ID IAAC3_WHEAT Reviewed; 168 AA. AC P17314; DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1990, sequence version 1. DT 02-SEP-2026, entry version 122. DE RecName: Full=Alpha-amylase/trypsin inhibitor CM3; DE AltName: Full=Chloroform/methanol-soluble protein CM3; DE Flags: Precursor; OS Triticum aestivum (Wheat). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum. OX NCBI_TaxID=4565; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Chinese Spring; TISSUE=Endosperm; RX PubMed=2102861; DOI=10.1007/bf00016517; RA Garcia-Maroto F., Marana C., Mena M., Garcia-Olmedo F., Carbonero P.; RT "Cloning of cDNA and chromosomal location of genes encoding the three types RT of subunits of the wheat tetrameric inhibitor of insect alpha-amylase."; RL Plant Mol. Biol. 14:845-853(1990). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Agathe; TISSUE=Seed; RA Gautier M.-F., Alary R., Lullien V., Joudrier P.; RL Submitted (JUN-1991) to the EMBL/GenBank/DDBJ databases. RN [3] RP PROTEIN SEQUENCE OF 26-60. RC STRAIN=cv. Turgidum; TISSUE=Endosperm; RA Shewry P.R., Lafiandra D., Salcedo G., Aragoncillo C., Garcia-Olmedo F., RA Lew E.J.-L., Dietler M.D., Kasarda D.D.; RT "N-terminal amino acid sequence of chloroform/methanol-soluble proteins and RT albumins from endosperm of wheat, barley and related species."; RL FEBS Lett. 175:359-363(1984). CC -!- FUNCTION: Alpha-amylase/trypsin inhibitor. It could be involved in CC insect defense mechanisms. CC -!- SUBUNIT: Subunit of the tetrameric inhibitor. CC -!- SUBCELLULAR LOCATION: Secreted. CC -!- TISSUE SPECIFICITY: Developing endosperm. CC -!- PTM: Five disulfide bonds, which are essential for the inhibitor CC activity, are probably present. {ECO:0000305}. CC -!- MISCELLANEOUS: CM proteins would be involved in the cooking quality of CC pasta. CC -!- SIMILARITY: Belongs to the protease inhibitor I6 (cereal trypsin/alpha- CC amylase inhibitor) family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X17574; CAA35597.1; -; mRNA. DR EMBL; X61032; CAA43367.1; -; mRNA. DR PIR; S10029; S10029. DR RefSeq; NP_001392679.1; NM_001405750.1. DR AlphaFoldDB; P17314; -. DR SMR; P17314; -. DR STRING; 4565.P17314; -. DR Allergome; 1051; Tri a 30. DR Allergome; 8191; Tri a 30.0101. DR MEROPS; I06.004; -. DR PaxDb; 4565-P17314; -. DR EnsemblPlants; TraesARI4B03G02424440.1; TraesARI4B03G02424440.1.CDS1; TraesARI4B03G02424440. DR EnsemblPlants; TraesCAD_scaffold_059374_01G000200.1; TraesCAD_scaffold_059374_01G000200.1; TraesCAD_scaffold_059374_01G000200. DR EnsemblPlants; TraesCLE_scaffold_030329_01G000100.1; TraesCLE_scaffold_030329_01G000100.1; TraesCLE_scaffold_030329_01G000100. DR EnsemblPlants; TraesCS4B02G328100.1; TraesCS4B02G328100.1.cds1; TraesCS4B02G328100. DR EnsemblPlants; TraesCS4B03G0849100.1; TraesCS4B03G0849100.1.CDS1; TraesCS4B03G0849100. DR EnsemblPlants; TraesJAG4B03G02385240.1; TraesJAG4B03G02385240.1.CDS1; TraesJAG4B03G02385240. DR EnsemblPlants; TraesJUL4B03G02406420.1; TraesJUL4B03G02406420.1.CDS1; TraesJUL4B03G02406420. DR EnsemblPlants; TraesKAR4B01G0414260.1; cds.TraesKAR4B01G0414260.1; TraesKAR4B01G0414260. DR EnsemblPlants; TraesLAC4B03G02340480.1; TraesLAC4B03G02340480.1.CDS1; TraesLAC4B03G02340480. DR EnsemblPlants; TraesLDM4B03G02388410.1; TraesLDM4B03G02388410.1.CDS1; TraesLDM4B03G02388410. DR EnsemblPlants; TraesMAC4B03G02386240.1; TraesMAC4B03G02386240.1.CDS1; TraesMAC4B03G02386240. DR EnsemblPlants; TraesNOR4B03G02405190.1; TraesNOR4B03G02405190.1.CDS1; TraesNOR4B03G02405190. DR EnsemblPlants; TraesPARA_EIv1.0_1391150.1; TraesPARA_EIv1.0_1391150.1.CDS1; TraesPARA_EIv1.0_1391150. DR EnsemblPlants; TraesRN4B0100879200.1; TraesRN4B0100879200.1; TraesRN4B0100879200. DR