ID   GLCC_ECOLI              Reviewed;         254 AA.
AC   P0ACL5; P52072; Q2M9L5;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   04-NOV-2026, entry version 131.
DE   RecName: Full=Glc operon transcriptional activator {ECO:0000305|PubMed:8606183};
DE   AltName: Full=Glc regulatory protein {ECO:0000303|PubMed:8606183};
DE   AltName: Full=HTH-type transcriptional regulator GlcC;
GN   Name=glcC {ECO:0000303|PubMed:8606183}; Synonyms=yghN;
GN   OrderedLocusNames=b2980, JW2947;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP   INDUCTION.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8606183; DOI=10.1128/jb.178.7.2051-2059.1996;
RA   Pellicer M.T., Badia J., Aguilar J.T., Baldoma L.;
RT   "glc locus of Escherichia coli: characterization of genes encoding the
RT   subunits of glycolate oxidase and the glc regulator protein.";
RL   J. Bacteriol. 178:2051-2059(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   FUNCTION, AND INDUCTION.
RC   STRAIN=K12 / MC4100;
RX   PubMed=9880556; DOI=10.1074/jbc.274.3.1745;
RA   Pellicer M.T., Fernandez C., Badia J., Aguilar J., Lin E.C., Baldom L.;
RT   "Cross-induction of glc and ace operons of Escherichia coli attributable to
RT   pathway intersection. Characterization of the glc promoter.";
RL   J. Biol. Chem. 274:1745-1752(1999).
CC   -!- FUNCTION: Transcriptional activator of the glcDEFGB operon which is
CC       associated with glycolate utilization, and encodes malate synthase G
CC       and the genes needed for glycolate oxidase activity (PubMed:8606183,
CC       PubMed:9880556). Also negatively regulates the transcription of its own
CC       gene (PubMed:9880556). Glycolate acts as an effector, but GlcC can also
CC       use acetate as an alternative effector (PubMed:9880556).
CC       {ECO:0000269|PubMed:8606183, ECO:0000269|PubMed:9880556}.
CC   -!- INTERACTION:
CC       P0ACL5; P23893: hemL; NbExp=4; IntAct=EBI-1115389, EBI-909193;
CC   -!- INDUCTION: Is constitutively expressed (PubMed:8606183). Is repressed
CC       by the presence of glycolate (PubMed:9880556). GlcC exerts an
CC       autogenous repression on its own gene (PubMed:9880556).
CC       {ECO:0000269|PubMed:8606183, ECO:0000269|PubMed:9880556}.
CC   -!- DISRUPTION PHENOTYPE: Abolishes both glycolate oxidase and malate
CC       synthase G activities. Is unable to grow on glycolate as the sole
CC       source of carbon, in contrast to wild type.
CC       {ECO:0000269|PubMed:8606183}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA69147.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; L43490; AAB02529.1; -; Genomic_DNA.
DR   EMBL; U28377; AAA69147.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00096; AAC76016.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77041.1; -; Genomic_DNA.
DR   PIR; B65084; B65084.
DR   RefSeq; NP_417454.1; NC_000913.3.
DR   RefSeq; WP_001297764.1; NZ_CP009789.1.
DR   AlphaFoldDB; P0ACL5; -.
DR   SMR; P0ACL5; -.
DR   BioGRID; 4262369; 98.
DR   BioGRID; 851784; 56.
DR   FunCoup; P0ACL5; 55.
DR   IntAct; P0ACL5; 65.
DR   STRING; 511145.b2980; -.
DR   PaxDb; 511145-b2980; -.
DR   EnsemblBacteria; AAC76016; AAC76016; b2980.
DR   GeneID; 75205188; -.
DR   GeneID; 947466; -.
DR   KEGG; ecj:JW2947; -.
DR   KEGG; eco:b2980; -.
DR   KEGG; ecoc:C3026_16305; -.
DR   PATRIC; fig|1411691.4.peg.3750; -.
DR   EchoBASE; EB2821; -.
DR   eggNOG; COG2186; Bacteria.
DR   HOGENOM; CLU_017584_9_1_6; -.
DR   InParanoid; P0ACL5; -.
DR   OMA; ACLEHMR; -.
DR   OrthoDB; 5450856at2; -.
DR   PhylomeDB; P0ACL5; -.
DR   BioCyc; EcoCyc:G7546-MONOMER; -.
DR   PRO; PR:P0ACL5; -.
DR   Proteomes; UP000000625; Chromosome.
DR   AbasyAtlas; P0ACL5; -.
DR   GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IDA:EcoCyc.
DR   CDD; cd07377; WHTH_GntR; 1.
DR   FunFam; 1.10.10.10:FF:000338; Glc operon transcriptional activator; 1.
DR   FunFam; 1.20.120.530:FF:000010; Glc operon transcriptional activator; 1.
DR   Gene3D; 1.20.120.530; GntR ligand-binding domain-like; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR011711; GntR_C.
DR   InterPro; IPR008920; TF_FadR/GntR_C.
DR   InterPro; IPR000524; Tscrpt_reg_HTH_GntR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   NCBIfam; NF007442; glcC; 1.
DR   PANTHER; PTHR43537:SF1; GLC OPERON TRANSCRIPTIONAL ACTIVATOR; 1.
DR   PANTHER; PTHR43537; TRANSCRIPTIONAL REGULATOR, GNTR FAMILY; 1.
DR   Pfam; PF07729; FCD; 1.
DR   Pfam; PF00392; GntR; 1.
DR   PRINTS; PR00035; HTHGNTR.
DR   SMART; SM00895; FCD; 1.
DR   SMART; SM00345; HTH_GNTR; 1.
DR   SUPFAM; SSF48008; GntR ligand-binding domain-like; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR   PROSITE; PS50949; HTH_GNTR; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..254
FT                   /note="Glc operon transcriptional activator"
FT                   /id="PRO_0000050645"
FT   DOMAIN          6..74
FT                   /note="HTH gntR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
FT   DNA_BIND        34..53
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
SQ   SEQUENCE   254 AA;  28826 MW;  984C172F18886BF5 CRC64;
     MKDERRPICE VVAESIERLI IDGVLKVGQP LPSERRLCEK LGFSRSALRE GLTVLRGRGI
     IETAQGRDSR VARLNRVQDT SPLIHLFSTQ PRTLYDLLDV RALLEGESAR LAATLGTQAD
     FVVITRCYEK MLAASENNKE ISLIEHAQLD HAFHLAICQA SHNQVLVFTL QSLTDLMFNS
     VFASVNNLYH RPQQKKQIDR QHARIYNAVL QRLPHVAQRA ARDHVRTVKK NLHDIELEGH
     HLIRSAVPLE MNLS
//