ID   LSRD_ECOLI              Reviewed;         330 AA.
AC   P0AFS1; P76881; P77651;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   02-SEP-2026, entry version 130.
DE   RecName: Full=Autoinducer 2 import system permease protein LsrD;
DE            Short=AI-2 import system permease protein LsrD;
GN   Name=lsrD; Synonyms=ydeZ; OrderedLocusNames=b1515, JW1508;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA   Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA   Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA   Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA   Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA   Wada C., Yamamoto Y., Horiuchi T.;
RT   "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 28.0-40.1 min region on the linkage map.";
RL   DNA Res. 3:363-377(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   FUNCTION IN AI-2 IMPORT, AND INDUCTION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15601708; DOI=10.1128/jb.187.1.238-248.2005;
RA   Xavier K.B., Bassler B.L.;
RT   "Regulation of uptake and processing of the quorum-sensing autoinducer AI-2
RT   in Escherichia coli.";
RL   J. Bacteriol. 187:238-248(2005).
RN   [5]
RP   INDUCTION.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=15743955; DOI=10.1128/jb.187.6.2066-2076.2005;
RA   Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E.;
RT   "Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and
RT   uptake of extracellular autoinducer 2 in Escherichia coli.";
RL   J. Bacteriol. 187:2066-2076(2005).
RN   [6]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: Part of the ABC transporter complex LsrABCD involved in
CC       autoinducer 2 (AI-2) import. Probably responsible for the translocation
CC       of the substrate across the membrane (Probable).
CC       {ECO:0000305|PubMed:15601708}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (LsrA),
CC       two transmembrane proteins (LsrC and LsrD) and a solute-binding protein
CC       (LsrB). {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   -!- INDUCTION: In the absence of AI-2, repressed by LsrR. Induced by AI-2,
CC       via release of the LsrR repressor. In the absence of glucose, induced
CC       by cAMP-CRP by direct binding to the upstream region of the lsr
CC       promoter. {ECO:0000269|PubMed:15601708, ECO:0000269|PubMed:15743955}.
CC   -!- SIMILARITY: Belongs to the binding protein-dependent transport system
CC       permease family. AraH/RbsC subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U00096; AAC74588.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15202.1; -; Genomic_DNA.
DR   PIR; F64905; F64905.
DR   RefSeq; NP_416032.1; NC_000913.3.
DR   RefSeq; WP_001222721.1; NZ_CP009789.1.
DR   AlphaFoldDB; P0AFS1; -.
DR   BioGRID; 4259116; 6.
DR   ComplexPortal; CPX-4315; Autoinducer-2 ABC transporter complex.
DR   FunCoup; P0AFS1; 235.
DR   STRING; 511145.b1515; -.
DR   TCDB; 3.A.1.2.8; the atp-binding cassette (abc) superfamily.
DR   PaxDb; 511145-b1515; -.
DR   EnsemblBacteria; AAC74588; AAC74588; b1515.
DR   GeneID; 75202157; -.
DR   GeneID; 946264; -.
DR   KEGG; ecj:JW1508; -.
DR   KEGG; eco:b1515; -.
DR   KEGG; ecoc:C3026_08760; -.
DR   PATRIC; fig|1411691.4.peg.752; -.
DR   EchoBASE; EB3569; -.
DR   eggNOG; COG1172; Bacteria.
DR   HOGENOM; CLU_028880_0_0_6; -.
DR   InParanoid; P0AFS1; -.
DR   OMA; NTVVFQF; -.
DR   OrthoDB; 7947581at2; -.
DR   PhylomeDB; P0AFS1; -.
DR   BioCyc; EcoCyc:YDEZ-MONOMER; -.
DR   BioCyc; MetaCyc:YDEZ-MONOMER; -.
DR   PHI-base; PHI:9994; -.
DR   PRO; PR:P0AFS1; -.
DR   Proteomes; UP000000625; Chromosome.
DR   AbasyAtlas; P0AFS1; -.
DR   GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; NAS:ComplexPortal.
DR   GO; GO:0016020; C:membrane; NAS:ComplexPortal.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:1905887; P:autoinducer AI-2 transmembrane transport; IDA:EcoCyc.
DR   GO; GO:0009372; P:quorum sensing; NAS:ComplexPortal.
DR   CDD; cd06579; TM_PBP1_transp_AraH_like; 1.
DR   InterPro; IPR001851; ABC_transp_permease.
DR   NCBIfam; NF011612; PRK15038.1; 1.
DR   PANTHER; PTHR32196; ABC TRANSPORTER PERMEASE PROTEIN YPHD-RELATED-RELATED; 1.
DR   PANTHER; PTHR32196:SF71; AUTOINDUCER 2 IMPORT SYSTEM PERMEASE PROTEIN LSRD; 1.
DR   Pfam; PF02653; BPD_transp_2; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..330
FT                   /note="Autoinducer 2 import system permease protein LsrD"
FT                   /id="PRO_0000060248"
FT   TOPO_DOM        1..4
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        26..42
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        64..67
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        110..115
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        137..159
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        160..180
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        181..209
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        210..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        231..237
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        238..258
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        259..279
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        280..285
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        286..306
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        307..330
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   330 AA;  34456 MW;  87CDB0958FB81C1E CRC64;
     MRIRYGWELA LAALLVIEIV AFGAINPRML DLNMLLFSTS DFICIGIVAL PLTMVIVSGG
     IDISFGSTIG LCAIALGVLF QSGVPMPLAI LLTLLLGALC GLINAGLIIY TKVNPLVITL
     GTLYLFAGSA LLLSGMAGAT GYEGIGGFPM AFTDFANLDV LGLPVPLIIF LICLLVFWLW
     LHKTHAGRNV FLIGQSPRVA LYSAIPVNRT LCALYAMTGL ASAVAAVLLV SYFGSARSDL
     GASFLMPAIT AVVLGGANIY GGSGSIIGTA IAVLLVGYLQ QGLQMAGVPN QVSSALSGAL
     LIVVVVGRSV SLHRQQIKEW LARRANNPLP
//