EnsemblPlants; TraesROB_scaffold_059406_01G000100.1; TraesROB_scaffold_059406_01G000100.1; TraesROB_scaffold_059406_01G000100. DR EnsemblPlants; TraesSTA4B03G02381920.1; TraesSTA4B03G02381920.1.CDS1; TraesSTA4B03G02381920. DR EnsemblPlants; TraesSYM4B03G02414270.1; TraesSYM4B03G02414270.1.CDS1; TraesSYM4B03G02414270. DR EnsemblPlants; TraesWEE_scaffold_013736_01G000500.1; TraesWEE_scaffold_013736_01G000500.1; TraesWEE_scaffold_013736_01G000500. DR GeneID; 543281; -. DR Gramene; TraesARI4B03G02424440.1; TraesARI4B03G02424440.1.CDS1; TraesARI4B03G02424440. DR Gramene; TraesCAD_scaffold_059374_01G000200.1; TraesCAD_scaffold_059374_01G000200.1; TraesCAD_scaffold_059374_01G000200. DR Gramene; TraesCLE_scaffold_030329_01G000100.1; TraesCLE_scaffold_030329_01G000100.1; TraesCLE_scaffold_030329_01G000100. DR Gramene; TraesCS4B02G328100.1; TraesCS4B02G328100.1.cds1; TraesCS4B02G328100. DR Gramene; TraesCS4B03G0849100.1; TraesCS4B03G0849100.1.CDS1; TraesCS4B03G0849100. DR Gramene; TraesJAG4B03G02385240.1; TraesJAG4B03G02385240.1.CDS1; TraesJAG4B03G02385240. DR Gramene; TraesJUL4B03G02406420.1; TraesJUL4B03G02406420.1.CDS1; TraesJUL4B03G02406420. DR Gramene; TraesKAR4B01G0414260.1; cds.TraesKAR4B01G0414260.1; TraesKAR4B01G0414260. DR Gramene; TraesLAC4B03G02340480.1; TraesLAC4B03G02340480.1.CDS1; TraesLAC4B03G02340480. DR Gramene; TraesLDM4B03G02388410.1; TraesLDM4B03G02388410.1.CDS1; TraesLDM4B03G02388410. DR Gramene; TraesMAC4B03G02386240.1; TraesMAC4B03G02386240.1.CDS1; TraesMAC4B03G02386240. DR Gramene; TraesNOR4B03G02405190.1; TraesNOR4B03G02405190.1.CDS1; TraesNOR4B03G02405190. DR Gramene; TraesPARA_EIv1.0_1391150.1; TraesPARA_EIv1.0_1391150.1.CDS1; TraesPARA_EIv1.0_1391150. DR Gramene; TraesRN4B0100879200.1; TraesRN4B0100879200.1; TraesRN4B0100879200. DR Gramene; TraesROB_scaffold_059406_01G000100.1; TraesROB_scaffold_059406_01G000100.1; TraesROB_scaffold_059406_01G000100. DR Gramene; TraesSTA4B03G02381920.1; TraesSTA4B03G02381920.1.CDS1; TraesSTA4B03G02381920. DR Gramene; TraesSYM4B03G02414270.1; TraesSYM4B03G02414270.1.CDS1; TraesSYM4B03G02414270. DR Gramene; TraesWEE_scaffold_013736_01G000500.1; TraesWEE_scaffold_013736_01G000500.1; TraesWEE_scaffold_013736_01G000500. DR HOGENOM; CLU_113497_1_1_1; -. DR OMA; PAYCRCT; -. DR OrthoDB; 603651at2759; -. DR Proteomes; UP000019116; Chromosome 4B. DR ExpressionAtlas; P17314; baseline and differential. DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell. DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:InterPro. DR CDD; cd00261; AAI_SS; 1. DR Gene3D; 1.10.110.10; Plant lipid-transfer and hydrophobic proteins; 1. DR InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib. DR InterPro; IPR006105; Allergen/tryp_amyl_inhib_CS. DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf. DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store. DR PANTHER; PTHR34481:SF5; ALPHA-AMYLASE_TRYPSIN INHIBITOR CM3; 1. DR PANTHER; PTHR34481; TRYPSIN/FACTOR XIIA INHIBITOR-RELATED; 1. DR Pfam; PF00234; Tryp_alpha_amyl; 1. DR PRINTS; PR00808; AMLASEINHBTR. DR SMART; SM00499; AAI; 1. DR SUPFAM; SSF47699; Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin; 1. DR PROSITE; PS00426; CEREAL_TRYP_AMYL_INH; 1. PE 1: Evidence at protein level; KW Alpha-amylase inhibitor; Direct protein sequencing; Disulfide bond; KW Protease inhibitor; Reference proteome; Secreted; KW Serine protease inhibitor; Signal. FT SIGNAL 1..25 FT /evidence="ECO:0000269|Ref.3" FT CHAIN 26..168 FT /note="Alpha-amylase/trypsin inhibitor CM3" FT /id="PRO_0000014346" SQ SEQUENCE 168 AA; 18221 MW; A950FF272E90D23C CRC64; MACKSSCSLL LLAAVLLSVL AAASASGSCV PGVAFRTNLL PHCRDYVLQQ TCGTFTPGSK LPEWMTSASI YSPGKPYLAK LYCCQELAEI SQQCRCEALR YFIALPVPSQ PVDPRSGNVG ESGLIDLPGC PREMQWDFVR LLVAPGQCNL ATIHNVRYCP AVEQPLWI //