ID   RL5_BACSU               Reviewed;         179 AA.
AC   P12877;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   02-SEP-2026, entry version 161.
DE   RecName: Full=Large ribosomal subunit protein uL5 {ECO:0000255|HAMAP-Rule:MF_01333};
DE   AltName: Full=50S ribosomal protein L5 {ECO:0000305};
DE   AltName: Full=BL6;
GN   Name=rplE {ECO:0000255|HAMAP-Rule:MF_01333};
GN   OrderedLocusNames=BSU01280;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Bacillati; Bacillota; Bacilli; Caryophanales; Bacillaceae;
OC   Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=2508062; DOI=10.1093/nar/17.18.7469;
RA   Henkin T.M., Moon S.H., Mattheakis L.C., Nomura M.;
RT   "Cloning and analysis of the spc ribosomal protein operon of Bacillus
RT   subtilis: comparison with the spc operon of Escherichia coli.";
RL   Nucleic Acids Res. 17:7469-7486(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 /
RC   NCIMB 3610 / NRRL NRS-744 / VKM B-501;
RX   PubMed=8635744; DOI=10.1016/0378-1119(95)00757-1;
RA   Suh J.-W., Boylan S.A., Oh S.H., Price C.W.;
RT   "Genetic and transcriptional organization of the Bacillus subtilis spc-
RT   alpha region.";
RL   Gene 169:17-23(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4] {ECO:0007744|PDB:6HA1, ECO:0007744|PDB:6HA8}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.10 ANGSTROMS) OF 1-179 WITH AND WITHOUT
RP   VIRGINIAMYCIN M, INTERACTION WITH VMLR, AND SUBUNIT.
RX   PubMed=30126986; DOI=10.1073/pnas.1808535115;
RA   Crowe-McAuliffe C., Graf M., Huter P., Takada H., Abdelshahid M.,
RA   Novacek J., Murina V., Atkinson G.C., Hauryliuk V., Wilson D.N.;
RT   "Structural basis for antibiotic resistance mediated by the Bacillus
RT   subtilis ABCF ATPase VmlR.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:8978-8983(2018).
RN   [5] {ECO:0007744|PDB:7QV3}
RP   STRUCTURE BY ELECTRON MICROSCOPY (5.14 ANGSTROMS) OF 70S RIBOSOME, AND
RP   INTERACTION WITH MUTS2.
RC   STRAIN=168;
RX   PubMed=35264791; DOI=10.1038/s41586-022-04487-6;
RA   Cerullo F., Filbeck S., Patil P.R., Hung H.C., Xu H., Vornberger J.,
RA   Hofer F.W., Schmitt J., Kramer G., Bukau B., Hofmann K., Pfeffer S.,
RA   Joazeiro C.A.P.;
RT   "Bacterial ribosome collision sensing by a MutS DNA repair ATPase
RT   paralogue.";
RL   Nature 603:509-514(2022).
CC   -!- FUNCTION: This is one of the proteins that bind and probably mediate
CC       the attachment of the 5S RNA into the large ribosomal subunit, where it
CC       forms part of the central protuberance. In the 70S ribosome it contacts
CC       protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits;
CC       this bridge is implicated in subunit movement. Contacts the P site
CC       tRNA; the 5S rRNA and some of its associated proteins might help
CC       stabilize positioning of ribosome-bound tRNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01333}.
CC   -!- SUBUNIT: Part of the 50S ribosomal subunit (PubMed:30126986). Part of
CC       the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site
CC       tRNA. Forms a bridge to the 30S subunit in the 70S ribosome (By
CC       similarity). Interacts with VmlR (PubMed:30126986). In stalled
CC       ribosomes contacts MutS2 (PubMed:35264791). {ECO:0000255|HAMAP-
CC       Rule:MF_01333, ECO:0000269|PubMed:30126986,
CC       ECO:0000269|PubMed:35264791}.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uL5 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01333}.
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DR   EMBL; X15664; CAA33703.1; -; Genomic_DNA.
DR   EMBL; L47971; AAB06811.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11904.1; -; Genomic_DNA.
DR   PIR; S05994; R5BS5.
DR   RefSeq; NP_388009.1; NC_000964.3.
DR   RefSeq; WP_003225809.1; NC_000964.3.
DR   PDB; 3J3V; EM; 13.30 A; F=1-179.
DR   PDB; 3J9W; EM; 3.90 A; BG=1-179.
