ID   NUOB_ECOLI              Reviewed;         220 AA.
AC   P0AFC7; P33598; P78090; P78186; P78187;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   02-SEP-2026, entry version 141.
DE   RecName: Full=NADH-quinone oxidoreductase subunit B {ECO:0000255|HAMAP-Rule:MF_01356};
DE            EC=7.1.1.- {ECO:0000255|HAMAP-Rule:MF_01356};
DE   AltName: Full=NADH dehydrogenase I subunit B {ECO:0000255|HAMAP-Rule:MF_01356};
DE   AltName: Full=NDH-1 subunit B {ECO:0000255|HAMAP-Rule:MF_01356};
DE   AltName: Full=NUO2;
GN   Name=nuoB {ECO:0000255|HAMAP-Rule:MF_01356};
GN   OrderedLocusNames=b2287, JW5875;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / AN387;
RX   PubMed=7690854; DOI=10.1006/jmbi.1993.1488;
RA   Weidner U., Geier S., Ptock A., Friedrich T., Leif H., Weiss H.;
RT   "The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase
RT   in Escherichia coli. Organization of the 14 genes and relationship between
RT   the derived proteins and subunits of mitochondrial complex I.";
RL   J. Mol. Biol. 233:109-122(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA   Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA   Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA   Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT   genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT   its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   PROTEIN SEQUENCE OF 1-12.
RC   STRAIN=K12 / EMG2;
RX   PubMed=9298646; DOI=10.1002/elps.1150180807;
RA   Link A.J., Robison K., Church G.M.;
RT   "Comparing the predicted and observed properties of proteins encoded in the
RT   genome of Escherichia coli K-12.";
RL   Electrophoresis 18:1259-1313(1997).
RN   [6]
RP   PROTEIN SEQUENCE OF 1-6.
RX   PubMed=7607227; DOI=10.1111/j.1432-1033.1995.tb20594.x;
RA   Leif H., Sled V.D., Ohnishi T., Weiss H., Friedrich T.;
RT   "Isolation and characterization of the proton-translocating NADH:
RT   ubiquinone oxidoreductase from Escherichia coli.";
RL   Eur. J. Biochem. 230:538-548(1995).
CC   -!- FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur
CC       (Fe-S) centers, to quinones in the respiratory chain. The immediate
CC       electron acceptor for the enzyme in this species is ubiquinone. Couples
CC       the redox reaction to proton translocation (for every two electrons
CC       transferred, four hydrogen ions are translocated across the cytoplasmic
CC       membrane), and thus conserves the redox energy in a proton gradient.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a quinone + NADH + 5 H(+)(in) = a quinol + NAD(+) + 4
CC         H(+)(out); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:132124; Evidence={ECO:0000255|HAMAP-Rule:MF_01356};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01356};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_01356};
CC   -!- SUBUNIT: NDH-1 is composed of 13 different subunits. Subunits NuoB, CD,
CC       E, F, and G constitute the peripheral sector of the complex.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein;
CC       Cytoplasmic side.
CC   -!- SIMILARITY: Belongs to the complex I 20 kDa subunit family.
CC       {ECO:0000255|HAMAP-Rule:MF_01356}.
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DR   EMBL; X68301; CAA48361.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75347.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA16121.2; -; Genomic_DNA.
DR   PIR; E65000; E65000.
DR   RefSeq; NP_416790.1; NC_000913.3.
DR   RefSeq; WP_000386733.1; NZ_CP009789.1.
DR   RefSeq; WP_046608242.1; NZ_LN832404.1.
DR   PDB; 7AWT; EM; 2.73 A; B=1-220.
DR   PDB; 7NYR; EM; 3.30 A; B=1-220.
DR   PDB; 7NYU; EM; 3.80 A; B=1-220.
DR   PDB; 7NYV; EM; 3.70 A; B=1-220.
DR   PDB; 7NZ1; EM; 2.10 A; B=1-220.
DR   PDB; 7P61; EM; 3.20 A; B=1-220.
DR   PDB; 7P62; EM; 3.60 A; B=1-220.
DR   PDB; 7P63; EM; 3.40 A; B=1-220.
DR   PDB; 7P64; EM; 2.50 A; B=1-220.
DR   PDB; 7P69; EM; 3.00 A; B=1-220.
DR   PDB; 7P7C; EM; 2.40 A; B=1-220.
DR   PDB; 7P7E; EM; 2.70 A; B=1-220.
DR   PDB; 7P7J; EM; 2.70 A; B=1-220.
DR   PDB; 7P7K; EM; 3.10 A; B=1-220.
DR   PDB; 7P7L; EM; 3.00 A; B=1-220.
DR   PDB; 7P7M; EM; 3.20 A; B=1-220.
