ID   RR12_CHLRE              Reviewed;         133 AA.
AC   P14149; B7U1K0;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   02-SEP-2026, entry version 145.
DE   RecName: Full=Small ribosomal subunit protein uS12c {ECO:0000255|HAMAP-Rule:MF_00403};
DE   AltName: Full=30S ribosomal protein S12, chloroplastic;
GN   Name=rps12;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], STREPTOMYCIN RESISTANT MUTANTS, AND
RP   MUTAGENESIS OF LYS-14 AND PRO-91.
RX   PubMed=2777778; DOI=10.1016/s0021-9258(18)71592-5;
RA   Liu X.-Q., Gillham N.W., Boynton J.E.;
RT   "Chloroplast ribosomal protein gene rps12 of Chlamydomonas reinhardtii.
RT   Wild-type sequence, mutation to streptomycin resistance and dependence, and
RT   function in Escherichia coli.";
RL   J. Biol. Chem. 264:16100-16108(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Hauser C.R., Boynton J.E., Gillham N.W.;
RL   Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CC-503;
RX   PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA   Smith D.R., Lee R.W.;
RT   "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT   addressing the mutational-hazard hypothesis.";
RL   BMC Evol. Biol. 9:120-120(2009).
RN   [4]
RP   PROTEIN SEQUENCE OF 2-9.
RC   STRAIN=Arg7/cw15;
RX   PubMed=12417713; DOI=10.1105/tpc.004341;
RA   Yamaguchi K., Prieto S., Beligni M.V., Haynes P.A., McDonald W.H.,
RA   Yates J.R. III, Mayfield S.P.;
RT   "Proteomic characterization of the small subunit of Chlamydomonas
RT   reinhardtii chloroplast ribosome: identification of a novel S1 domain-
RT   containing protein and unusually large orthologs of bacterial S2, S3, and
RT   S5.";
RL   Plant Cell 14:2957-2974(2002).
RN   [5]
RP   IDENTIFICATION, AND COMPLETE PLASTID GENOME.
RX   PubMed=12417694; DOI=10.1105/tpc.006155;
RA   Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA   Stern D.B.;
RT   "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT   sea of repeats.";
RL   Plant Cell 14:2659-2679(2002).
CC   -!- FUNCTION: With S4 and S5 plays an important role in translational
CC       accuracy. Located at the interface of the 30S and 50S subunits (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of the 30S ribosomal subunit.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC       {ECO:0000305}.
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DR   EMBL; M29284; AAA84155.1; -; Genomic_DNA.
DR   EMBL; AF061851; AAC16329.1; -; Genomic_DNA.
DR   EMBL; FJ423446; ACJ50147.1; -; Genomic_DNA.
DR   EMBL; BK000554; DAA00960.1; -; Genomic_DNA.
DR   PIR; A34248; A34248.
DR   RefSeq; NP_958416.1; NC_005353.1.
DR   AlphaFoldDB; P14149; -.
DR   EMDB; EMD-56352; -.
DR   EMDB; EMD-56567; -.
DR   EMDB; EMD-56602; -.
DR   SMR; P14149; -.
DR   FunCoup; P14149; 1106.
DR   STRING; 3055.P14149; -.
DR   PaxDb; 3055-P14149; -.
DR   GeneID; 2717036; -.
DR   KEGG; cre:ChreCp060; -.
DR   eggNOG; KOG1750; Eukaryota.
DR   HOGENOM; CLU_104295_1_2_1; -.
DR   InParanoid; P14149; -.
DR   Proteomes; UP000006906; Chloroplast.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005840; C:ribosome; IBA:GO_Central.
DR   GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0006412; P:translation; IBA:GO_Central.
DR   CDD; cd03368; Ribosomal_S12; 1.
DR   FunFam; 2.40.50.140:FF:000001; 30S ribosomal protein S12; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   HAMAP; MF_00403_B; Ribosomal_uS12_B; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR006032; Ribosomal_uS12.
DR   InterPro; IPR005679; Ribosomal_uS12_bact.
DR   NCBIfam; TIGR00981; rpsL_bact; 1.
DR   PANTHER; PTHR11652; 30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER; 1.
DR   Pfam; PF00164; Ribosom_S12_S23; 1.
DR   PIRSF; PIRSF002133; Ribosomal_S12/S23; 1.
DR   PRINTS; PR01034; RIBOSOMALS12.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Chloroplast; Direct protein sequencing; Plastid;
KW   Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW   rRNA-binding.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:12417713"
FT   CHAIN           2..133
FT                   /note="Small ribosomal subunit protein uS12c"
FT                   /id="PRO_0000146396"
FT   MUTAGEN         14
FT                   /note="K->T: Streptomycin resistance."
FT                   /evidence="ECO:0000269|PubMed:2777778"
FT   MUTAGEN         91
FT                   /note="P->L: Streptomycin dependence."
FT                   /evidence="ECO:0000269|PubMed:2777778"
SQ   SEQUENCE   133 AA;  14603 MW;  22A4A5B782EAD62B CRC64;
     MPTIQQLIRS ARKKITKKTK SPALKSCPQR RGICLRVYTV TPKKPNSALR KVARVRLTTG
     FEVTAYIPGV GHNLQEHAVV LVRGGRVKDL PGVRYHIVRG SLDTAGVKNR VQSRSKYGVK
     MGSKTAAKTA GKK
//