DR   PDB; 5NJT; EM; 3.80 A; Z=2-179.
DR   PDB; 6HA1; EM; 3.10 A; F=1-179.
DR   PDB; 6HA8; EM; 3.50 A; F=1-179.
DR   PDB; 6HTQ; EM; 4.50 A; F=2-177.
DR   PDB; 6PPK; EM; 4.40 A; F=1-179.
DR   PDB; 6TNN; EM; 3.07 A; Z=1-179.
DR   PDB; 6TPQ; EM; 3.07 A; Z=1-179.
DR   PDB; 7AQC; EM; 2.99 A; F=1-179.
DR   PDB; 7AQD; EM; 3.10 A; F=1-179.
DR   PDB; 7AS8; EM; 2.90 A; H=1-179.
DR   PDB; 7AS9; EM; 3.50 A; H=1-179.
DR   PDB; 7O5B; EM; 3.33 A; c=1-179.
DR   PDB; 7OPE; EM; 3.20 A; H=1-179.
DR   PDB; 7QGU; EM; 4.75 A; F=1-179.
DR   PDB; 7QH4; EM; 5.45 A; F=1-179.
DR   PDB; 7QV1; EM; 3.50 A; F=1-179.
DR   PDB; 7QV2; EM; 3.50 A; F=1-179.
DR   PDB; 7QV3; EM; 5.14 A; F=1-179.
DR   PDB; 8BUU; EM; 2.90 A; F=1-179.
DR   PDB; 8QCQ; EM; 2.30 A; F=1-179.
DR   PDB; 8QPP; EM; 3.40 A; c=2-179.
DR   PDB; 8R55; EM; 3.57 A; c=2-179.
DR   PDB; 8S1P; EM; 1.96 A; F=1-179.
DR   PDB; 8S1U; EM; 3.40 A; F=1-179.
DR   PDB; 9FY1; EM; 2.30 A; F=1-179.
DR   PDB; 9FY2; EM; 2.30 A; F=1-179.
DR   PDB; 9FY3; EM; 2.80 A; F=1-179.
DR   AlphaFoldDB; P12877; -.
DR   EMDB; EMD-0176; -.
DR   EMDB; EMD-0177; -.
DR   EMDB; EMD-0270; -.
DR   EMDB; EMD-10535; -.
DR   EMDB; EMD-10543; -.
DR   EMDB; EMD-11862; -.
DR   EMDB; EMD-11864; -.
DR   EMDB; EMD-11889; -.
DR   EMDB; EMD-11890; -.
DR   EMDB; EMD-12734; -.
DR   EMDB; EMD-13017; -.
DR   EMDB; EMD-14157; -.
DR   EMDB; EMD-14158; -.
DR   EMDB; EMD-14159; -.
DR   EMDB; EMD-16246; -.
DR   EMDB; EMD-18332; -.
DR   EMDB; EMD-19638; -.
DR   EMDB; EMD-19641; -.
DR   EMDB; EMD-3656; -.
DR   EMDB; EMD-50855; -.
DR   EMDB; EMD-50856; -.
DR   EMDB; EMD-50858; -.
DR   SMR; P12877; -.
DR   FunCoup; P12877; 689.
DR   IntAct; P12877; 1.
DR   STRING; 224308.BSU01280; -.
DR   jPOST; P12877; -.
DR   PaxDb; 224308-BSU01280; -.
DR   EnsemblBacteria; CAB11904; CAB11904; BSU_01280.
DR   GeneID; 306025514; -.
DR   GeneID; 86875474; -.
DR   GeneID; 936981; -.
DR   KEGG; bsu:BSU01280; -.
DR   PATRIC; fig|224308.179.peg.131; -.
DR   eggNOG; COG0094; Bacteria.
DR   InParanoid; P12877; -.
DR   OrthoDB; 9806626at2; -.
DR   PhylomeDB; P12877; -.
DR   BioCyc; BSUB:BSU01280-MONOMER; -.
DR   EvolutionaryTrace; P12877; -.
DR   PRO; PR:P12877; -.
DR   Proteomes; UP000001570; Chromosome.
DR   AbasyAtlas; P12877; -.
DR   GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006412; P:translation; IBA:GO_Central.
DR   FunFam; 3.30.1440.10:FF:000001; 50S ribosomal protein L5; 1.
DR   Gene3D; 3.30.1440.10; -; 1.
