ID   IAA1_HORVU              Reviewed;         146 AA.
AC   P16968; Q43483;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   04-NOV-2026, entry version 120.
DE   RecName: Full=Alpha-amylase inhibitor BMAI-1;
DE   AltName: Full=Alpha-amylase flour inhibitor;
DE   AltName: Allergen=Hor v 1;
DE   Flags: Precursor; Fragment;
GN   Name=IAM1;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Abyssinian 2231; TISSUE=Endosperm;
RX   PubMed=1421148; DOI=10.1007/bf00040604;
RA   Mena M., Sanchez-Monge R., Gomez L., Salcedo G., Carbonero P.;
RT   "A major barley allergen associated with baker's asthma disease is a
RT   glycosylated monomeric inhibitor of insect alpha-amylase: cDNA cloning and
RT   chromosomal location of the gene.";
RL   Plant Mol. Biol. 20:451-458(1992).
RN   [2]
RP   PROTEIN SEQUENCE OF 15-34.
RC   STRAIN=cv. Bomi;
RX   PubMed=2785932; DOI=10.1016/0014-5793(89)80444-2;
RA   Barber D., Sanchez-Monge R., Gomez L., Carpizo J., Armentia A.,
RA   Lopez-Otin C., Juan F., Salcedo G.;
RT   "A barley flour inhibitor of insect alpha-amylase is a major allergen
RT   associated with baker's asthma disease.";
RL   FEBS Lett. 248:119-122(1989).
CC   -!- FUNCTION: Could be involved in insect defense mechanisms. Inhibits
CC       insect-type alpha-amylase.
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Endosperm.
CC   -!- PTM: Five disulfide bonds, which are essential for the inhibitor
CC       activity, are probably present. {ECO:0000305}.
CC   -!- PTM: Glycosylated.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Involved in baker's
CC       asthma.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I6 (cereal trypsin/alpha-
CC       amylase inhibitor) family. {ECO:0000305}.
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DR   EMBL; X63517; CAA45085.1; -; mRNA.
DR   PIR; S26197; S26197.
DR   SMR; P16968; -.
DR   Allergome; 3328; Hor v 15.0101.
DR   Allergome; 418; Hor v 15.
DR   GlyCosmos; P16968; 1 site, No reported glycans.
DR   OMA; AIGDEWC; -.
DR   CDD; cd00261; AAI_SS; 1.
DR   FunFam; 1.10.110.10:FF:000004; Alpha-amylase inhibitor 0.19; 1.
DR   Gene3D; 1.10.110.10; Plant lipid-transfer and hydrophobic proteins; 1.
DR   InterPro; IPR002411; Allergen/amylase_inhib_rice.
DR   InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR   InterPro; IPR006105; Allergen/tryp_amyl_inhib_CS.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR   PANTHER; PTHR34481:SF8; SEED ALLERGENIC PROTEIN RAG1; 1.
DR   PANTHER; PTHR34481; TRYPSIN/FACTOR XIIA INHIBITOR-RELATED; 1.
DR   Pfam; PF00234; Tryp_alpha_amyl; 1.
DR   PIRSF; PIRSF001657; Allergen/amylase_inhib; 1.
DR   PRINTS; PR00808; AMLASEINHBTR.
DR   PRINTS; PR00809; RAGALLERGEN.
DR   SMART; SM00499; AAI; 1.
DR   SUPFAM; SSF47699; Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin; 1.
DR   PROSITE; PS00426; CEREAL_TRYP_AMYL_INH; 1.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015066; F:alpha-amylase inhibitor activity; IDA:CACAO.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:InterPro.
DR   AlphaFoldDB; P16968; -.
PE   1: Evidence at protein level;
KW   Allergen; Alpha-amylase inhibitor; Direct protein sequencing;
KW   Disulfide bond; Glycoprotein; Secreted; Signal.
FT   SIGNAL          <1..14
FT                   /evidence="ECO:0000269|PubMed:2785932"
FT   CHAIN           15..146
FT                   /note="Alpha-amylase inhibitor BMAI-1"
FT                   /id="PRO_0000014355"
FT   CARBOHYD        125
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   NON_TER         1
SQ   SEQUENCE   146 AA;  15816 MW;  73F505186D1A48D8 CRC64;
     PTSVAVDQGS MVSNSPGEWC WPGMGYPVYP FPRCRALVKS QCAGGQVVES IQKDCCRQIA
     AIGDEWCICG ALGSMRGSMY KELGVALADD KATVAEVFPG CRTEVMDRAV ASLPAVCNQY
     IPNTNGTDGV CYWLSYYQPP RQMSSR
//