ID   MCAPS_TYYVF             Reviewed;         670 AA.
AC   P09514;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   04-NOV-2026, entry version 100.
DE   RecName: Full=Readthrough protein P3-RTD;
DE   AltName: Full=P3-P5 readthrough protein {ECO:0000250|UniProtKB:P17525};
DE   AltName: Full=P74;
DE   AltName: Full=Readthrough protein;
DE            Short=RT protein;
DE   Contains:
DE     RecName: Full=Minor capsid readthrough protein {ECO:0000303|PubMed:20416918};
DE              Short=Minor capsid RT protein {ECO:0000303|PubMed:20416918};
DE     AltName: Full=54 kDa protein;
DE   Contains:
DE     RecName: Full=Cleaved product {ECO:0000305};
GN   ORFNames=ORF3/ORF5;
OS   Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Sobelivirales; Solemoviridae; Polerovirus; Polerovirus BWYV;
OC   Beet western yellows virus.
OX   NCBI_TaxID=12043;
OH   NCBI_TaxID=161934; Beta vulgaris (Sugar beet).
OH   NCBI_TaxID=3709; Brassica napus subsp. rapifera (rutabaga).
OH   NCBI_TaxID=138011; Brassica napus var. napus (canola).
OH   NCBI_TaxID=3710; Brassica nigra (Black mustard) (Sinapis nigra).
OH   NCBI_TaxID=3715; Brassica oleracea var. botrytis (Cauliflower).
OH   NCBI_TaxID=3716; Brassica oleracea var. capitata (Cabbage).
OH   NCBI_TaxID=51350; Brassica rapa subsp. rapa (Turnip).
OH   NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH   NCBI_TaxID=3827; Cicer arietinum (Chickpea) (Garbanzo).
OH   NCBI_TaxID=3654; Citrullus lanatus (Watermelon) (Citrullus vulgaris).
OH   NCBI_TaxID=3721; Crambe hispanica subsp. abyssinica (Abyssinian kale) (Crambe abyssinica).
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH   NCBI_TaxID=3663; Cucurbita pepo (Vegetable marrow) (Summer squash).
OH   NCBI_TaxID=3847; Glycine max (Soybean) (Glycine hispida).
OH   NCBI_TaxID=4232; Helianthus annuus (Common sunflower).
OH   NCBI_TaxID=4236; Lactuca sativa (Garden lettuce).
OH   NCBI_TaxID=103529; Phlox drummondii (Annual phlox).
OH   NCBI_TaxID=3888; Pisum sativum (Garden pea) (Lathyrus oleraceus).
OH   NCBI_TaxID=3726; Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH   NCBI_TaxID=3562; Spinacia oleracea (Spinach).
OH   NCBI_TaxID=3900; Trifolium subterraneum (Subterranean clover).
OH   NCBI_TaxID=3906; Vicia faba (Broad bean) (Faba vulgaris).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3194229; DOI=10.1093/nar/16.21.9917;
RA   Veidt I., Lot H., Leiser M., Scheidecker D., Guilley H., Richards K.E.,
RA   Jonard G.;
RT   "Nucleotide sequence of beet western yellows virus RNA.";
RL   Nucleic Acids Res. 16:9917-9932(1988).
RN   [2]
RP   FUNCTION (MINOR CAPSID READTHROUGH PROTEIN).
RX   PubMed=7882968; DOI=10.1002/j.1460-2075.1995.tb07043.x;
RA   Brault V., van den Heuvel J.F., Verbeek M., Ziegler-Graff V.,
RA   Reutenauer A., Herrbach E., Garaud J.-C., Guilley H., Richards K.E.,
RA   Jonard G.;
RT   "Aphid transmission of beet western yellows luteovirus requires the minor
RT   capsid read-through protein P74.";
RL   EMBO J. 14:650-659(1995).
RN   [3]
RP   DOMAIN (READTHROUGH PROTEIN P3-RTD).
