ID ARSC_ECOLI Reviewed; 141 AA. AC P0AB96; P37311; Q2M7G5; DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot. DT 08-NOV-2005, sequence version 1. DT 02-SEP-2026, entry version 127. DE RecName: Full=Arsenate reductase; DE EC=1.20.4.1 {ECO:0000250|UniProtKB:P08692}; DE AltName: Full=Arsenical pump modifier; GN Name=arsC {ECO:0000303|PubMed:7721697}; Synonyms=arsG; GN OrderedLocusNames=b3503, JW3470; OS Escherichia coli (strain K12). OC Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; OC Enterobacterales; Enterobacteriaceae; Escherichia. OX NCBI_TaxID=83333; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION. RC STRAIN=K12; RX PubMed=7721697; DOI=10.1128/jb.177.8.2050-2056.1995; RA Diorio C., Cai J., Marmor J., Shinder R., Dubow M.S.; RT "An Escherichia coli chromosomal ars operon homolog is functional in RT arsenic detoxification and is conserved in Gram-negative bacteria."; RL J. Bacteriol. 177:2050-2056(1995). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX PubMed=8041620; DOI=10.1093/nar/22.13.2576; RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.; RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region RT from 76.0 to 81.5 minutes."; RL Nucleic Acids Res. 22:2576-2586(1994). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., RA Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1462(1997). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=16738553; DOI=10.1038/msb4100049; RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 RT and W3110."; RL Mol. Syst. Biol. 2:E1-E5(2006). CC -!- FUNCTION: Involved in resistance to arsenate. Catalyzes the reduction CC of arsenate [As(V)] to arsenite [As(III)]. CC {ECO:0000250|UniProtKB:P08692}. CC -!- CATALYTIC ACTIVITY: CC Reaction=[glutaredoxin]-dithiol + arsenate + glutathione + H(+) = CC glutathionyl-S-S-[glutaredoxin] + arsenite + H2O; CC Xref=Rhea:RHEA:22016, Rhea:RHEA-COMP:10729, Rhea:RHEA-COMP:17668, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29242, CC ChEBI:CHEBI:29950, ChEBI:CHEBI:48597, ChEBI:CHEBI:57925, CC ChEBI:CHEBI:146199; EC=1.20.4.1; CC Evidence={ECO:0000250|UniProtKB:P08692}; CC -!- INDUCTION: Induced by sodium arsenite. {ECO:0000269|PubMed:7721697}. CC -!- SIMILARITY: Belongs to the ArsC family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X80057; CAA56363.1; -; Genomic_DNA. DR EMBL; U00039; AAB18479.1; -; Genomic_DNA. DR EMBL; U00096; AAC76528.1; -; Genomic_DNA. DR EMBL; AP009048; BAE77791.1; -; Genomic_DNA. DR PIR; C56269; C56269. DR RefSeq; NP_417960.1; NC_000913.3. DR RefSeq; WP_000065769.1; NZ_CP009789.1. DR AlphaFoldDB; P0AB96; -. DR SMR; P0AB96; -. DR BioGRID; 4261140; 9. DR FunCoup; P0AB96; 234. DR IntAct; P0AB96; 2. DR STRING; 511145.b3503; -. DR jPOST; P0AB96; -. DR PaxDb; 511145-b3503; -. DR EnsemblBacteria; AAC76528; AAC76528; b3503. DR GeneID; 93778489; -. DR GeneID; 948018; -. DR KEGG; ecj:JW3470; -. DR KEGG; eco:b3503; -. DR KEGG; ecoc:C3026_18975; -. DR PATRIC; fig|1411691.4.peg.3217; -. DR EchoBASE; EB2149; -. DR eggNOG; COG1393; Bacteria. DR HOGENOM; CLU_116644_0_0_6; -. DR InParanoid; P0AB96; -. DR OMA; VCSPKGV; -. DR OrthoDB; 9790554at2; -. DR PhylomeDB; P0AB96; -. DR BioCyc; EcoCyc:EG12237-MONOMER; -. DR PRO; PR:P0AB96; -. DR Proteomes; UP000000625; Chromosome. DR AbasyAtlas; P0AB96; -. DR GO; GO:0008794; F:arsenate reductase (glutaredoxin) activity; ISS:UniProtKB. DR GO; GO:0006974; P:DNA damage response; IEP:EcoliWiki. DR GO; GO:0046685; P:response to arsenic-containing substance; IMP:EcoCyc. DR CDD; cd03034; ArsC_ArsC; 1. DR FunFam; 3.40.30.10:FF:000048; Arsenate reductase; 1. DR Gene3D; 3.40.30.10; Glutaredoxin; 1. DR InterPro; IPR006659; Arsenate_reductase. DR InterPro; IPR006660; Arsenate_reductase-like. DR InterPro; IPR036249; Thioredoxin-like_sf. DR NCBIfam; TIGR00014; arsC; 1. DR NCBIfam; NF007456; PRK10026.1; 1. DR PANTHER; PTHR30041; ARSENATE REDUCTASE; 1. DR PANTHER; PTHR30041:SF5; ARSENATE REDUCTASE-RELATED; 1. DR Pfam; PF03960; ArsC; 1. DR SUPFAM; SSF52833; Thioredoxin-like; 1. DR PROSITE; PS51353; ARSC; 1. PE 2: Evidence at transcript level; KW Arsenical resistance; Oxidoreductase; Reference proteome. FT CHAIN 1..141 FT /note="Arsenate reductase" FT /id="PRO_0000162536" FT ACT_SITE 12 FT /note="Nucleophile; cysteine thioarsenate intermediate" FT /evidence="ECO:0000250|UniProtKB:P08692, FT ECO:0000255|PROSITE-ProRule:PRU01282" FT SITE 8 FT /note="Important for activity" FT /evidence="ECO:0000250|UniProtKB:P08692" FT SITE 60 FT /note="Important for activity" FT /evidence="ECO:0000250|UniProtKB:P08692" FT SITE 94 FT /note="Important for activity" FT /evidence="ECO:0000250|UniProtKB:P08692" FT SITE 107 FT /note="Important for activity" FT /evidence="ECO:0000250|UniProtKB:P08692" SQ SEQUENCE 141 AA; 15853 MW; 5490FBBD14B24D3C CRC64; MSNITIYHNP ACGTSRNTLE MIRNSGTEPT IIHYLETPPT RDELVKLIAD MGISVRALLR KNVEPYEELG LAEDKFTDDR LIDFMLQHPI LINRPIVVTP LGTRLCRPSE VVLEILPDAQ KGAFSKEDGE KVVDEAGKRL K //