ID   MHPB_CUPNE              Reviewed;         313 AA.
AC   P17295;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   18-JUN-2025, entry version 88.
DE   RecName: Full=2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase;
DE            EC=1.13.11.16;
DE   AltName: Full=3-carboxyethylcatechol 2,3-dioxygenase;
GN   Name=mhpB; Synonyms=mcpI;
OS   Cupriavidus necator (Alcaligenes eutrophus) (Ralstonia eutropha).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
OC   Burkholderiales; Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=106590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JMP222;
RX   PubMed=2356133; DOI=10.1093/nar/18.11.3405;
RA   Kabisch M., Fortnagel P.;
RT   "Nucleotide sequence of metapyrocatechase I (catechol 2,3-oxygenase I) gene
RT   mpcI from Alcaligenes eutrophus JMP222.";
RL   Nucleic Acids Res. 18:3405-3405(1990).
RN   [2]
RP   FUNCTION IN CATABOLISM OF 3-HYDROXY DERIVATIVES OF PHENYLPROPIONIC ACID,
RP   COFACTOR, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=8752345; DOI=10.1128/jb.178.17.5249-5256.1996;
RA   Spence E.L., Kawamukai M., Sanvoisin J., Braven H., Bugg T.D.H.;
RT   "Catechol dioxygenases from Escherichia coli (MhpB) and Alcaligenes
RT   eutrophus (MpcI): sequence analysis and biochemical properties of a third
RT   family of extradiol dioxygenases.";
RL   J. Bacteriol. 178:5249-5256(1996).
CC   -!- FUNCTION: Catalyzes the non-heme iron(II)-dependent oxidative cleavage
CC       of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid
CC       into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-
CC       ketononatrienedioate, respectively. Also catalyzes the cleavage of
CC       catechol. {ECO:0000269|PubMed:8752345}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-(2,3-dihydroxyphenyl)propanoate + O2 = (2Z,4E)-2-hydroxy-6-
CC         oxonona-2,4-dienedioate + H(+); Xref=Rhea:RHEA:23840,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:46951,
CC         ChEBI:CHEBI:66887; EC=1.13.11.16;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + O2 = (2Z,4E,7E)-2-
CC         hydroxy-6-oxonona-2,4,7-trienedioate + H(+); Xref=Rhea:RHEA:25054,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:58642,
CC         ChEBI:CHEBI:66888; EC=1.13.11.16;
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000269|PubMed:8752345};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=7.1 uM for 2,3-dihydroxyphenylpropionic acid (at 20 degrees
CC         Celsius and pH 8) {ECO:0000269|PubMed:8752345};
CC         KM=14 uM for 2,3-dihydroxycinnamic acid (at 20 degrees Celsius and pH
CC         8) {ECO:0000269|PubMed:8752345};
CC         KM=59 uM for 3-ethylcatechol (at 20 degrees Celsius and pH 8)
CC         {ECO:0000269|PubMed:8752345};
CC         KM=130 uM for 3-methylcatechol (at 20 degrees Celsius and pH 8)
CC         {ECO:0000269|PubMed:8752345};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropanoate degradation.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the LigB/MhpB extradiol dioxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; X52414; CAA36665.1; -; Genomic_DNA.
DR   PIR; S10154; S10154.
DR   AlphaFoldDB; P17295; -.
DR   SMR; P17295; -.
DR   SwissLipids; SLP:000001890; -.
DR   SABIO-RK; P17295; -.
DR   UniPathway; UPA00714; -.
DR   GO; GO:0047070; F:3-carboxyethylcatechol 2,3-dioxygenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008198; F:ferrous iron binding; IEA:InterPro.
DR   GO; GO:0019380; P:3-phenylpropionate catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd07365; MhpB_like; 1.
DR   Gene3D; 3.40.830.10; LigB-like; 1.
DR   HAMAP; MF_01653; MhpB; 1.
DR   InterPro; IPR023789; DHPP/DHXA_dioxygenase.
DR   InterPro; IPR004183; Xdiol_dOase_suB.
DR   NCBIfam; NF009908; PRK13370.1-2; 1.
DR   NCBIfam; NF009910; PRK13370.1-4; 1.
DR   Pfam; PF02900; LigB; 1.
DR   SUPFAM; SSF53213; LigB-like; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; Iron; Oxidoreductase.
FT   CHAIN           1..313
FT                   /note="2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic
FT                   acid 1,2-dioxygenase"
FT                   /id="PRO_0000085102"
FT   ACT_SITE        115
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        179
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   313 AA;  33143 MW;  E1506B3785E9D0F9 CRC64;
     MPIQLECLSH TPLHGYVDPA PEVVAEVERV QAAARDRVRA FDPELVVVFA PDHFNGFFYD
     VMPPFCIGAA ATAIGDFKSL AGKLPVPADL ALSLAESVMA ADIDVALSHR MQVDHGCADA
     LAALTGSLHR YPVIPVFINS VAPPMATLRR ARLLGDAVGR FLSRAGKRVL VVGSGGISHE
     PPVPELAGAS EEVAERLIAG RNPSPESAAR QARTVAAAKS FVAGDSHLHP LNPEWDRAFL
     SLLASGELTA VDGMTNDAIT RDGGKSAHEI RTWVAAFGAL AAYGPYRASL DFYRAIPEWI
     AGFATMHAEP AAV
//