RX   PubMed=9143288; DOI=10.1006/viro.1997.8476;
RA   Bruyere A., Brault V., Ziegler-Graff V., Simonis M.T., Van den Heuvel J.F.,
RA   Richards K., Guilley H., Jonard G., Herrbach E.;
RT   "Effects of mutations in the beet western yellows virus readthrough protein
RT   on its expression and packaging and on virus accumulation, symptoms, and
RT   aphid transmission.";
RL   Virology 230:323-334(1997).
RN   [4]
RP   MUTAGENESIS OF ARG-227; 262-GLU-ASP-263; 316-LYS-ASP-317; 403-LYS-TYR-404
RP   AND 428-ASP-GLU-429, AND FUNCTION (MINOR CAPSID READTHROUGH PROTEIN).
RX   PubMed=10627524; DOI=10.1128/jvi.74.3.1140-1148.2000;
RA   Brault V., Mutterer J., Scheidecker D., Simonis M.T., Herrbach E.,
RA   Richards K.E., Ziegler-Graff V.;
RT   "Effects of point mutations in the readthrough domain of the beet western
RT   yellows virus minor capsid protein on virus accumulation in planta and on
RT   transmission by aphids.";
RL   J. Virol. 74:1140-1148(2000).
RN   [5]
RP   ABSENCE OF GLYCOSYLATION (READTHROUGH PROTEIN P3-RTD), PROTEOLYTIC CLEAVAGE
RP   (READTHROUGH PROTEIN P3-RTD), AND FUNCTION (MINOR CAPSID READTHROUGH
RP   PROTEIN).
RX   PubMed=20416918; DOI=10.1016/j.virol.2010.03.037;
RA   Revollon S., Strub J.M., Fitchette A.C., Wiss L., Gomord V.,
RA   Van Dorsselaer A., Brault V.;
RT   "A reinvestigation provides no evidence for sugar residues on structural
RT   proteins of poleroviruses and argues against a role for glycosylation of
RT   virus structural proteins in aphid transmission.";
RL   Virology 402:303-314(2010).
RN   [6]
RP   FUNCTION (MINOR CAPSID READTHROUGH PROTEIN).
RX   PubMed=26402374; DOI=10.1016/j.virol.2015.08.031;
RA   Rodriguez-Medina C., Boissinot S., Chapuis S., Gereige D., Rastegar M.,
RA   Erdinger M., Revers F., Ziegler-Graff V., Brault V.;
RT   "A protein kinase binds the C-terminal domain of the readthrough protein of
RT   Turnip yellows virus and regulates virus accumulation.";
RL   Virology 486:44-53(2015).
CC   -!- FUNCTION: [Minor capsid readthrough protein]: Minor component of the
CC       viral capsid involved in aphid transmission and virus accumulation in
CC       the host (PubMed:10627524, PubMed:20416918, PubMed:26402374,
CC       PubMed:7882968). Required for the virus to move through the aphid (By
CC       similarity). The RTD domain of the protein is exposed on the surface of
CC       the particle and determines the vector specificity and intestinal
CC       tropism in the aphid (PubMed:7882968). {ECO:0000250|UniProtKB:P09516,
CC       ECO:0000269|PubMed:10627524, ECO:0000269|PubMed:20416918,
CC       ECO:0000269|PubMed:26402374, ECO:0000269|PubMed:7882968}.
CC   -!- SUBCELLULAR LOCATION: [Minor capsid readthrough protein]: Virion
CC       {ECO:0000250|UniProtKB:P17525}. Host cell junction, host plasmodesma
CC       {ECO:0000250|UniProtKB:P17525}. Host periplasm
CC       {ECO:0000250|UniProtKB:P17525}.
CC   -!- SUBCELLULAR LOCATION: [Cleaved product]: Virion
CC       {ECO:0000250|UniProtKB:P17525}.