DR   PDB; 7Z7R; EM; 3.36 A; B=1-220.
DR   PDB; 7Z7S; EM; 2.40 A; B=1-220.
DR   PDB; 7Z7T; EM; 3.10 A; B=1-220.
DR   PDB; 7Z7V; EM; 2.29 A; B=1-220.
DR   PDB; 7Z80; EM; 2.93 A; B=1-220.
DR   PDB; 7Z83; EM; 2.88 A; B=1-220.
DR   PDB; 7Z84; EM; 2.87 A; B=1-220.
DR   PDB; 7ZC5; EM; 3.00 A; B=1-220.
DR   PDB; 7ZCI; EM; 2.69 A; B=1-220.
DR   PDB; 9Q8I; EM; 2.35 A; B=1-220.
DR   PDB; 9TAJ; EM; 3.00 A; B=1-220.
DR   PDB; 9TAK; EM; 2.80 A; B=1-220.
DR   PDB; 9TAL; EM; 2.66 A; B=1-220.
DR   PDB; 9TAM; EM; 2.96 A; B=1-220.
DR   PDB; 9TAN; EM; 2.59 A; B=1-220.
DR   AlphaFoldDB; P0AFC7; -.
DR   EMDB; EMD-12653; -.
DR   EMDB; EMD-12654; -.
DR   EMDB; EMD-12655; -.
DR   EMDB; EMD-12661; -.
DR   EMDB; EMD-52895; -.
DR   SMR; P0AFC7; -.
DR   BioGRID; 4262975; 66.
DR   ComplexPortal; CPX-243; Respiratory chain complex I.
DR   FunCoup; P0AFC7; 569.
DR   IntAct; P0AFC7; 1.
DR   STRING; 511145.b2287; -.
DR   TCDB; 3.D.1.1.1; the h+ or na+-translocating nadh dehydrogenase (ndh) family.
DR   jPOST; P0AFC7; -.
DR   PaxDb; 511145-b2287; -.
DR   EnsemblBacteria; AAC75347; AAC75347; b2287.
DR   GeneID; 67176143; -.
DR   GeneID; 93774887; -.
DR   GeneID; 946738; -.
DR   KEGG; ecj:JW5875; -.
DR   KEGG; eco:b2287; -.
DR   KEGG; ecoc:C3026_12760; -.
DR   PATRIC; fig|1411691.4.peg.4449; -.
DR   EchoBASE; EB2008; -.
DR   eggNOG; COG0377; Bacteria.
DR   HOGENOM; CLU_055737_7_3_6; -.
DR   InParanoid; P0AFC7; -.
DR   OMA; CGGPYWE; -.
DR   OrthoDB; 9786737at2; -.
DR   PhylomeDB; P0AFC7; -.
DR   BioCyc; EcoCyc:NUOB-MONOMER; -.
DR   BioCyc; MetaCyc:NUOB-MONOMER; -.
DR   PRO; PR:P0AFC7; -.
DR   Proteomes; UP000000625; Chromosome.
DR   AbasyAtlas; P0AFC7; -.
DR   GO; GO:0016020; C:membrane; IDA:ComplexPortal.
DR   GO; GO:0030964; C:NADH dehydrogenase complex; IDA:EcoliWiki.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoliWiki.
DR   GO; GO:0045271; C:respiratory chain complex I; IDA:EcoCyc.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IMP:EcoCyc.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0050136; F:NADH dehydrogenase (quinone) (non-electrogenic) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR   GO; GO:0048038; F:quinone binding; IEA:InterPro.
DR   GO; GO:0009060; P:aerobic respiration; IMP:EcoCyc.
DR   GO; GO:0015990; P:electron transport coupled proton transport; IMP:EcoCyc.
DR   GO; GO:0022904; P:respiratory electron transport chain; IDA:ComplexPortal.
DR   DisProt; DP03500; -.
DR   FunFam; 3.40.50.12280:FF:000002; NADH-quinone oxidoreductase subunit B; 1.
DR   Gene3D; 3.40.50.12280; -; 1.
DR   HAMAP; MF_01356; NDH1_NuoB; 1.
DR   InterPro; IPR006137; NADH_UbQ_OxRdtase-like_20kDa.
DR   InterPro; IPR006138; NADH_UQ_OxRdtase_20Kd_su.
DR   NCBIfam; TIGR01957; nuoB_fam; 1.
DR   NCBIfam; NF005012; PRK06411.1; 1.
DR   PANTHER; PTHR11995; NADH DEHYDROGENASE; 1.
DR   PANTHER; PTHR11995:SF14; NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL; 1.
DR   Pfam; PF01058; Oxidored_q6; 1.
