ID BP08_BPT4 Reviewed; 334 AA. AC P19062; DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1990, sequence version 1. DT 02-SEP-2026, entry version 113. DE RecName: Full=Baseplate wedge protein gp8 {ECO:0000305}; DE AltName: Full=Gene product 8; DE Short=gp8; GN Name=8; OS Enterobacteria phage T4 (Bacteriophage T4). OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; OC Pantevenvirales; Straboviridae; Tevenvirinae; Tequatrovirus. OX NCBI_TaxID=10665; OH NCBI_TaxID=562; Escherichia coli. RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=D; RX PubMed=2402473; DOI=10.1093/nar/18.17.5313; RA Efimov V.P., Prilipov A.G., Mesyanzhinov V.V.; RT "Nucleotide sequences of bacteriophage T4 genes 6, 7 and 8."; RL Nucleic Acids Res. 18:5313-5313(1990). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003; RA Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.; RT "Bacteriophage T4 genome."; RL Microbiol. Mol. Biol. Rev. 67:86-156(2003). RN [3] RP SUBCELLULAR LOCATION. RX PubMed=2403438; DOI=10.1128/jvi.64.1.143-154.1990; RA Watts N.R., Coombs D.H.; RT "Structure of the bacteriophage T4 baseplate as determined by chemical RT cross-linking."; RL J. Virol. 64:143-154(1990). RN [4] RP REVIEW. RX PubMed=14625682; DOI=10.1007/s00018-003-3072-1; RA Leiman P.G., Kanamaru S., Mesyanzhinov V.V., Arisaka F., Rossmann M.G.; RT "Structure and morphogenesis of bacteriophage T4."; RL Cell. Mol. Life Sci. 60:2356-2370(2003). RN [5] RP REVIEW ON FUNCTION. RX PubMed=21129200; DOI=10.1186/1743-422x-7-355; RA Leiman P.G., Arisaka F., van Raaij M.J., Kostyuchenko V.A., Aksyuk A.A., RA Kanamaru S., Rossmann M.G.; RT "Morphogenesis of the T4 tail and tail fibers."; RL Virol. J. 7:355-355(2010). RN [6] RP SUBUNIT. RX PubMed=19896486; DOI=10.1016/j.jmb.2009.10.071; RA Yap M.L., Mio K., Leiman P.G., Kanamaru S., Arisaka F.; RT "The baseplate wedges of bacteriophage T4 spontaneously assemble into RT hubless baseplate-like structure in vitro."; RL J. Mol. Biol. 395:349-360(2010). RN [7] RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 7-334. RX PubMed=12729757; DOI=10.1016/s0022-2836(03)00366-8; RA Leiman P.G., Shneider M.M., Kostyuchenko V.A., Chipman P.R., RA Mesyanzhinov V.V., Rossmann M.G.; RT "Structure and location of gene product 8 in the bacteriophage T4 RT baseplate."; RL J. Mol. Biol. 328:821-833(2003). RN [8] RP STRUCTURE BY ELECTRON MICROSCOPY (17.0 ANGSTROMS) OF THE CONTRACTED TAIL, RP SUBCELLULAR LOCATION, AND FUNCTION. RX PubMed=15315755; DOI=10.1016/j.cell.2004.07.022; RA Leiman P.G., Chipman P.R., Kostyuchenko V.A., Mesyanzhinov V.V., RA Rossmann M.G.; RT "Three-dimensional rearrangement of proteins in the tail of bacteriophage RT T4 on infection of its host."; RL Cell 118:419-429(2004). RN [9] RP STRUCTURE BY ELECTRON MICROSCOPY (4.11 ANGSTROMS), SUBUNIT, SUBCELLULAR RP LOCATION, AND FUNCTION. RX PubMed=27193680; DOI=10.1038/nature17971; RA Taylor N.M., Prokhorov N.S., Guerrero-Ferreira R.C., Shneider M.M., RA Browning C., Goldie K.N., Stahlberg H., Leiman P.G.; RT "Structure of the T4 baseplate and its function in triggering sheath RT contraction."; RL Nature 533:346-352(2016). CC -!- FUNCTION: Intermediate baseplate protein. Involved in the tail CC assembly. {ECO:0000269|PubMed:15315755, ECO:0000269|PubMed:27193680, CC ECO:0000303|PubMed:21129200}. CC -!- SUBUNIT: Homodimer (PubMed:19896486, PubMed:2403438, PubMed:27193680). CC Interacts with gp7. Part of the baseplate macromolecular complex which CC consists of gp5, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 CC (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, CC gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube). CC {ECO:0000269|PubMed:19896486, ECO:0000269|PubMed:27193680}. CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:2403438, CC ECO:0000269|PubMed:27193680}. Note=Present in 12 copies in the CC baseplate. {ECO:0000303|PubMed:21129200}. CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle. CC {ECO:0000305}. CC -!