ID   CDX1_MOUSE              Reviewed;         268 AA.
AC   P18111; Q8VCF7;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   02-SEP-2026, entry version 183.
DE   RecName: Full=Homeobox protein CDX-1;
DE   AltName: Full=Caudal-type homeobox protein 1;
GN   Name=Cdx1; Synonyms=Cdx-1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=BALB/cJ;
RX   PubMed=7903305; DOI=10.1016/s0021-9258(19)74240-9;
RA   Hu Y., Kazenwadel J., James R.;
RT   "Isolation and characterization of the murine homeobox gene Cdx-1.
RT   Regulation of expression in intestinal epithelial cells.";
RL   J. Biol. Chem. 268:27214-27225(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Embryonic intestine;
RX   PubMed=2905686; DOI=10.1101/gad.2.12a.1647;
RA   Duprey P., Chowdhury K., Dressler G.R., Balling R., Simon D., Guenet J.-L.,
RA   Gruss P.;
RT   "A mouse gene homologous to the Drosophila gene caudal is expressed in
RT   epithelial cells from the embryonic intestine.";
RL   Genes Dev. 2:1647-1654(1988).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Colon;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Plays a role in transcriptional regulation. Involved in
CC       activated KRAS-mediated transcriptional activation of PRKD1 in
CC       colorectal cancer (CRC) cells. Binds to the PRKD1 promoter in
CC       colorectal cancer (CRC) cells. Could play a role in the terminal
CC       differentiation of the intestine. Binds preferentially to methylated
CC       DNA. {ECO:0000250|UniProtKB:P47902}.
CC   -!- INTERACTION:
CC       P18111; P20226: TBP; Xeno; NbExp=5; IntAct=EBI-21005290, EBI-355371;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Intestinal epithelium.
CC   -!- SIMILARITY: Belongs to the Caudal homeobox family. {ECO:0000305}.
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DR   EMBL; M37163; AAA37412.1; ALT_SEQ; mRNA.
DR   EMBL; M80463; AAA16447.1; ALT_SEQ; Unassigned_DNA.
DR   EMBL; BC019986; AAH19986.1; -; mRNA.
DR   CCDS; CCDS29279.1; -.
DR   PIR; A49303; A49303.
DR   RefSeq; NP_034010.3; NM_009880.4.
DR   AlphaFoldDB; P18111; -.
DR   SMR; P18111; -.
DR   BioGRID; 198663; 6.
DR   FunCoup; P18111; 715.
DR   IntAct; P18111; 10.
DR   STRING; 10090.ENSMUSP00000025521; -.
DR   GlyGen; P18111; 3 sites.
DR   PhosphoSitePlus; P18111; -.
DR   PaxDb; 10090-ENSMUSP00000025521; -.
DR   PeptideAtlas; P18111; -.
DR   ProteomicsDB; 281150; -.
DR   Antibodypedia; 27929; 513 antibodies from 36 providers.
DR   DNASU; 12590; -.
DR   Ensembl; ENSMUST00000025521.9; ENSMUSP00000025521.9; ENSMUSG00000024619.9.
DR   GeneID; 12590; -.
DR   KEGG; mmu:12590; -.
DR   UCSC; uc008fbj.2; mouse.
DR   AGR; MGI:88360; -.
DR   CTD; 1044; -.
DR   MGI; MGI:88360; Cdx1.
DR   VEuPathDB; HostDB:ENSMUSG00000024619; -.
DR   eggNOG; KOG0848; Eukaryota.
DR   GeneTree; ENSGT00940000162069; -.
DR   HOGENOM; CLU_073177_1_0_1; -.
DR   InParanoid; P18111; -.
DR   OMA; DLHHGQP; -.
DR   OrthoDB; 6159439at2759; -.
DR   PhylomeDB; P18111; -.
DR   BioGRID-ORCS; 12590; 0 hits in 80 CRISPR screens.
DR   PRO; PR:P18111; -.
DR   Proteomes; UP000000589; Chromosome 18.
DR   RNAct; P18111; protein.
DR   Bgee; ENSMUSG00000024619; Expressed in left colon and 42 other cell types or tissues.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR   GO; GO:0008327; F:methyl-CpG binding; ISS:UniProtKB.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:GO_Central.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR   GO; GO:0009948; P:anterior/posterior axis specification; IBA:GO_Central.
DR   GO; GO:0009952; P:anterior/posterior pattern specification; IMP:MGI.
DR   GO; GO:0060349; P:bone morphogenesis; IMP:MGI.
DR   GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR   GO; GO:0048565; P:digestive tract development; IBA:GO_Central.
DR   GO; GO:0009880; P:embryonic pattern specification; IBA:GO_Central.
DR   GO; GO:0007389; P:pattern specification process; IMP:MGI.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR   GO; GO:0014807; P:regulation of somitogenesis; IMP:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   FunFam; 1.10.10.60:FF:000089; Caudal type homeobox 4; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR006820; Caudal_activation_dom.
DR   InterPro; IPR047152; Caudal_homeobox.
DR   InterPro; IPR001356; HD.
DR   InterPro; IPR020479; HD_metazoa.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR009057; Homeodomain-like_sf.
DR   InterPro; IPR000047; HTH_motif.
DR   PANTHER; PTHR24332; HOMEOBOX PROTEIN CDX; 1.
DR   PANTHER; PTHR24332:SF16; HOMEOBOX PROTEIN CDX-1; 1.
DR   Pfam; PF04731; Caudal_act; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00024; HOMEOBOX.
DR   PRINTS; PR00031; HTHREPRESSR.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   1: Evidence at protein level;
KW   Activator; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..268
FT                   /note="Homeobox protein CDX-1"
FT                   /id="PRO_0000048847"
FT   DNA_BIND        154..213
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..152
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          157..178
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250|UniProtKB:P47902"
FT   REGION          196..207
FT                   /note="Interaction with 5-mCpG DNA"
FT                   /evidence="ECO:0000250|UniProtKB:P47902"
FT   REGION          209..268
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        29..42
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        54..67
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        73..91
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        92..108
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        115..128
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        229..246
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        15
FT                   /note="P -> Q (in Ref. 3; AAH19986)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        146
FT                   /note="G -> C (in Ref. 2; AAA37412)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        157
FT                   /note="Y -> S (in Ref. 2; AAA37412)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   268 AA;  28436 MW;  85F45B2A3E12B2AE CRC64;
     MYVGYVLDKD SPVYPGPARP SSLGLGPPTY APPGPAPAPP QYPDFAGYTH VEPAPAPPPT
     WAAPFPAPKD DWAAAYGPGP TASAASPAPL AFGPPPDFSP VPAPPGPGPG ILAQSLGAPG
     APSSPGAPRR TPYEWMRRSV AAAGGGGSGK TRTKDKYRVV YTDHQRLELE KEFHYSRYIT
     IRRKSELAAN LGLTERQVKI WFQNRRAKER KVNKKKQQQQ QPLPPTQLPL PLDGTPTPSG
     PPLGSLCPTN AGLLGTPSPV PVKEEFLP
//