CC   -!- DOMAIN: [Readthrough protein P3-RTD]: The N-terminus is highly basic
CC       like those of many plant virus capsid proteins (Probable). These
CC       regions may be involved in protein-RNA interaction (Probable). The RTD
CC       N-terminus is responsible for aphid transmission and aphid endosymbiont
CC       interaction (PubMed:9143288). The readthrough domain is required for
CC       transport of virus through membranes of the aphid salivary glands (By
CC       similarity). {ECO:0000250|UniProtKB:P09516, ECO:0000269|PubMed:9143288,
CC       ECO:0000305}.
CC   -!- PTM: [Readthrough protein P3-RTD]: In virus particles, more than 200
CC       amino acids are proteolytically cleaved releasing the C-terminus part
CC       of the RTD domain (PubMed:20416918). The cleaved product remains
CC       attached to the virus particle (By similarity).
CC       {ECO:0000250|UniProtKB:P17525, ECO:0000269|PubMed:20416918}.
CC   -!- MISCELLANEOUS: [Readthrough protein P3-RTD]: This protein is translated
CC       as a fusion protein by episodic readthrough of the major coat protein
CC       termination codon. It is composed of the major capsid protein fused to
CC       a long C-terminal extension called the readthrough domain (RTD).
CC       Readthrough of the terminator codon TAG occurs between the codons for
CC       202-Lys and 204-Val. {ECO:0000250|UniProtKB:P17525}.
CC   -!- SIMILARITY: Belongs to the luteoviruses readthrough protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA31467.2; Type=Miscellaneous discrepancy; Note=Readthrough of an in-frame TAG stop codon in position 203 translated as Tyr.; Evidence={ECO:0000305};
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DR   EMBL; X13063; CAA31467.2; ALT_SEQ; Genomic_RNA.
DR   PIR; S01943; S01943.
DR   RefSeq; NP_620487.1; NC_003743.1.
DR   PDB; 7ULN; X-ray; 1.53 A; A/B=224-461.
DR   PDB; 9FHP; EM; 4.08 A; A/B/C=1-202.
DR   PDB; 9Q8J; EM; 3.47 A; A/B/C=1-202.
DR   SMR; P09514; -.
DR   GeneID; 940480; -.
DR   KEGG; vg:940480; -.
DR   InterPro; IPR001517; Luteo_coat.
DR   InterPro; IPR002929; PLrV_ORF5.
DR   Pfam; PF00894; Luteo_coat; 1.
DR   Pfam; PF01690; PLRV_ORF5; 1.
DR   PRINTS; PR00910; LVIRUSORF6.
DR   GO; GO:0044229; C:host cell periplasmic space; ISS:UniProtKB.
DR   GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   Proteomes; UP000007545; Segment.
DR   EMDB; EMD-10003; -.
PE   1: Evidence at protein level;
KW   3D-structure; Capsid protein; Host cell junction; Host periplasm;
KW   Reference proteome; RNA suppression of termination; Virion.
FT   CHAIN           1..670
FT                   /note="Readthrough protein P3-RTD"
FT                   /id="PRO_0000222426"
FT   CHAIN           1..437
FT                   /note="Minor capsid readthrough protein"
FT                   /id="PRO_0000455349"
FT   CHAIN           438..670
FT                   /note="Cleaved product"
FT                   /id="PRO_0000455350"
FT   REGION          1..64
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          203..223
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          204..670
FT                   /note="Readthrough domain (RTD)"
FT   REGION          431..553
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          609..629
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          641..670
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        10..24
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        25..36
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        38..49
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        50..60
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        209..221
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        466..485
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        536..545
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        609..624
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            437..438
FT                   /note="Cleavage"
FT                   /evidence="ECO:0000269|PubMed:20416918"
FT   MUTAGEN         227
FT                   /note="R->A: Complete loss of aphid transmission."
FT                   /evidence="ECO:0000269|PubMed:10627524"
FT   MUTAGEN         262..263
FT                   /note="ED->A: Complete loss of aphid transmission."
