ID   MASY_GOSHI              Reviewed;         567 AA.
AC   P17432;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   04-NOV-2026, entry version 106.
DE   RecName: Full=Malate synthase, glyoxysomal;
DE            EC=2.3.3.9;
OS   Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX   NCBI_TaxID=3635;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Seed;
RX   PubMed=2362818; DOI=10.1093/nar/18.12.3643;
RA   Turley R.B., Choe S.M., Ni W., Trelease R.N.;
RT   "Nucleotide sequence of cottonseed malate synthase.";
RL   Nucleic Acids Res. 18:3643-3643(1990).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC         Evidence={ECO:0000250|UniProtKB:Q9LZC3};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:A7RM90};
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 2/2.
CC   -!- SUBCELLULAR LOCATION: Glyoxysome.
CC   -!- SIMILARITY: Belongs to the malate synthase family. {ECO:0000305}.
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DR   EMBL; X52305; CAA36546.1; -; mRNA.
DR   PIR; S12735; SYCNMU.
DR   AlphaFoldDB; P17432; -.
DR   SMR; P17432; -.
DR   STRING; 3635.P17432; -.
DR   PaxDb; 3635-P17432; -.
DR   OMA; AAFIPRK; -.
DR   UniPathway; UPA00703; UER00720.
DR   Proteomes; UP000818029; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0009514; C:glyoxysome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004474; F:malate synthase activity; IBA:GO_Central.
DR   GO; GO:0006097; P:glyoxylate cycle; IBA:GO_Central.
DR   CDD; cd00727; malate_synt_A; 1.
DR   FunFam; 1.20.1220.12:FF:000001; Malate synthase; 1.
DR   FunFam; 3.20.20.360:FF:000001; Malate synthase; 1.
DR   Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR   Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR   InterPro; IPR044856; Malate_synth_C_sf.
DR   InterPro; IPR011076; Malate_synth_sf.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   InterPro; IPR001465; Malate_synthase_TIM.
DR   InterPro; IPR048355; MS_C.
DR   InterPro; IPR048356; MS_N.
DR   InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR   NCBIfam; TIGR01344; malate_syn_A; 1.
DR   PANTHER; PTHR42902; MALATE SYNTHASE; 1.
DR   PANTHER; PTHR42902:SF1; MALATE SYNTHASE 1-RELATED; 1.
DR   Pfam; PF20659; MS_C; 1.
DR   Pfam; PF20656; MS_N; 1.
DR   Pfam; PF01274; MS_TIM-barrel; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; Malate synthase G; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   2: Evidence at transcript level;
KW   Glyoxylate bypass; Glyoxysome; Magnesium; Metal-binding; Peroxisome;
KW   Reference proteome; Transferase.
FT   CHAIN           1..567
FT                   /note="Malate synthase, glyoxysomal"
FT                   /id="PRO_0000166869"
FT   MOTIF           565..567
FT                   /note="Microbody targeting signal"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        182
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P9WK16"
FT   ACT_SITE        468
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P9WK16"
FT   BINDING         135
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:D4GTL2"
FT   BINDING         269
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:D4GTL2"
FT   BINDING         311
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:D4GTL2"
SQ   SEQUENCE   567 AA;  64669 MW;  AC69D3BE20C91204 CRC64;
     MIGLGSYGYT APSSKKINAY DVPQGVDIRG RFDEEFAKIL TKDALQFVAD LQREFRNHIK
     YAMECRKEAK RRYNEGALPG FDPATRYIRE GKWTCVPFPP AVADRRVEIT GPVERKMIIN
     ALNSGAKVFM ADFEDALSPS WENLMRGQIN LKDAVEGTIT FNDKARNRVY KLNNEIAKLF
     VRPRGWHLPE AHIFIDGEPA TGCLVDFGLY FYHNYATFRN TQGQGFGPFF YLPKMENSRE
     AKIWNSVFEK AEKMAGIEKG SIRATVLIET LPAVFQMDEI FYELRDHSVG LNCGRWDYIF
     SYVKTFQGHP DRLLPDRGQV GMTQHFMRSY SDLLIRTCHR RGVHAMGGMA AQIPIRDDPT
     ANEAAFELVR KDKQREVKAG HDGTWAAHPG LIKTCMEVFT NNMGNTPNQI ETVKRDYASN
     LTEDDLLQRP RGVRTMEGLR LNTRVGIQYL AAWLTGSGSV PLYNLMEDAA TAEISRVQIW
     QWLKYGVELD GDGLGVRVNH VFGRVVEEEM ARIEREVGKE KFKKGMYKEA CKIFTRQCTA
     STLDDFLTLD AYNYIVIHHP KDVSSKL
//