ID   HXB2_HUMAN              Reviewed;         356 AA.
AC   P14652; P10913; P17485;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   02-SEP-2026, entry version 214.
DE   RecName: Full=Homeobox protein Hox-B2;
DE   AltName: Full=Homeobox protein Hox-2.8;
DE   AltName: Full=Homeobox protein Hox-2H;
DE   AltName: Full=K8;
GN   Name=HOXB2; Synonyms=HOX2H;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2574852; DOI=10.1093/nar/17.24.10385;
RA   Acampora D., D'Esposito M., Faiella A., Pannese M., Migliaccio E.,
RA   Morelli F., Stornaiuolo A., Nigro V., Simeone A., Boncinelli E.;
RT   "The human HOX gene family.";
RL   Nucleic Acids Res. 17:10385-10402(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 132-208.
RC   TISSUE=Placenta;
RX   PubMed=2570724; DOI=10.1111/j.1432-0436.1989.tb00598.x;
RA   Giampaolo A., Acampora D., Zappavigna V., Pannese M., D'Esposito M.,
RA   Care A., Faiella A., Stornaiuolo A., Russo G., Simeone A., Boncinelli E.,
RA   Peschle C.;
RT   "Differential expression of human HOX-2 genes along the anterior-posterior
RT   axis in embryonic central nervous system.";
RL   Differentiation 40:191-197(1989).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 143-208.
RX   PubMed=2576652; DOI=10.1139/g89-133;
RA   Boncinelli E., Acampora D., Pannese M., D'Esposito M., Somma R.,
RA   Gaudino G., Stornaiuolo A., Cafiero M., Faiella A., Simeone A.;
RT   "Organization of human class I homeobox genes.";
RL   Genome 31:745-756(1989).
RN   [5]
RP   NUCLEOTIDE SEQUENCE OF 143-202.
RX   PubMed=2901346; DOI=10.1002/j.1460-2075.1988.tb03052.x;
RA   Kongsuwan K., Webb E., Housiaux P., Adams J.M.;
RT   "Expression of multiple homeobox genes within diverse mammalian
RT   haemopoietic lineages.";
RL   EMBO J. 7:2131-2138(1988).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
RX   PubMed=7876223; DOI=10.1074/jbc.270.9.4544;
RA   Vieille-Grosjean I., Huber P.;
RT   "Transcription factor GATA-1 regulates human HOXB2 gene expression in
RT   erythroid cells.";
RL   J. Biol. Chem. 270:4544-4550(1995).
RN   [7]
RP   FUNCTION, AND GAMMA-GLOBIN PROMOTER AND ENHANCER BINDING FACTOR COMPLEX.
RX   PubMed=10595394; DOI=10.1089/104454999314809;
RA   Case S.S., Huber P., Lloyd J.A.;
RT   "The gammaPE complex contains both SATB1 and HOXB2 and has positive and
RT   negative roles in human gamma-globin gene regulation.";
RL   DNA Cell Biol. 18:805-817(1999).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-274, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
CC   -!- FUNCTION: Sequence-specific transcription factor which is part of a
CC       developmental regulatory system that provides cells with specific
CC       positional identities on the anterior-posterior axis.
CC       {ECO:0000269|PubMed:10595394}.
CC   -!- SUBUNIT: Part of the nuclear protein complex gamma-globin promoter and
CC       enhancer binding factor (gamma-PE) composed at least by SATB1 and
CC       HOXB2. {ECO:0000269|PubMed:10595394}.
CC   -!- INTERACTION:
CC       P14652; Q86UW9: DTX2; NbExp=3; IntAct=EBI-5329558, EBI-740376;
CC       P14652; Q9UJV3-2: MID2; NbExp=3; IntAct=EBI-5329558, EBI-10172526;
CC       P14652; Q6PF18: MORN3; NbExp=3; IntAct=EBI-5329558, EBI-9675802;
CC       P14652; Q9UHB4: NDOR1; NbExp=3; IntAct=EBI-5329558, EBI-10249760;
CC       P14652; Q9BYU1: PBX4; NbExp=3; IntAct=EBI-5329558, EBI-10302990;
CC       P14652; Q06455-2: RUNX1T1; NbExp=3; IntAct=EBI-5329558, EBI-11984663;
CC       P14652; Q9H2K2: TNKS2; NbExp=3; IntAct=EBI-5329558, EBI-4398527;
CC       P14652; Q8TF42: UBASH3B; NbExp=3; IntAct=EBI-5329558, EBI-1380492;
CC       P14652; Q86VK4-3: ZNF410; NbExp=3; IntAct=EBI-5329558, EBI-11741890;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- DEVELOPMENTAL STAGE: Expressed in whole embryos and fetuses at 5-9
CC       weeks from conception.
CC   -!- SIMILARITY: Belongs to the Antp homeobox family. Proboscipedia
CC       subfamily. {ECO:0000305}.
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DR   EMBL; X16665; CAA34655.1; -; mRNA.
DR   EMBL; BC074805; AAH74805.1; -; mRNA.
DR   EMBL; BC074806; AAH74806.1; -; mRNA.
DR   EMBL; X16176; CAA34298.1; -; Genomic_DNA.
DR   EMBL; X14571; CAA32709.1; -; mRNA.