FT                   /evidence="ECO:0000269|PubMed:10627524"
FT   MUTAGEN         316..317
FT                   /note="KD->A: No effect on aphid transmission."
FT                   /evidence="ECO:0000269|PubMed:10627524"
FT   MUTAGEN         403..404
FT                   /note="KY->A: Complete loss of aphid transmission."
FT                   /evidence="ECO:0000269|PubMed:10627524"
FT   MUTAGEN         428..429
FT                   /note="DE->A: Almost complete loss of aphid transmission."
FT                   /evidence="ECO:0000269|PubMed:10627524"
FT   STRAND          66..75
FT                   /evidence="ECO:0007829|PDB:9Q8J"
FT   STRAND          79..85
FT                   /evidence="ECO:0007829|PDB:9Q8J"
FT   HELIX           94..98
FT                   /evidence="ECO:0007829|PDB:9Q8J"
FT   HELIX           99..101
FT                   /evidence="ECO:0007829|PDB:9Q8J"
FT   STRAND          102..115
FT                   /evidence="ECO:0007829|PDB:9Q8J"
FT   STRAND          125..131
FT                   /evidence="ECO:0007829|PDB:9Q8J"
FT   STRAND          143..147
FT                   /evidence="ECO:0007829|PDB:9Q8J"
FT   STRAND          152..156
FT                   /evidence="ECO:0007829|PDB:9Q8J"
FT   HELIX           159..161
FT                   /evidence="ECO:0007829|PDB:9Q8J"
FT   STRAND          174..180
FT                   /evidence="ECO:0007829|PDB:9Q8J"
FT   STRAND          184..201
FT                   /evidence="ECO:0007829|PDB:9Q8J"
FT   STRAND          228..233
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          236..241
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   HELIX           251..255
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          256..262
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          265..271
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          273..278
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          280..290
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          293..305
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          307..309
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          319..323
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          331..355
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          371..384
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          386..388
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          390..394
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   HELIX           402..404
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          414..419
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          421..429
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   HELIX           446..449
FT                   /evidence="ECO:0007829|PDB:7ULN"
FT   STRAND          450..452
FT                   /evidence="ECO:0007829|PDB:7ULN"
SQ   SEQUENCE   670 AA;  74016 MW;  4301B21EE7B4BE9F CRC64;
     MNTVVGRRII NGRRRPRRQT RRAQRPQPVV VVQTSRATQR RPRRRRRGNN RTGRTVPTRG
     AGSSETFVFS KDNLAGSSSG AITFGPSLSD CPAFSNGMLK AYHEYKISMV ILEFVSEASS
     QNSGSIAYEL DPHCKLNSLS STINKFGITK PGKRAFTASY INGTEWHDVA EDQFRILYKG
     NGSSSIAGSF RITIKCQFHN PKYVDEEPGP SPGPSPSPQP TPQKKYRFIV YTGVPVTRIM
     AQSTDDAISL YDMPSQRFRY IEDENMNWTN LDSRWYSQNS LKAIPMIIVP VPQGEWTVEI
     SMEGYQPTSS TTDPNKDKQD GLIAYNDDLS EGWNVGIYNN VEITNNKADN TLKYGHPDME
     LNGCHFNQGQ CLERDGDLTC HIKTTGDNAS FFVVGPAVQK QSKYNYAVSY GAWTDRMMEI
     GMIAIALDEQ GSSGSVKTER PKRVGHSMAV STWETIKLPE KGNSEGYETS QRQDSKTPPT
     ASGGSDTLDV EEGGLPLPVE EEIPDFVGDN PWSDLSTKNS QEEEAMSSES GLRPQLKPPG
     LPKPQPIRTI RNFDPTPDLV EAWRPDVNPG YSKADVAAAT IIAGGSIKDG RSMIDKRNKA
     VLDGRKSWGS SLASSLTGGT LKASAKSEKL AKLTTSERAR YERIKRQQGS TRASEFLESL
     LAGEDPDSRF
//