ID   URED_PROMH              Reviewed;         274 AA.
AC   P17089; B4EXN2;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   09-APR-2025, entry version 112.
DE   RecName: Full=Urease accessory protein UreD {ECO:0000255|HAMAP-Rule:MF_01384};
GN   Name=ureD {ECO:0000255|HAMAP-Rule:MF_01384};
GN   OrderedLocusNames=PMI3682;
OS   Proteus mirabilis (strain HI4320).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Morganellaceae; Proteus.
OX   NCBI_TaxID=529507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2687233; DOI=10.1128/jb.171.12.6414-6422.1989;
RA   Jones B.D., Mobley H.L.T.;
RT   "Proteus mirabilis urease: nucleotide sequence determination and comparison
RT   with jack bean urease.";
RL   J. Bacteriol. 171:6414-6422(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HI4320;
RX   PubMed=18375554; DOI=10.1128/jb.01981-07;
RA   Pearson M.M., Sebaihia M., Churcher C., Quail M.A., Seshasayee A.S.,
RA   Luscombe N.M., Abdellah Z., Arrosmith C., Atkin B., Chillingworth T.,
RA   Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H.,
RA   Rabbinowitsch E., Walker D., Whithead S., Thomson N.R., Rather P.N.,
RA   Parkhill J., Mobley H.L.T.;
RT   "Complete genome sequence of uropathogenic Proteus mirabilis, a master of
RT   both adherence and motility.";
RL   J. Bacteriol. 190:4027-4037(2008).
RN   [3]
RP   UREASE AS A VIRULENCE FACTOR.
RX   PubMed=2180821; DOI=10.1128/iai.58.4.1120-1123.1990;
RA   Jones B.D., Lockatell C.V., Johnson D.E., Warren J.W., Mobley H.L.T.;
RT   "Construction of a urease-negative mutant of Proteus mirabilis: analysis of
RT   virulence in a mouse model of ascending urinary tract infection.";
RL   Infect. Immun. 58:1120-1123(1990).
RN   [4]
RP   INDUCTION.
RX   PubMed=7678244; DOI=10.1128/jb.175.2.465-473.1993;
RA   Nicholson E.B., Concaugh E.A., Foxall P.A., Island M.D., Mobley H.L.T.;
RT   "Proteus mirabilis urease: transcriptional regulation by UreR.";
RL   J. Bacteriol. 175:465-473(1993).
RN   [5]
RP   PROBABLE OPERON STRUCTURE, AND MUTAGENESIS OF ARG-270.
RX   PubMed=7559355; DOI=10.1128/jb.177.19.5653-5660.1995;
RA   Island M.D., Mobley H.L.T.;
RT   "Proteus mirabilis urease: operon fusion and linker insertion analysis of
RT   ure gene organization, regulation, and function.";
RL   J. Bacteriol. 177:5653-5660(1995).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01384}.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01384}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01384}.
CC   -!- INDUCTION: By urea. {ECO:0000269|PubMed:7678244}.
CC   -!- SIMILARITY: Belongs to the UreD family. {ECO:0000255|HAMAP-
CC       Rule:MF_01384}.
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DR   EMBL; M31834; AAA25666.1; -; Genomic_DNA.
DR   EMBL; AM942759; CAR47180.1; -; Genomic_DNA.
DR   PIR; A43719; A43719.
DR   RefSeq; WP_004248933.1; NC_010554.1.
DR   AlphaFoldDB; P17089; -.
DR   SMR; P17089; -.
DR   EnsemblBacteria; CAR47180; CAR47180; PMI3682.
DR   GeneID; 6801576; -.
DR   KEGG; pmr:PMI3682; -.
DR   eggNOG; COG0829; Bacteria.
DR   HOGENOM; CLU_056339_0_0_6; -.
DR   Proteomes; UP000008319; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01384; UreD; 1.
DR   InterPro; IPR002669; UreD.
DR   PANTHER; PTHR33643; UREASE ACCESSORY PROTEIN D; 1.
DR   PANTHER; PTHR33643:SF1; UREASE ACCESSORY PROTEIN D; 1.
DR   Pfam; PF01774; UreD; 1.
PE   1: Evidence at protein level;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..274
FT                   /note="Urease accessory protein UreD"
FT                   /id="PRO_0000067613"
FT   MUTAGEN         270
FT                   /note="R->RRRRI: Reduces activity 14-fold."
FT                   /evidence="ECO:0000269|PubMed:7559355"
SQ   SEQUENCE   274 AA;  31009 MW;  5B52FAAFD3573A9E CRC64;
     MPDFSEKGWL ADIALRYELK RGKTCLTEKR HLGPLMVQRP FYPEQGVAHT YLLHPPGGVV
     GGDTLSININ VQPYAHALLT TPGATKFYRS AGGTASQTQT LTVAQEGFLE WLPQENIFFP
     DAQVCLTTHI HLASSAKFIG WEMQCFGRPV LNEWFETGKV KGRLNFYVDE RLILTESMRV
     EGLQKQAAAM REFPMFGSLY IYPATDALKE IIQHHLEKVN PLVEYGLTDV DGILVLRVLG
     TQTEPMMACF AQVWQIVRQH WLGYCPEPPR IWAT
//