ID   ARAG_ECOLI              Reviewed;         504 AA.
AC   P0AAF3; P08531;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   02-SEP-2026, entry version 147.
DE   RecName: Full=Arabinose import ATP-binding protein AraG {ECO:0000255|HAMAP-Rule:MF_01721};
DE            EC=7.5.2.12 {ECO:0000255|HAMAP-Rule:MF_01721};
GN   Name=araG {ECO:0000255|HAMAP-Rule:MF_01721};
GN   OrderedLocusNames=b1900, JW1888;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BEK 180;
RX   PubMed=2445996; DOI=10.1016/0022-2836(87)90607-3;
RA   Scripture J.B., Voelker C., Miller S., O'Donnell R.T., Polgar L., Rade J.,
RA   Horazdovsky B.F., Hogg R.W.;
RT   "High-affinity L-arabinose transport operon. Nucleotide sequence and
RT   analysis of gene products.";
RL   J. Mol. Biol. 197:37-46(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA   Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION IN ARABINOSE TRANSPORT, AND SUBCELLULAR LOCATION.
RC   STRAIN=K12;
RX   PubMed=7028715; DOI=10.1128/jb.148.2.472-479.1981;
RA   Kolodrubetz D., Schleif R.;
RT   "L-arabinose transport systems in Escherichia coli K-12.";
RL   J. Bacteriol. 148:472-479(1981).
RN   [6]
RP   FUNCTION IN ARABINOSE TRANSPORT.
RC   STRAIN=BEK 180;
RX   PubMed=2656640; DOI=10.1128/jb.171.6.3053-3059.1989;
RA   Horazdovsky B.F., Hogg R.W.;
RT   "Genetic reconstitution of the high-affinity L-arabinose transport
RT   system.";
RL   J. Bacteriol. 171:3053-3059(1989).
RN   [7]
RP   INDUCTION.
RX   PubMed=2231717; DOI=10.1016/s0022-2836(05)80163-9;
RA   Hendrickson W., Stoner C., Schleif R.;
RT   "Characterization of the Escherichia coli araFGH and araJ promoters.";
RL   J. Mol. Biol. 215:497-510(1990).
CC   -!- FUNCTION: Part of the ABC transporter complex AraFGH involved in
CC       arabinose import. Responsible for energy coupling to the transport
CC       system (Probable). {ECO:0000305|PubMed:2656640,
CC       ECO:0000305|PubMed:7028715}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-arabinose(out) + ATP + H2O = L-arabinose(in) + ADP +
CC         phosphate + H(+); Xref=Rhea:RHEA:30007, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17535, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.5.2.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01721};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (AraG),
CC       two transmembrane proteins (AraH) and a solute-binding protein (AraF).
CC       {ECO:0000255|HAMAP-Rule:MF_01721}.
CC   -!- INTERACTION:
CC       P0AAF3; P28635: metQ; NbExp=3; IntAct=EBI-559586, EBI-1114713;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01721, ECO:0000269|PubMed:7028715}; Peripheral membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_01721, ECO:0000269|PubMed:7028715}.
CC   -!- INDUCTION: Induced by arabinose. Transcription is dependent on the
CC       transcription factor AraC, the cAMP receptor protein (CRP) and cAMP.
CC       {ECO:0000269|PubMed:2231717}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. Arabinose
CC       importer (TC 3.A.1.2.2) family. {ECO:0000255|HAMAP-Rule:MF_01721}.
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DR   EMBL; X06091; CAA29477.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74970.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15720.1; -; Genomic_DNA.
DR   PIR; S01074; S01074.
DR   RefSeq; NP_416413.1; NC_000913.3.
DR   RefSeq; WP_001187819.1; NZ_CP009789.1.
DR   AlphaFoldDB; P0AAF3; -.
DR   SMR; P0AAF3; -.
DR   BioGRID; 4261851; 6.
DR   BioGRID; 850765; 5.
DR   ComplexPortal; CPX-4314; Arabinose ABC transporter complex.
DR   DIP; DIP-48055N; -.
DR   FunCoup; P0AAF3; 363.
DR   IntAct; P0AAF3; 7.
DR   STRING; 511145.b1900; -.
DR   TCDB; 3.A.1.2.2; the atp-binding cassette (abc) superfamily.
DR   jPOST; P0AAF3; -.