DR   HAMAP; MF_01333_B; Ribosomal_uL5_B; 1.
DR   InterPro; IPR002132; Ribosomal_uL5.
DR   InterPro; IPR020930; Ribosomal_uL5_bact.
DR   InterPro; IPR031309; Ribosomal_uL5_C.
DR   InterPro; IPR020929; Ribosomal_uL5_CS.
DR   InterPro; IPR022803; Ribosomal_uL5_dom_sf.
DR   InterPro; IPR031310; Ribosomal_uL5_N.
DR   NCBIfam; NF000585; PRK00010.1; 1.
DR   PANTHER; PTHR11994; 60S RIBOSOMAL PROTEIN L11-RELATED; 1.
DR   Pfam; PF00281; Ribosomal_L5; 1.
DR   Pfam; PF00673; Ribosomal_L5_C; 1.
DR   PIRSF; PIRSF002161; Ribosomal_L5; 1.
DR   SUPFAM; SSF55282; RL5-like; 1.
DR   PROSITE; PS00358; RIBOSOMAL_L5; 1.
DR   PDBsum; 3J3V; -.
DR   PDBsum; 3J9W; -.
DR   PDBsum; 5NJT; -.
DR   PDBsum; 6HA1; -.
DR   PDBsum; 6HA8; -.
DR   PDBsum; 6HTQ; -.
DR   PDBsum; 6PPK; -.
DR   PDBsum; 6TNN; -.
DR   PDBsum; 6TPQ; -.
DR   PDBsum; 7AQC; -.
DR   PDBsum; 7AQD; -.
DR   PDBsum; 7AS8; -.
DR   PDBsum; 7AS9; -.
DR   PDBsum; 7O5B; -.
DR   PDBsum; 7OPE; -.
DR   PDBsum; 7QGU; -.
DR   PDBsum; 7QH4; -.
DR   PDBsum; 7QV1; -.
DR   PDBsum; 7QV2; -.
DR   PDBsum; 7QV3; -.
DR   PDBsum; 8BUU; -.
DR   PDBsum; 8QCQ; -.
DR   PDBsum; 8QPP; -.
DR   PDBsum; 8R55; -.
DR   PDBsum; 8S1P; -.
DR   PDBsum; 8S1U; -.
DR   PDBsum; 9FY1; -.
DR   PDBsum; 9FY2; -.
DR   PDBsum; 9FY3; -.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome; Ribonucleoprotein; Ribosomal protein;
KW   RNA-binding; rRNA-binding; tRNA-binding.
FT   CHAIN           1..179
FT                   /note="Large ribosomal subunit protein uL5"
FT                   /id="PRO_0000124892"
FT   HELIX           3..7
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   HELIX           8..12
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   HELIX           13..20
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   HELIX           25..27
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   HELIX           28..30
FT                   /evidence="ECO:0007829|PDB:7AQC"
FT   STRAND          31..39
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   HELIX           41..44
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   HELIX           47..61
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   STRAND          66..69
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   STRAND          70..72
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   HELIX           75..77
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   STRAND          81..83
FT                   /evidence="ECO:0007829|PDB:7AS8"
FT   STRAND          84..91
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   HELIX           93..102
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   HELIX           103..107
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   HELIX           108..110
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   STRAND          111..113
FT                   /evidence="ECO:0007829|PDB:7AQC"
FT   HELIX           116..118
FT                   /evidence="ECO:0007829|PDB:7O5B"
FT   STRAND          124..126
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   STRAND          128..133
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   HELIX           135..137
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   HELIX           139..141
FT                   /evidence="ECO:0007829|PDB:6HA1"
FT   TURN            143..145
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   STRAND          152..159
FT                   /evidence="ECO:0007829|PDB:8S1P"
FT   HELIX           163..173
FT                   /evidence="ECO:0007829|PDB:8S1P"
SQ   SEQUENCE   179 AA;  20148 MW;  57576598B0A7979B CRC64;
     MNRLKEKYNK EIAPALMTKF NYDSVMQVPK IEKIVINMGV GDAVQNAKAI DSAVEELTFI
     AGQKPVVTRA KKSIAGFRLR EGMPIGAKVT LRGERMYDFL DKLISVSLPR VRDFRGVSKK
     SFDGRGNYTL GIKEQLIFPE IDYDKVTKVR GMDIVIVTTA NTDEEARELL TQVGMPFQK
//