ID   PUNR_ECOLI              Reviewed;         310 AA.
AC   P0ACR2; P37598; P77677;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   02-SEP-2026, entry version 126.
DE   RecName: Full=HTH-type transcriptional regulator PunR {ECO:0000305};
GN   Name=punR {ECO:0000303|PubMed:34413462}; Synonyms=ydhB;
GN   OrderedLocusNames=b1659, JW1651;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / RR28;
RX   PubMed=9022701; DOI=10.1111/j.1432-1033.1996.0712r.x;
RA   Eberhardt S.M.R., Richter G., Gimbel W., Werner T., Bacher A.;
RT   "Cloning, sequencing, mapping and hyperexpression of the ribC gene coding
RT   for riboflavin synthase of Escherichia coli.";
RL   Eur. J. Biochem. 242:712-719(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA   Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA   Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA   Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA   Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA   Wada C., Yamamoto Y., Horiuchi T.;
RT   "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 28.0-40.1 min region on the linkage map.";
RL   DNA Res. 3:363-377(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP   253.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 106-310.
RC   STRAIN=K12;
RX   PubMed=2404765; DOI=10.1111/j.1432-1033.1990.tb15314.x;
RA   Meng L.M., Kilstrup M., Nygaard P.;
RT   "Autoregulation of PurR repressor synthesis and involvement of purR in the
RT   regulation of purB, purC, purL, purMN and guaBA expression in Escherichia
RT   coli.";
RL   Eur. J. Biochem. 187:373-379(1990).
RN   [6]
RP   IDENTIFICATION.
RA   Rudd K.E.;
RL   Unpublished observations (FEB-1994).
RN   [7]
RP   FUNCTION, DNA-BINDING, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12;
RX   PubMed=34413462; DOI=10.1038/s42003-021-02516-0;
RA   Rodionova I.A., Gao Y., Sastry A., Hefner Y., Lim H.G., Rodionov D.A.,
RA   Saier M.H. Jr., Palsson B.O.;
RT   "Identification of a transcription factor, PunR, that regulates the purine
RT   and purine nucleoside transporter punC in E. coli.";
RL   Commun. Biol. 4:991-991(2021).
CC   -!- FUNCTION: Transcriptional regulator that activates the expression of
CC       punC, which encodes a purine nucleoside transporter (PubMed:34413462).
CC       In the presence of adenine, it binds to a conserved 23-bp palindromic
CC       motif in the intergenic region between punR and punC (PubMed:34413462).
CC       {ECO:0000269|PubMed:34413462}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: The deletion of the gene leads to a substantially
CC       decreased growth rate in minimal medium with adenosine, inosine or
CC       guanosine as the nitrogen source. {ECO:0000269|PubMed:34413462}.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; X69109; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; U00096; AAC74731.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15425.2; -; Genomic_DNA.
DR   EMBL; X51368; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; E64923; E64923.
DR   RefSeq; NP_416176.1; NC_000913.3.
DR   RefSeq; WP_000269501.1; NZ_CP009789.1.
DR   AlphaFoldDB; P0ACR2; -.
DR   SMR; P0ACR2; -.
DR   BioGRID; 4262908; 111.
DR   FunCoup; P0ACR2; 36.
DR   IntAct; P0ACR2; 5.
DR   STRING; 511145.b1659; -.
DR   PaxDb; 511145-b1659; -.
DR   EnsemblBacteria; AAC74731; AAC74731; b1659.
DR   GeneID; 93775814; -.
DR   GeneID; 945208; -.
DR   KEGG; ecj:JW1651; -.
DR   KEGG; eco:b1659; -.
DR   KEGG; ecoc:C3026_09520; -.
DR   PATRIC; fig|1411691.4.peg.598; -.
DR   EchoBASE; EB2061; -.
DR   eggNOG; COG0583; Bacteria.
DR   HOGENOM; CLU_039613_35_1_6; -.
DR   InParanoid; P0ACR2; -.
DR   OMA; PKRTTWL; -.
DR   OrthoDB; 5293066at2; -.
DR   PhylomeDB; P0ACR2; -.
DR   BioCyc; EcoCyc:EG12140-MONOMER; -.
DR   PRO; PR:P0ACR2; -.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IBA:GO_Central.
DR   FunFam; 1.10.10.10:FF:000001; LysR family transcriptional regulator; 1.
DR   Gene3D; 3.40.190.10; Periplasmic binding protein-like II; 2.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR000847; LysR_HTH_N.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   NCBIfam; NF008294; PRK11074.1; 1.
DR   PANTHER; PTHR30126; HTH-TYPE TRANSCRIPTIONAL REGULATOR; 1.
DR   PANTHER; PTHR30126:SF18; LYSR FAMILY TRANSCRIPTIONAL REGULATOR; 1.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   SUPFAM; SSF53850; Periplasmic binding protein-like II; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..310
FT                   /note="HTH-type transcriptional regulator PunR"
FT                   /id="PRO_0000105784"
FT   DOMAIN          2..59
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        19..38
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   CONFLICT        253
FT                   /note="K -> E (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   310 AA;  35250 MW;  BBC7A2F2B2AC351A CRC64;
     MWSEYSLEVV DAVARNGSFS AAAQELHRVP SAVSYTVRQL EEWLAVPLFE RRHRDVELTA
     AGAWFLKEGR SVVKKMQITR QQCQQIANGW RGQLAIAVDN IVRPERTRQM IVDFYRHFDD
     VELLVFQEVF NGVWDALSDG RVELAIGATR AIPVGGRYAF RDMGMLSWSC VVASHHPLAL
     MDGPFSDDTL RNWPSLVRED TSRTLPKRIT WLLDNQKRVV VPDWESSATC ISAGLCIGMV
     PTHFAKPWLN EGKWVALELE NPFPDSACCL TWQQNDMSPA LTWLLEYLGD SETLNKEWLR
     EPEETPATGD
//