ID   POTB_ECOLI              Reviewed;         275 AA.
AC   P0AFK4; P23860;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   10-JUN-2026, entry version 124.
DE   RecName: Full=Spermidine/putrescine transport system permease protein PotB;
GN   Name=potB; OrderedLocusNames=b1125, JW1111;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=1939142; DOI=10.1016/s0021-9258(18)54799-2;
RA   Furuchi T., Kashiwagi K., Kobayashi H., Igarashi K.;
RT   "Characteristics of the gene for a spermidine and putrescine transport
RT   system that maps at 15 min on the Escherichia coli chromosome.";
RL   J. Biol. Chem. 266:20928-20933(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: Required for the activity of the bacterial periplasmic
CC       transport system of putrescine and spermidine.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the binding protein-dependent transport system
CC       permease family. CysTW subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M64519; AAC37039.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74209.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35945.1; -; Genomic_DNA.
DR   PIR; B40840; B40840.
DR   RefSeq; NP_415643.3; NC_000913.3.
DR   PDB; 8Y5F; EM; 3.13 A; B=1-275.
DR   PDB; 8Y5G; EM; 3.00 A; B=1-269.
DR   PDB; 8Y5H; EM; 3.10 A; B=1-275.
DR   PDB; 8Y5I; EM; 3.00 A; B=1-275.
DR   PDB; 8ZX1; EM; 3.50 A; B=1-275.
DR   AlphaFoldDB; P0AFK4; -.
DR   EMDB; EMD-38933; -.
DR   EMDB; EMD-38936; -.
DR   SMR; P0AFK4; -.
DR   BioGRID; 4261673; 21.
DR   ComplexPortal; CPX-4383; Spermidine ABC transporter complex.
DR   FunCoup; P0AFK4; 292.
DR   STRING; 511145.b1125; -.
DR   TCDB; 3.A.1.11.1; the atp-binding cassette (abc) superfamily.
DR   jPOST; P0AFK4; -.
DR   PaxDb; 511145-b1125; -.
DR   EnsemblBacteria; AAC74209; AAC74209; b1125.
DR   GeneID; 945692; -.
DR   KEGG; ecj:JW1111; -.
DR   KEGG; eco:b1125; -.
DR   PATRIC; fig|511145.12.peg.1171; -.
DR   EchoBASE; EB0743; -.
DR   eggNOG; COG1176; Bacteria.
DR   HOGENOM; CLU_016047_18_3_6; -.
DR   InParanoid; P0AFK4; -.
DR   OMA; VIRTYAW; -.
DR   PhylomeDB; P0AFK4; -.
DR   BioCyc; EcoCyc:POTB-MONOMER; -.
DR   BioCyc; MetaCyc:POTB-MONOMER; -.
DR   PRO; PR:P0AFK4; -.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; TAS:EcoCyc.
DR   GO; GO:0016020; C:membrane; NAS:ComplexPortal.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0015417; F:ABC-type polyamine transporter activity; IDA:EcoCyc.
DR   GO; GO:0015847; P:putrescine transport; IDA:EcoCyc.
DR   GO; GO:1903711; P:spermidine transmembrane transport; IDA:EcoCyc.
DR   CDD; cd06261; TM_PBP2; 1.
DR   FunFam; 1.10.3720.10:FF:000029; Spermidine/putrescine ABC transporter permease PotB; 1.
DR   Gene3D; 1.10.3720.10; MetI-like; 1.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   NCBIfam; NF007044; PRK09497.1; 1.
DR   PANTHER; PTHR42929:SF1; INNER MEMBRANE ABC TRANSPORTER PERMEASE PROTEIN YDCU-RELATED; 1.
DR   PANTHER; PTHR42929; INNER MEMBRANE ABC TRANSPORTER PERMEASE PROTEIN YDCU-RELATED-RELATED; 1.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; MetI-like; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
DR   PDBsum; 8Y5F; -.
DR   PDBsum; 8Y5G; -.
DR   PDBsum; 8Y5H; -.
DR   PDBsum; 8Y5I; -.
DR   PDBsum; 8ZX1; -.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..275
FT                   /note="Spermidine/putrescine transport system permease
FT                   protein PotB"
FT                   /id="PRO_0000060174"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        22..60
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        82..89
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        90..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        111..135
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        136..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        157..187
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        188..208
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        209..241
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        242..262
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        263..275
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          55..261
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   HELIX           1..9
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           10..14
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           15..23
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   STRAND          26..28
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   STRAND          30..36
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           41..44
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           45..47
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           49..80
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   TURN            85..87
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           88..93
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   TURN            94..96
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           97..100
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           103..113
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   STRAND          115..118
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   TURN            119..121
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           122..127
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           133..135
FT                   /evidence="ECO:0007829|PDB:8Y5I"
FT   STRAND          137..139
FT                   /evidence="ECO:0007829|PDB:8Y5H"
FT   HELIX           140..164
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           168..177
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           181..184
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           185..189
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           194..205
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           206..209
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           215..219
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   STRAND          221..223
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           226..235
FT                   /evidence="ECO:0007829|PDB:8Y5G"
FT   HELIX           240..268
FT                   /evidence="ECO:0007829|PDB:8Y5G"
SQ   SEQUENCE   275 AA;  31062 MW;  39D83EF14531D657 CRC64;
     MIVTIVGWLV LFVFLPNLMI IGTSFLTRDD ASFVKMVFTL DNYTRLLDPL YFEVLLHSLN
     MALIATLACL VLGYPFAWFL AKLPHKVRPL LLFLLIVPFW TNSLIRIYGL KIFLSTKGYL
     NEFLLWLGVI DTPIRIMFTP SAVIIGLVYI LLPFMVMPLY SSIEKLDKPL LEAARDLGAS
     KLQTFIRIII PLTMPGIIAG CLLVMLPAMG LFYVSDLMGG AKNLLIGNVI KVQFLNIRDW
     PFGAATSITL TIVMGLMLLV YWRASRLLNK KVELE
//