DR   PaxDb; 511145-b1900; -.
DR   EnsemblBacteria; AAC74970; AAC74970; b1900.
DR   GeneID; 75171970; -.
DR   GeneID; 946408; -.
DR   KEGG; ecj:JW1888; -.
DR   KEGG; eco:b1900; -.
DR   KEGG; ecoc:C3026_10785; -.
DR   PATRIC; fig|1411691.4.peg.350; -.
DR   EchoBASE; EB0056; -.
DR   eggNOG; COG1129; Bacteria.
DR   HOGENOM; CLU_000604_92_3_6; -.
DR   InParanoid; P0AAF3; -.
DR   OMA; NVHLGHE; -.
DR   OrthoDB; 9776369at2; -.
DR   PhylomeDB; P0AAF3; -.
DR   BioCyc; EcoCyc:ARAG-MONOMER; -.
DR   BioCyc; MetaCyc:ARAG-MONOMER; -.
DR   PRO; PR:P0AAF3; -.
DR   Proteomes; UP000000625; Chromosome.
DR   AbasyAtlas; P0AAF3; -.
DR   GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; NAS:ComplexPortal.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0016020; C:membrane; NAS:ComplexPortal.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0015612; F:ABC-type L-arabinose transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0016887; F:ATP hydrolysis activity; ISM:EcoCyc.
DR   GO; GO:0015147; F:L-arabinose transmembrane transporter activity; EXP:EcoCyc.
DR   GO; GO:0042882; P:L-arabinose transmembrane transport; NAS:ComplexPortal.
DR   CDD; cd03216; ABC_Carb_Monos_I; 1.
DR   CDD; cd03215; ABC_Carb_Monos_II; 1.
DR   FunFam; 3.40.50.300:FF:000126; Galactose/methyl galactoside import ATP-binding protein MglA; 1.
DR   FunFam; 3.40.50.300:FF:000127; Ribose import ATP-binding protein RbsA; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR050107; ABC_carbohydrate_import_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   NCBIfam; NF008442; PRK11288.1; 1.
DR   PANTHER; PTHR43790:SF6; ARABINOSE IMPORT ATP-BINDING PROTEIN ARAG; 1.
DR   PANTHER; PTHR43790; CARBOHYDRATE TRANSPORT ATP-BINDING PROTEIN MG119-RELATED; 1.
DR   Pfam; PF00005; ABC_tran; 2.
DR   SMART; SM00382; AAA; 2.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
DR   PROSITE; PS51268; ARAG; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW   Nucleotide-binding; Reference proteome; Repeat; Sugar transport;
KW   Translocase; Transport.
FT   CHAIN           1..504
FT                   /note="Arabinose import ATP-binding protein AraG"
FT                   /id="PRO_0000091936"
FT   DOMAIN          8..243
FT                   /note="ABC transporter 1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01721"
FT   DOMAIN          256..499
FT                   /note="ABC transporter 2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01721"
FT   BINDING         40..47
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01721"
SQ   SEQUENCE   504 AA;  55018 MW;  23B401BCF34CFA3A CRC64;
     MQQSTPYLSF RGIGKTFPGV KALTDISFDC YAGQVHALMG ENGAGKSTLL KILSGNYAPT
     TGSVVINGQE MSFSDTTAAL NAGVAIIYQE LHLVPEMTVA ENIYLGQLPH KGGIVNRSLL
     NYEAGLQLKH LGMDIDPDTP LKYLSIGQWQ MVEIAKALAR NAKIIAFDEP TSSLSAREID
     NLFRVIRELR KEGRVILYVS HRMEEIFALS DAITVFKDGR YVKTFTDMQQ VDHDALVQAM
     VGRDIGDIYG WQPRSYGEER LRLDAVKAPG VRTPISLAVR SGEIVGLFGL VGAGRSELMK
     GMFGGTQITA GQVYIDQQPI DIRKPSHAIA AGMMLCPEDR KAEGIIPVHS VRDNINISAR
     RKHVLGGCVI NNGWEENNAD HHIRSLNIKT PGAEQLIMNL SGGNQQKAIL GRWLSEEMKV
     ILLDEPTRGI DVGAKHEIYN VIYALAAQGV AVLFASSDLP EVLGVADRIV VMREGEIAGE
     LLHEQADERQ ALSLAMPKVS QAVA
//