ID   ALB3_MAIZE              Reviewed;         303 AA.
AC   P10593;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 2.
DT   02-SEP-2026, entry version 128.
DE   RecName: Full=Albumin b-32;
DE            EC=3.2.2.22;
DE   AltName: Full=Protein opaque-6;
DE   AltName: Full=rRNA N-glycosidase;
GN   Name=O6;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2102870; DOI=10.1007/bf00019399;
RA   Hartings H., Lazzaroni N., Marsan P.A., Aragay A., Thompson R.,
RA   Salamini F., di Fonzo N., Palau J., Motto M.;
RT   "The b-32 protein from maize endosperm: characterization of genomic
RT   sequences encoding two alternative central domains.";
RL   Plant Mol. Biol. 14:1031-1040(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Endosperm;
RX   PubMed=3419419; DOI=10.1007/bf00330853;
RA   di Fonzo N., Hartings H., Brembilla M., Motto M., Soave C., Navarro E.,
RA   Palau J., Rhode W., Salamini F.;
RT   "The b-32 protein from maize endosperm, an albumin regulated by the O2
RT   locus: nucleic acid (cDNA) and amino acid sequences.";
RL   Mol. Gen. Genet. 212:481-487(1988).
CC   -!- FUNCTION: A possible regulatory factor for the synthesis of zeins, the
CC       major group of storage proteins.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- TISSUE SPECIFICITY: Endosperm.
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC       RIP subfamily. {ECO:0000305}.
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DR   EMBL; X54212; CAA38124.1; -; mRNA.
DR   EMBL; X07987; CAA30797.1; -; mRNA.
DR   PIR; S03172; S03172.
DR   AlphaFoldDB; P10593; -.
DR   SMR; P10593; -.
DR   FunCoup; P10593; 12.
DR   STRING; 4577.P10593; -.
DR   PaxDb; 4577-P10593; -.
DR   MaizeGDB; 30000; -.
DR   InParanoid; P10593; -.
DR   Proteomes; UP000007305; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:InterPro.
DR   Gene3D; 3.40.420.10; Ricin (A subunit), domain 1; 1.
DR   Gene3D; 4.10.470.10; Ricin (A Subunit), domain 2; 1.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   InterPro; IPR017989; Ribosome_inactivat_1/2.
DR   InterPro; IPR001574; Ribosome_inactivat_prot.
DR   InterPro; IPR017988; Ribosome_inactivat_prot_CS.
DR   InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR   InterPro; IPR016139; Ribosome_inactivat_prot_sub2.
DR   PANTHER; PTHR33453; -; 1.
DR   PANTHER; PTHR33453:SF9; ALBUMIN B-32; 1.
DR   Pfam; PF00161; RIP; 1.
DR   PRINTS; PR00396; SHIGARICIN.
DR   SUPFAM; SSF56371; Ribosome inactivating proteins (RIP); 1.
DR   PROSITE; PS00275; SHIGA_RICIN; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Hydrolase; Plant defense; Protein synthesis inhibitor;
KW   Reference proteome; Toxin.
FT   CHAIN           1..303
FT                   /note="Albumin b-32"
FT                   /id="PRO_0000221403"
FT   REGION          112..175
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        112..137
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        146..156
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        269
FT                   /note="D -> N (in Ref. 2; CAA30797)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   303 AA;  32428 MW;  24003521CEF91790 CRC64;
     MAETNPELSD LMAQTNKKIV PKFTEIFPVE DVNYPYSAFI ASVRKDVIKH CTDHKGIFQP
     VLPPEKKVPE LWFYTELKTR TSSITLAIRM DNLYLVGFRT PGGVWWELAR PATPTSSATT
     PGGSASAAGT RTSSATRVWR PSPWAARDDQ GRQRPGEEEE DGDTGGGGGA DADADAGGAE
     LAAAAAAADP QADTKSKLVK LVVMVCEGLR FNTLSRTVDA GFNSQHGVTL TVTQGKQVQK
     WDRISKAAFE WADHPTAVIP DMQKLGIKDK NEAARIVALV KNQTTAAAAA ATAASADNDD
     DEA
//