ID   MGPA_ECOLI              Reviewed;         518 AA.
AC   P0AEC0; P76262;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   02-SEP-2026, entry version 130.
DE   RecName: Full=Cation homeostasis protein MgpA {ECO:0000250|UniProtKB:A0A0F6B2D5};
GN   Name=mgpA {ECO:0000250|UniProtKB:A0A0F6B2D5}; Synonyms=yoaE;
GN   OrderedLocusNames=b1816, JW1805;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA   Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   TOPOLOGY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: Involved in cation homeostasis (By similarity). It plays a
CC       role in mitigating excess-cation stress, whether it arises endogenously
CC       or exogenously, most likely by controlling excess Mg(2+) efflux (By
CC       similarity). MgpA may function directly as a cation transporter (By
CC       similarity). {ECO:0000250|UniProtKB:A0A0F6B2D5}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the CorC/HlyC family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U00096; AAC74886.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15623.1; -; Genomic_DNA.
DR   PIR; H64942; H64942.
DR   RefSeq; NP_416330.1; NC_000913.3.
DR   RefSeq; WP_000394983.1; NZ_CP009789.1.
DR   AlphaFoldDB; P0AEC0; -.
DR   SMR; P0AEC0; -.
DR   BioGRID; 4260347; 14.
DR   BioGRID; 850692; 1.
DR   FunCoup; P0AEC0; 1.
DR   IntAct; P0AEC0; 1.
DR   STRING; 511145.b1816; -.
DR   jPOST; P0AEC0; -.
DR   PaxDb; 511145-b1816; -.
DR   EnsemblBacteria; AAC74886; AAC74886; b1816.
DR   GeneID; 93776065; -.
DR   GeneID; 946335; -.
DR   KEGG; ecj:JW1805; -.
DR   KEGG; eco:b1816; -.
DR   KEGG; ecoc:C3026_10340; -.
DR   PATRIC; fig|511145.12.peg.1893; -.
DR   EchoBASE; EB3290; -.
DR   eggNOG; COG1253; Bacteria.
DR   HOGENOM; CLU_015237_0_0_6; -.
DR   InParanoid; P0AEC0; -.
DR   OMA; EFGVIQG; -.
DR   OrthoDB; 9805314at2; -.
DR   PhylomeDB; P0AEC0; -.
DR   BioCyc; EcoCyc:G6997-MONOMER; -.
DR   PRO; PR:P0AEC0; -.
DR   Proteomes; UP000000625; Chromosome.
DR   AbasyAtlas; P0AEC0; -.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   CDD; cd04590; CBS_pair_CorC_HlyC_assoc; 1.
DR   FunFam; 3.10.580.10:FF:000008; Integral membrane protein TerC; 1.
DR   FunFam; 3.30.465.10:FF:000005; Integral membrane protein TerC; 1.
DR   Gene3D; 3.30.465.10; -; 1.
DR   Gene3D; 3.10.580.10; CBS-domain; 1.
DR   InterPro; IPR000644; CBS_dom.
DR   InterPro; IPR046342; CBS_dom_sf.
DR   InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR   InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR   InterPro; IPR005496; Integral_membrane_TerC.
DR   InterPro; IPR044751; Ion_transp-like_CBS.
DR   InterPro; IPR005170; Transptr-assoc_dom.
DR   PANTHER; PTHR22777; HEMOLYSIN-RELATED; 1.
DR   PANTHER; PTHR22777:SF15; UPF0053 INNER MEMBRANE PROTEIN YOAE; 1.
DR   Pfam; PF03471; CorC_HlyC; 1.
DR   Pfam; PF03741; TerC; 1.
DR   SMART; SM01091; CorC_HlyC; 1.
DR   SUPFAM; SSF54631; CBS-domain pair; 1.
DR   SUPFAM; SSF56176; FAD-binding/transporter-associated domain-like; 1.
DR   PROSITE; PS51371; CBS; 2.
PE   1: Evidence at protein level;
KW   CBS domain; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Repeat; Transmembrane; Transmembrane helix.
FT   CHAIN           1..518
FT                   /note="Cation homeostasis protein MgpA"
FT                   /id="PRO_0000088359"
FT   TOPO_DOM        1..12
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        34..48
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        49..69
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        70..78
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        100..124
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        125..145
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        146..149
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        171..184
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        185..205
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        206
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        207..227
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        228..518
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000269|PubMed:15919996"
FT   DOMAIN          304..363
FT                   /note="CBS 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT   DOMAIN          367..427
FT                   /note="CBS 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
SQ   SEQUENCE   518 AA;  56528 MW;  90DF7C9A092F1ECC CRC64;
     MEFLMDPSIW AGLLTLVVLE IVLGIDNLVF IAILADKLPP KQRDKARLLG LSLALIMRLG
     LLSLISWMVT LTKPLFTVMD FSFSGRDLIM LFGGIFLLFK ATTELHERLE NRDHDSGHGK
     GYASFWVVVT QIVILDAVFS LDAVITAVGM VNHLPVMMAA VVIAMAVMLL ASKPLTRFVN
     QHPTVVVLCL SFLLMIGLSL VAEGFGFHIP KGYLYAAIGF SIIIEVFNQI ARRNFIRHQS
     TLPLRARTAD AILRLMGGKR QANVQHDADN PMPMPIPEGA FAEEERYMIN GVLTLASRSL
     RGIMTPRGEI SWVDANLGVD EIREQLLSSP HSLFPVCRGE LDEIIGIVRA KELLVALEEG
     VDVAAIASAS PAIIVPETLD PINLLGVLRR ARGSFVIVTN EFGVVQGLVT PLDVLEAIAG
     EFPDADETPE IITDGDGWLV KGGTDLHALQ QALDVEHLAD DDDIATVAGL VISANGHIPR
     VGDVIDVGPL HITIIEANDY RVDLVRIVKE QPAHDEDE
//