DR   EMBL; X78978; CAA55581.1; -; Genomic_DNA.
DR   CCDS; CCDS11527.1; -.
DR   PIR; S07542; WJHU2H.
DR   RefSeq; NP_002136.1; NM_002145.4.
DR   AlphaFoldDB; P14652; -.
DR   SMR; P14652; -.
DR   BioGRID; 109452; 36.
DR   ELM; P14652; -.
DR   FunCoup; P14652; 1116.
DR   IntAct; P14652; 30.
DR   STRING; 9606.ENSP00000331741; -.
DR   iPTMnet; P14652; -.
DR   PhosphoSitePlus; P14652; -.
DR   BioMuta; HOXB2; -.
DR   DMDM; 123270; -.
DR   MassIVE; P14652; -.
DR   PaxDb; 9606-ENSP00000331741; -.
DR   PeptideAtlas; P14652; -.
DR   Antibodypedia; 30263; 165 antibodies from 30 providers.
DR   DNASU; 3212; -.
DR   Ensembl; ENST00000330070.6; ENSP00000331741.4; ENSG00000173917.11.
DR   GeneID; 3212; -.
DR   KEGG; hsa:3212; -.
DR   MANE-Select; ENST00000330070.6; ENSP00000331741.4; NM_002145.4; NP_002136.1.
DR   UCSC; uc002inm.5; human.
DR   AGR; HGNC:5113; -.
DR   ClinPGx; PA29389; -.
DR   CTD; 3212; -.
DR   DisGeNET; 3212; -.
DR   GeneCards; HOXB2; -.
DR   HGNC; HGNC:5113; HOXB2.
DR   HPA; ENSG00000173917; Low tissue specificity.
DR   MIM; 142967; gene.
DR   OpenTargets; ENSG00000173917; -.
DR   VEuPathDB; HostDB:ENSG00000173917; -.
DR   eggNOG; KOG0489; Eukaryota.
DR   GeneTree; ENSGT00940000155029; -.
DR   HOGENOM; CLU_048378_0_0_1; -.
DR   InParanoid; P14652; -.
DR   OMA; DLPHFNF; -.
DR   OrthoDB; 6159439at2759; -.
DR   PAN-GO; P14652; 4 GO annotations based on evolutionary models.
DR   PhylomeDB; P14652; -.
DR   PathwayCommons; P14652; -.
DR   Reactome; R-HSA-5617472; Activation of anterior HOX genes in hindbrain development during early embryogenesis.
DR   SignaLink; P14652; -.
DR   SIGNOR; P14652; -.
DR   Agora; ENSG00000173917; -.
DR   BioGRID-ORCS; 3212; 8 hits in 1176 CRISPR screens.
DR   ChiTaRS; HOXB2; human.
DR   GeneWiki; HOXB2; -.
DR   GenomeRNAi; 3212; -.
DR   Pharos; P14652; Tbio.
DR   PRO; PR:P14652; -.
DR   Proteomes; UP000005640; Chromosome 17.
DR   RNAct; P14652; protein.
DR   Bgee; ENSG00000173917; Expressed in seminal vesicle and 151 other cell types or tissues.
DR   GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR   GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:NTNU_SB.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR   GO; GO:0007399; P:nervous system development; NAS:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   FunFam; 1.10.10.60:FF:000145; homeobox protein Hox-A2; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR001356; HD.
DR   InterPro; IPR020479; HD_metazoa.
DR   InterPro; IPR001827; Homeobox_Antennapedia_CS.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR009057; Homeodomain-like_sf.
DR   PANTHER; PTHR45664:SF7; HOMEOBOX PROTEIN HOX-B2; 1.
DR   PANTHER; PTHR45664; PROTEIN ZERKNUELLT 1-RELATED; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00024; HOMEOBOX.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00032; ANTENNAPEDIA; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   1: Evidence at protein level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Phosphoprotein;
KW   Proteomics identification; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..356
FT                   /note="Homeobox protein Hox-B2"
FT                   /id="PRO_0000200114"
FT   DNA_BIND        143..202
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          39..142
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          197..240
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           94..99
FT                   /note="Antp-type hexapeptide"
FT   COMPBIAS        52..66
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        77..94
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        106..136
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        227..240
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         274
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   CONFLICT        136..138
FT                   /note="PEA -> RRL (in Ref. 3; CAA34298)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   356 AA;  37914 MW;  D7ACA0E3D5BEBFB9 CRC64;
     MNFEFEREIG FINSQPSLAE CLTSFPAVLE TFQTSSIKES TLIPPPPPFE QTFPSLQPGA
     STLQRPRSQK RAEDGPALPP PPPPPLPAAP PAPEFPWMKE KKSAKKPSQS ATSPSPAASA
     VPASGVGSPA DGLGLPEAGG GGARRLRTAY TNTQLLELEK EFHFNKYLCR PRRVEIAALL
     DLTERQVKVW FQNRRMKHKR QTQHREPPDG EPACPGALED ICDPAEEPAA SPGGPSASRA
     AWEACCHPPE VVPGALSADP RPLAVRLEGA GASSPGCALR GAGGLEPGPL PEDVFSGRQD
     SPFLPDLNFF AADSCLQLSG GLSPSLQGSL DSPVPFSEEE LDFFTSTLCA IDLQFP
//