DR   SUPFAM; SSF56770; HydA/Nqo6-like; 1.
DR   PROSITE; PS01150; COMPLEX1_20K; 1.
DR   PDBsum; 7AWT; -.
DR   PDBsum; 7NYR; -.
DR   PDBsum; 7NYU; -.
DR   PDBsum; 7NYV; -.
DR   PDBsum; 7NZ1; -.
DR   PDBsum; 7P61; -.
DR   PDBsum; 7P62; -.
DR   PDBsum; 7P63; -.
DR   PDBsum; 7P64; -.
DR   PDBsum; 7P69; -.
DR   PDBsum; 7P7C; -.
DR   PDBsum; 7P7E; -.
DR   PDBsum; 7P7J; -.
DR   PDBsum; 7P7K; -.
DR   PDBsum; 7P7L; -.
DR   PDBsum; 7P7M; -.
DR   PDBsum; 7Z7R; -.
DR   PDBsum; 7Z7S; -.
DR   PDBsum; 7Z7T; -.
DR   PDBsum; 7Z7V; -.
DR   PDBsum; 7Z80; -.
DR   PDBsum; 7Z83; -.
DR   PDBsum; 7Z84; -.
DR   PDBsum; 7ZC5; -.
DR   PDBsum; 7ZCI; -.
DR   PDBsum; 9Q8I; -.
DR   PDBsum; 9TAJ; -.
DR   PDBsum; 9TAK; -.
DR   PDBsum; 9TAL; -.
DR   PDBsum; 9TAM; -.
DR   PDBsum; 9TAN; -.
PE   1: Evidence at protein level;
KW   3D-structure; 4Fe-4S; Cell inner membrane; Cell membrane;
KW   Direct protein sequencing; Iron; Iron-sulfur; Membrane; Metal-binding; NAD;
KW   Quinone; Reference proteome; Translocase; Transport; Ubiquinone.
FT   CHAIN           1..220
FT                   /note="NADH-quinone oxidoreductase subunit B"
FT                   /id="PRO_0000118765"
FT   BINDING         63
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01356"
FT   BINDING         64
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01356"
FT   BINDING         129
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01356"
FT   BINDING         158
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01356"
FT   CONFLICT        71
FT                   /note="S -> L (in Ref. 1; CAA48361)"
FT                   /evidence="ECO:0000305"
FT   STRAND          3..6
FT                   /evidence="ECO:0007829|PDB:7Z7V"
FT   STRAND          16..18
FT                   /evidence="ECO:0007829|PDB:7Z7S"
FT   STRAND          20..25
FT                   /evidence="ECO:0007829|PDB:7Z7S"
FT   HELIX           38..51
FT                   /evidence="ECO:0007829|PDB:7Z7V"
FT   STRAND          56..58
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   STRAND          62..64
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   HELIX           65..70
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   HELIX           71..73
FT                   /evidence="ECO:0007829|PDB:7AWT"
FT   TURN            74..76
FT                   /evidence="ECO:0007829|PDB:7Z7V"
FT   HELIX           78..81
FT                   /evidence="ECO:0007829|PDB:7Z7V"
FT   STRAND          87..89
FT                   /evidence="ECO:0007829|PDB:7Z7V"
FT   HELIX           90..92
FT                   /evidence="ECO:0007829|PDB:7P7C"
FT   STRAND          94..98
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   TURN            104..106
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   HELIX           107..116
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   STRAND          122..127
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   HELIX           128..131
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   STRAND          141..143
FT                   /evidence="ECO:0007829|PDB:7P7M"
FT   HELIX           145..147
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   STRAND          152..155
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   STRAND          157..159
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   HELIX           162..176
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   STRAND          178..180
FT                   /evidence="ECO:0007829|PDB:7P61"
FT   STRAND          183..188
FT                   /evidence="ECO:0007829|PDB:7Z7V"
FT   TURN            193..197
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   HELIX           200..203
FT                   /evidence="ECO:0007829|PDB:7NZ1"
FT   HELIX           205..209
FT                   /evidence="ECO:0007829|PDB:7NZ1"
SQ   SEQUENCE   220 AA;  25056 MW;  EBD6268505993880 CRC64;
     MDYTLTRIDP NGENDRYPLQ KQEIVTDPLE QEVNKNVFMG KLNDMVNWGR KNSIWPYNFG
     LSCCYVEMVT SFTAVHDVAR FGAEVLRASP RQADLMVVAG TCFTKMAPVI QRLYDQMLEP
     KWVISMGACA NSGGMYDIYS VVQGVDKFIP VDVYIPGCPP RPEAYMQALM LLQESIGKER
     RPLSWVVGDQ GVYRANMQSE RERKRGERIA VTNLRTPDEI
//