- SIMILARITY: Belongs to the tevenvirinae baseplate structural protein CC gp8 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X15907; CAA34023.1; -; Genomic_DNA. DR EMBL; AF158101; AAD42519.1; -; Genomic_DNA. DR PIR; JQ0658; G8BPT4. DR RefSeq; NP_049766.1; NC_000866.4. DR PDB; 1N7Z; X-ray; 2.00 A; A/B/C/D=1-334. DR PDB; 1N80; X-ray; 2.45 A; A/B/C/D=1-334. DR PDB; 1N8B; X-ray; 2.90 A; A/B/C/D=1-334. DR PDB; 1PDM; EM; 12.00 A; A/B/C/D/E/F/G/H/I/J/K/L=1-334. DR PDB; 1TJA; EM; 16.00 A; A/B=1-334. DR PDB; 5HX2; EM; 3.80 A; B/C=1-334. DR PDB; 5IV5; EM; 4.11 A; CA/CB/D/E/ED/EE/GG/GH/a/b/x/y=1-334. DR PDB; 5IV7; EM; 6.77 A; AA/CB/CC/D/E/ED/EE/T/U/j/k/z=1-334. DR EMDB; EMD-8064; -. DR MDRepo; P19062; -. DR SMR; P19062; -. DR TCDB; 1.K.1.1.1; the gp27/5 t4-baseplate (t4-bp) family. DR GeneID; 1258542; -. DR KEGG; vg:1258542; -. DR OrthoDB; 4009at10239; -. DR EvolutionaryTrace; P19062; -. DR Proteomes; UP000009087; Segment. DR GO; GO:0098025; C:virus tail, baseplate; IDA:UniProtKB. DR GO; GO:0098003; P:viral tail assembly; IEA:UniProtKB-KW. DR FunFam; 2.170.290.10:FF:000001; Baseplate wedge protein gp8; 1. DR Gene3D; 2.60.340.10; baseplate structural protein gp8, domain 1; 1. DR Gene3D; 2.170.290.10; baseplate structural protein gp8, domain 2; 1. DR InterPro; IPR036327; Gp8_sf. DR InterPro; IPR015298; Phage_T4_Gp8. DR Pfam; PF09215; Phage-Gp8; 1. DR SUPFAM; SSF89433; Baseplate structural protein gp8; 1. DR PDBsum; 1N7Z; -. DR PDBsum; 1N80; -. DR PDBsum; 1N8B; -. DR PDBsum; 1PDM; -. DR PDBsum; 1TJA; -. DR PDBsum; 5HX2; -. DR PDBsum; 5IV5; -. DR PDBsum; 5IV7; -. PE 1: Evidence at protein level; KW 3D-structure; Disulfide bond; Reference proteome; Viral baseplate protein; KW Viral release from host cell; Viral tail assembly; Viral tail protein; KW Virion. FT CHAIN 1..334 FT /note="Baseplate wedge protein gp8" FT /id="PRO_0000164998" FT DISULFID 142..153 FT STRAND 9..12 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 14..27 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 28..31 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 37..42 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 49..52 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 58..61 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 65..74 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 75..81 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 83..85 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 86..91 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 111..117 FT /evidence="ECO:0007829|PDB:1N7Z" FT TURN 118..120 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 128..135 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 138..144 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 150..155 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 160..164 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 175..177 FT /evidence="ECO:0007829|PDB:1N8B" FT STRAND 183..185 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 187..194 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 196..202 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 205..210 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 213..218 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 220..223 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 231..234 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 238..241 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 246..249 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 251..254 FT /evidence="ECO:0007829|PDB:1N7Z" FT TURN 255..257 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 259..262 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 269..277 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 294..297 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 299..301 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 307..318 FT /evidence="ECO:0007829|PDB:1N7Z" FT HELIX 319..321 FT /evidence="ECO:0007829|PDB:1N80" FT STRAND 325..328 FT /evidence="ECO:0007829|PDB:1N7Z" FT STRAND 331..333 FT /evidence="ECO:0007829|PDB:1N7Z" SQ SEQUENCE 334 AA; 38008 MW; 4997860773E14899 CRC64; MNDSSVIYRA IVTSKFRTEK MLNFYNSIGS GPDKNTIFIT FGRSEPWSSN ENEVGFAPPY PTDSVLGVTD MWTHMMGTVK VLPSMLDAVI PRRDWGDTRY PDPYTFRIND IVVCNSAPYN ATESGAGWLV YRCLDVPDTG MCSIASLTDK DECLKLGGKW TPSARSMTPP EGRGDAEGTI EPGDGYVWEY LFEIPPDVSI NRCTNEYIVV PWPEELKEDP TRWGYEDNLT WQQDDFGLIY RVKANTIRFK AYLDSVYFPE AALPGNKGFR QISIITNPLE AKAHPNDPNV KAEKDYYDPE DLMRHSGEMI YMENRPPIIM AMDQTEEINI LFTF //