ID   YDJA_ECOLI              Reviewed;         183 AA.
AC   P0ACY1; P24250;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   02-SEP-2026, entry version 127.
DE   RecName: Full=Putative NAD(P)H nitroreductase YdjA;
DE            EC=1.-.-.-;
GN   Name=ydjA; OrderedLocusNames=b1765, JW1754;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1650339; DOI=10.1128/jb.173.16.4983-4993.1991;
RA   Sawers G., Heider J., Zehelein E., Boeck A.;
RT   "Expression and operon structure of the sel genes of Escherichia coli and
RT   identification of a third selenium-containing formate dehydrogenase
RT   isoenzyme.";
RL   J. Bacteriol. 173:4983-4993(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA   Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA   Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA   Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA   Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA   Wada C., Yamamoto Y., Horiuchi T.;
RT   "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 28.0-40.1 min region on the linkage map.";
RL   DNA Res. 3:363-377(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=B / BL21;
RX   PubMed=10493123;
RX   DOI=10.1002/(sici)1522-2683(19990801)20:11<2181::aid-elps2181>3.0.co;2-q;
RA   Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.;
RT   "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite
RT   chromatography.";
RL   Electrophoresis 20:2181-2195(1999).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH FMN, COFACTOR, AND
RP   SUBUNIT.
RX   PubMed=18241886; DOI=10.1016/j.jmb.2008.01.004;
RA   Choi J.-W., Lee J., Nishi K., Kim Y.-S., Jung C.-H., Kim J.-S.;
RT   "Crystal structure of a minimal nitroreductase, ydjA, from Escherichia coli
RT   K12 with and without FMN cofactor.";
RL   J. Mol. Biol. 377:258-267(2008).
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000269|PubMed:18241886};
CC       Note=Binds 1 FMN per subunit. {ECO:0000269|PubMed:18241886};
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:18241886}.
CC   -!- SIMILARITY: Belongs to the nitroreductase family. {ECO:0000305}.
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DR   EMBL; M68961; AAA24245.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74835.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15556.1; -; Genomic_DNA.
DR   PIR; A40360; A40360.
DR   RefSeq; NP_416279.1; NC_000913.3.
DR   RefSeq; WP_000339283.1; NZ_CP009789.1.
DR   PDB; 3BM1; X-ray; 2.00 A; A/B=1-183.
DR   PDB; 3BM2; X-ray; 2.10 A; A/B=1-183.
DR   AlphaFoldDB; P0ACY1; -.
DR   SMR; P0ACY1; -.
DR   BioGRID; 4259139; 171.
DR   DIP; DIP-31841N; -.
DR   FunCoup; P0ACY1; 186.
DR   IntAct; P0ACY1; 11.
DR   STRING; 511145.b1765; -.
DR   jPOST; P0ACY1; -.
DR   PaxDb; 511145-b1765; -.
DR   EnsemblBacteria; AAC74835; AAC74835; b1765.
DR   GeneID; 93775982; -.
DR   GeneID; 945964; -.
DR   KEGG; ecj:JW1754; -.
DR   KEGG; eco:b1765; -.
DR   KEGG; ecoc:C3026_10075; -.
DR   PATRIC; fig|1411691.4.peg.489; -.
DR   EchoBASE; EB1124; -.
DR   eggNOG; COG0778; Bacteria.
DR   HOGENOM; CLU_070764_5_0_6; -.
DR   InParanoid; P0ACY1; -.
DR   OMA; LTEWFAY; -.
DR   OrthoDB; 9804207at2; -.
DR   PhylomeDB; P0ACY1; -.
DR   BioCyc; EcoCyc:EG11134-MONOMER; -.
DR   EvolutionaryTrace; P0ACY1; -.
DR   PRO; PR:P0ACY1; -.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0010181; F:FMN binding; IDA:EcoCyc.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0042803; F:protein homodimerization activity; IDA:EcoCyc.
DR   CDD; cd02135; YdjA-like; 1.
DR   FunFam; 3.40.109.10:FF:000005; NAD(P)H nitroreductase; 1.
DR   Gene3D; 3.40.109.10; NADH Oxidase; 1.
DR   InterPro; IPR029479; Nitroreductase.
DR   InterPro; IPR000415; Nitroreductase-like.
DR   InterPro; IPR026021; YdjA-like.
DR   NCBIfam; NF008088; PRK10828.1; 1.
DR   PANTHER; PTHR43821; NAD(P)H NITROREDUCTASE YDJA-RELATED; 1.
DR   PANTHER; PTHR43821:SF1; NAD(P)H NITROREDUCTASE YDJA-RELATED; 1.
DR   Pfam; PF00881; Nitroreductase; 1.
DR   PIRSF; PIRSF000232; YdjA; 1.
DR   SUPFAM; SSF55469; FMN-dependent nitroreductase-like; 1.
DR   PDBsum; 3BM1; -.
DR   PDBsum; 3BM2; -.
PE   1: Evidence at protein level;
KW   3D-structure; Flavoprotein; FMN; NAD; NADP; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..183
FT                   /note="Putative NAD(P)H nitroreductase YdjA"
FT                   /id="PRO_0000169002"
FT   BINDING         10..12
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /note="in other chain"
FT                   /evidence="ECO:0000269|PubMed:18241886"
FT   BINDING         35
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000269|PubMed:18241886"
FT   BINDING         39
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /evidence="ECO:0000269|PubMed:18241886"
FT   BINDING         121..126
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255"
FT   BINDING         131..133
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /ligand_note="ligand shared between dimeric partners"
FT                   /note="in other chain"
FT                   /evidence="ECO:0000269|PubMed:18241886"
FT   HELIX           3..9
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   STRAND          16..18
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   HELIX           19..21
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   HELIX           23..33
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   STRAND          46..51
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   HELIX           53..69
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   HELIX           73..81
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   HELIX           82..85
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   STRAND          86..94
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   STRAND          100..102
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   HELIX           104..123
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   TURN            124..126
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   STRAND          128..132
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   HELIX           135..138
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   HELIX           140..145
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   STRAND          153..163
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   HELIX           177..179
FT                   /evidence="ECO:0007829|PDB:3BM1"
FT   STRAND          180..182
FT                   /evidence="ECO:0007829|PDB:3BM1"
SQ   SEQUENCE   183 AA;  20059 MW;  A69809EE378847CB CRC64;
     MDALELLINR RSASRLAEPA PTGEQLQNIL RAGMRAPDHK SMQPWHFFVI EGEGRERFSA
     VLEQGAIAAG SDDKAIDKAR NAPFRAPLII TVVAKCEENH KVPRWEQEMS AGCAVMAMQM
     AAVAQGFGGI WRSGALTESP VVREAFGCRE QDKIVGFLYL GTPQLKASTS INVPDPTPFV
     TYF
//