 CRC64; MSSESSQGLV TRLALIAAIG GLLFGYDSAV IAAIGTPVDI HFIAPRHLSA TAAASLSGMV VVAVLVGCVT GSLLSGWIGI RFGRRGGLLM SSICFVAAGF GAALTEKLFG TGGSALQIFC FFRFLAGLGI GVVSTLTPTY IAEIAPPDKR GQMVSGQQMA IVTGALTGYI FTWLLAHFGS IDWVNASGWC WSPASEGLIG IAFLLLLLTA PDTPHWLVMK GRHSEASKIL ARLEPQADPN LTIQKIKAGF DKAMDKSSAG LFAFGITVVF AGVSVAAFQQ LVGINAVLYY APQMFQNLGF GADTALLQTI SIGVVNFIFT MIASRVVDRF GRKPLLIWGA LGMAAMMAVL GCCFWFKVGG VLPLASVLLY IAVFGMSWGP VCWVVLSEMF PSSIKGAAMP IAVTGQWLAN ILVNFLFKVA DGSPALNQTF NHGFSYLVFA ALSILGGLIV ARFVPETKGR SLDEIEEMWR SQK //